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Nerve tissue engineering electric conduction nano-fiber tubular stent and preparation method thereof

A nerve tissue and fiber tube technology is applied in the fields of conductive nanofiber tubular scaffolds, nerve conduits for nerve repair and their preparation, new tissue engineering filling materials and preparation fields, and achieves the promotion of proliferation and differentiation, excellent conductivity, and high packing density. Effect

Inactive Publication Date: 2017-09-12
BEIJING UNIV OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no catheter scaffold material that can provide a multifunctional biomimetic microenvironment for neural tissue engineering

Method used

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  • Nerve tissue engineering electric conduction nano-fiber tubular stent and preparation method thereof
  • Nerve tissue engineering electric conduction nano-fiber tubular stent and preparation method thereof
  • Nerve tissue engineering electric conduction nano-fiber tubular stent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Dissolve 4 g of PY and 10 g of DBSA in 100 ml of deionized water, place in an ice bath, and add 10 ml of pre-cooled APS aqueous solution [20% (w / v)] dropwise under mechanical stirring , Initiate PY polymerization in PY / DBSA solution under mechanical stirring, control the reaction temperature at 0°C, add excess methanol after 8h to terminate the reaction, collect the black precipitate by centrifugation, wash with deionized water, methanol, acetone, respectively, until liquid Become colorless and transparent. The black solid product was dissolved in chloroform, filtered to remove the insoluble matter, and the solvent was removed by rotary evaporation to prepare soluble PPY.

[0038] (2) Dissolve 2 g PLGA7525 (lactide content 75 mol%, molecular weight 100,000) in 5.5 ml chloroform, dissolve 0.2 g PPY prepared in step (1) in 5.5 ml DMF, and mix the two in equal proportions to obtain the mass A mixed solution with a concentration of 20% by volume, with a metal drum rota...

Embodiment 2

[0046] Adopt the same method as Example 1 to prepare the conductive fiber tubular support filled with conductive hydrogel, except the outer biodegradable polyester non-woven fiber membrane of the tubular support, adopt poly-L-lactic acid (PLLA, molecular weight) whose degradation rate is slower than PLGA7525 100,000), thereby obtaining a NGF-loaded conductive fiber tubular scaffold for nerve tissue engineering with a certain degradation gradient from the inside to the outside of the fiber tube wall.

[0047] Step (4) is operated as follows, and other operating steps are the same as in Example 1:

[0048] Dissolve 1 g of PLLA in 10 ml of trifluoroethanol to obtain a viscous solution with a mass volume percentage concentration of 10%. The aluminum foil fixed with parallel fiber membrane A is used as the receiving device, and the fibers are directly piled up on it by electrospinning and arranged randomly The double-layer spun fiber membrane C was obtained from the PLLA fiber memb...

Embodiment 3

[0050] The conductive fiber tubular scaffold filled with conductive hydrogel was prepared by the same method as in Example 1, except that the growth factor aqueous solution configured in step (7) was a solution containing both NGF and bFGF, thus obtaining a Conductive fiber tubular scaffolds for neural tissue engineering with growth factors that promote nerve regeneration.

[0051] Step (7) is operated as follows, and other operating steps are the same as in Example 1:

[0052] The conductive PPY nanotubes prepared in step (6) are treated by the method of modifying the dopamine aqueous solution in step (3), fully ground after drying and sterilized by ultraviolet light. Dissolve 0.01 mg NGF and 0.01 mg bFGF in 10 ml deionized water at the same time, filter and sterilize, disperse and impregnate 0.1 g PPY nanotubes in the NGF aqueous solution after sterilizing, and load the two via the adsorption of nanotubes. a growth factor.

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Abstract

The present invention relates to a nerve tissue engineering electric conduction nano-fiber tubular stent and a preparation method thereof. The nerve tissue engineering electric conduction nano-fiber tubular stent comprises an electric conduction nano-fiber tube and an electric conduction hydrogel filling material, wherein the electric conduction nano-fiber tube is formed by compounding an inner layer and an outer layer, the inner layer is an electric conduction nano-fiber film arranged in a parallel manner and oriented along the axial direction of the catheter, the outer layer is a non-electric conduction non-woven arrangement nano-fiber film, and the filling material is formed by compounding a natural macromolecular hydrogel and growth factor-loading electric conduction nano-tubes. According to the present invention, the catheter stent material can provide the multifunctional bionic microenvironment for nerve tissue engineering, has good biocompatibility, and can provide nutrients for nerve repair, wherein the two-dimensionally oriented fiber morphology can promote the differentiation and growth of nerve cells, and with the adding of the electric conduction material, the differentiation degree of nerve cells can be further improved, the cell proliferation can be promoted, and the conditions required by nerve regeneration can be well met.

Description

technical field [0001] The invention relates to the field of biomaterials, and relates to a nerve conduit for nerve repair and a preparation method thereof, in particular to a conductive nanofiber tubular support and a new tissue engineering filling material used in the tube to promote nerve regeneration and a preparation method thereof. Background technique [0002] Peripheral nerve tissue damage due to severe trauma, tumor resection, etc. is very common in clinical practice. Once the nerve is damaged, the patient will not be able to live and work normally, but the repair and functional reconstruction of large nerve defects are still clinical problems. Nerve transplantation is clinically recognized as an effective method for treating large-scale nerve defects, but the source of autologous nerves is limited, and it is difficult to avoid immune rejection of allogeneic nerves, and the repair effect is limited when skeletal muscle or veins are used instead of nerves for transpl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/18A61L27/20A61L27/38A61L27/54A61L27/50
Inventor 兰金叻景伟蔡晴杨小平陈国强王林
Owner BEIJING UNIV OF CHEM TECH
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