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Preparation method of degradable piezoelectric fiber stent and product thereof

A technology of piezoelectric fibers and fiber membranes, which is applied in the field of preparation of degradable piezoelectric fiber scaffolds, can solve the problems of conductive hydrogel biocompatibility and poor material degradability, and achieve suitable cell attachment, simple method, and cell The effect of low toxicity

Active Publication Date: 2021-09-21
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current problems of poor biocompatibility and material degradability of conductive hydrogels, the present invention provides a degradable piezoelectric fiber scaffold, which can stimulate neural stem cells under the electrical stimulation generated under the action of ultrasound and the three-dimensional structure of the scaffold itself. Differentiation promotes

Method used

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  • Preparation method of degradable piezoelectric fiber stent and product thereof
  • Preparation method of degradable piezoelectric fiber stent and product thereof
  • Preparation method of degradable piezoelectric fiber stent and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of degradable piezoelectric fiber scaffold

[0031] (1) Dissolve gelatin powder in a mixed solution of N,N-dimethylformamide: formic acid = 1:9 (v / v) to prepare a gelatin solution with a mass fraction of 20%; use a syringe to draw about 5mL of gelatin solution, insert a needle with a specification of 21G, and place it in a spinning machine; set the spinning parameters: distance 12cm, voltage 18kV. After spinning, the fiber membrane was removed and dried in an oven at 40° C. for 30 minutes. The SEM image of the fiber membrane is shown in figure 1 As shown, it can be seen from the SEM image that the surface of the gelatin fiber is smooth and uniform in thickness, and the diameter of the fiber filament is about 150 nm.

[0032] (2) Place the gelatin fiber membrane in saturated glutaraldehyde steam, cross-link for 24 hours at 25°C, take it out after cross-linking, place it in a fume hood for 30 minutes to volatilize the glutaraldehyde and dry it to o...

Embodiment 2

[0033] Example 2 Preparation of degradable piezoelectric fiber scaffold

[0034] (1) Dissolve gelatin powder in a mixed solution of N,N-dimethylformamide:formic acid=4:6 (v / v), and prepare a gelatin solution with a mass fraction of 30%; use a syringe to draw about 5mL of gelatin solution, insert a needle with a specification of 21G, and place it in a spinning machine; set the spinning parameters: distance 12cm, voltage 18kV. After spinning, the fiber membrane was removed and dried in an oven at 40° C. for 30 minutes. The SEM image of the fiber membrane is shown in figure 1 As shown, it can be seen from the SEM image that the surface of the gelatin fiber is smooth and uniform in thickness, and the diameter of the fiber filament is between 200nm and 500nm.

[0035] (2) Place the gelatin fiber membrane in saturated glutaraldehyde steam, cross-link for 24 hours at 25°C, take it out after cross-linking and place it in a fume hood for 30 minutes to volatilize the glutaraldehyde an...

Embodiment 3

[0036] Example 3 Preparation of degradable piezoelectric fiber scaffold

[0037] (1) Dissolve gelatin powder in a mixed solution of N,N-dimethylformamide: formic acid = 1:9 (v / v) to prepare a gelatin solution with a mass fraction of 30%; use a syringe to draw about 5mL of gelatin solution, insert a needle with a specification of 21G, and place it in a spinning machine; set the spinning parameters: distance 12cm, voltage 18kV. After spinning, the fiber membrane was removed and dried in an oven at 40° C. for 30 minutes. The surface of the fiber gelatin fiber is smooth and uniform in thickness, and the diameter of the fiber filament is about 200nm.

[0038] (2) Place the gelatin fiber membrane in saturated glutaraldehyde steam, cross-link for 24 hours at 25°C, take it out after cross-linking, place it in a fume hood for 30 minutes to volatilize the glutaraldehyde and dry it to obtain a degradable piezoelectric fiber scaffold. The fiber scaffold has Good piezoelectricity.

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Abstract

The invention belongs to the technical field of medical material preparation, and provides a preparation method of a degradable piezoelectric fiber stent and a product thereof. The preparation method of the degradable piezoelectric fiber stent comprises the following steps: preparing a gelatin solution, performing electrostatic spinning to prepare a fiber membrane, and drying to obtain a gelatin fiber membrane; placing the gelatin fiber membrane in glutaraldehyde saturated steam for normal-temperature crosslinking, and drying to obtain the degradable piezoelectric fiber stent. The preparation method provided by the invention adopts electrostatic spinning and chemical crosslinking modes for preparation, the method is simple, and reaction conditions are easy to realize. The prepared degradable piezoelectric fiber membrane stent can induce neural differentiation of neural stem cells in a wireless stimulation mode, and has important significance in clinical and nervous tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of medical material preparation, and in particular relates to a preparation method of a degradable piezoelectric fiber support and a product thereof. Background technique [0002] The spontaneous renewal and repair of the adult nervous system after traumatic injury or disease is limited, such as neurological dysfunction caused by cerebrovascular disease, brain tumor, traumatic brain injury, Parkinson's disease, demyelinating disease, etc. Current research has found that transplanting neural stem cells into damaged or injured brain tissue may generate new nerve cells and promote brain regeneration and repair. [0003] Neural stem cells (NSCs), as a multifunctional cell with self-renewal ability, have the potential to differentiate into neurons, astrocytes and oligodendrocytes, so NSCs can be induced to differentiate into functional Cells repair brain damage. In recent years, neural stem cell transplantation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/50D01D5/00
CPCA61L27/222A61L27/50D01D5/003A61L2430/32
Inventor 王金刚孙雅洁孙宝珺孟春霞刘宏
Owner UNIV OF JINAN
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