Method for preparing chitosan three-dimensional scaffold coated by graphene material

Abstract

The invention provides a method for preparing a chitosan three-dimensional scaffold having graphene oxide coatings of different reducing degrees. The method specifically comprises the following steps: preparing a chitosan scaffold material having a porous structure by a freeze drying method; then soaking the chitosan scaffold material in graphene oxide dispersion fluids of different concentrations; adsorbing graphene oxide sheets to pore walls of chitosan through the electrostatic interaction and the hydrogen-bond interaction; drying, and finally carrying out gradient heating and reducing to obtain the chitosan scaffold which has greatly improved mechanical strength and has graphene oxide coatings of different reducing degrees. The chitosan scaffold has a good application prospect in the aspect of neural tissue engineering. The method provided by the invention is simple to operate and can be used for preparing a great amount of desired product.

Description

Technical field: [0001] The invention relates to a method for preparing graphene-coated chitosan scaffolds with different reduction degrees. The invention utilizes the self-assembly principle of graphene oxide sheets and the electrostatic and hydrogen bonds between oxygen-containing functional groups and chitosan scaffold materials Function, realized the coating of graphene oxide on the chitosan scaffold, and finally obtained the composite scaffold of reduced graphene oxide and chitosan with tissue engineering application prospect through thermal reduction method. Background technique: [0002] Life and regeneration engineering, including the ability of humans to manipulate the nervous system, biological cells, tissues and organs in the future, is considered to be one of the disciplines involved in the sixth technological revolution in science and industry. Among them, regenerative technology, as an important technology of the fourth industrial revolution, has become the goa...

AI Technical Summary

Problems solved by technology

[0012] As a natural polycationic biopolysaccharide, chitosan exists in large quantities in nature and has good biocompatibility and biodegradability. The research on its use in tissue engineering is still in the ascendant, but as a bioactive scaffold, it faces Two main problems: (1) The lower mechanical properties are often difficult to support the growth of tissues and organs; (2) It is not easy to be modified, and it faces challenges in the controllable growth of cells

However, there is no report on the coating of graphene films with different reduction degrees on traditional polymer chitosan three-dimensional scaffolds by combining the simple impregnation method, chemical oxidation method, and low-temperature step heating reduction method.

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