Preparation method of mechanically-enhanced cell-loaded microchannel hydrogel
A hydrogel and microchannel technology, applied in the field of biomedical engineering, can solve the problems of cracking or collapse, easy blockage, affecting cell growth and functional expression, etc., to prevent shedding and invasion, good mechanical properties, and maintain stability. Effect
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Embodiment 1
[0017] A method for preparing a mechanically enhanced cell-carrying microchannel hydrogel, comprising the following steps:
[0018] The first step is to prepare a hydrogel sol. The photoinitiator, the hydrogel monomer and the hydrogel solvent are mixed uniformly in a mass ratio of 1:20:100 to prepare a hydrogel sol. The photoinitiator is 2-hydroxy- 2-methylpropiophenone, the hydrogel monomer is a mixture of agarose and polyethylene glycol dimethacrylate (PEG-DMA), the mass ratio of the mixture is 1:4, and the hydrogel solvent is a pH value of Phosphate (PBS) buffer solution of 5.7 to 8.0;
[0019] The second step is to prepare a mixture of hydrogel sol and cell culture solution, and the concentration of the hydrogel sol and cells prepared in the first step is 3 × 10 6 The cells / mL of the cell culture medium was mixed evenly at 40°C in a volume ratio of 1:1, and the cells in the cell culture medium were fibroblasts;
[0020] In the third step, the mixture of the hydrogel sol ...
Embodiment 2
[0026] A method for preparing a mechanically enhanced cell-carrying microchannel hydrogel, comprising the following steps:
[0027] The first step is to prepare a hydrogel sol. The photoinitiator, the hydrogel monomer and the hydrogel solvent are mixed uniformly in a mass ratio of 0.2:4:100 to form a hydrogel sol. The photoinitiator is Yanjiagu 2959 , the hydrogel monomer is a mixture of agarose and hyaluronic acid, the mass ratio of the mixture is 1:1, and the hydrogel solvent is a phosphate (PBS) buffer solution with a pH value of 5.7-8.0;
[0028] The second step is to prepare a mixture of hydrogel sol and cell culture solution, and the concentration of the hydrogel sol and cells prepared in the first step is 3 × 10 6 The cells / mL cell culture medium is mixed evenly at 40°C in a volume ratio of 1:1, and the cells in the cell culture medium are smooth muscle cells;
[0029] In the third step, the mixture of the hydrogel sol and the cell culture solution prepared in the seco...
Embodiment 3
[0035] A method for preparing a mechanically enhanced cell-carrying microchannel hydrogel, comprising the following steps:
[0036] The first step is to prepare a hydrogel sol. The photoinitiator, the hydrogel monomer and the hydrogel solvent are mixed uniformly in a mass ratio of 1:30:100 to form a hydrogel sol. The photoinitiator is Yanjiagu 2959 , the hydrogel monomer is polyethylene glycol diacrylate (PEG-DA), and the hydrogel solvent is a phosphate (PBS) buffer solution with a pH value of 5.7-8.0;
[0037] The second step is to prepare a mixture of hydrogel sol and cell culture solution, and the concentration of the hydrogel sol and cells prepared in the first step is 3 × 10 6 The cells / mL of the cell culture medium was mixed evenly at 40°C in a volume ratio of 1:1, and the cells in the cell culture medium were chondrocytes;
[0038] In the third step, the mixture of the hydrogel sol and the cell culture solution prepared in the second step is poured into a polymethyl me...
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