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10 ton liquid submerged femrentation culturing method of gleoesterum incarnatum

A technology of liquid submerged fermentation and gelatinous bacteria, applied in biochemical equipment and methods, bioreactor/fermenter combination, bioreactor/fermenter for specific purposes, etc., can solve the lack of large-scale industrial cultivation methods, etc. question

Inactive Publication Date: 2006-01-25
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention overcomes the defect of lack of large-scale industrialized cultivation methods in the current cultivation of Basidiogluceae colloides, and the technical problem solved by the present invention is to provide a large-scale (10 tons) liquid submerged culture method of Basidioglucetes colloides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 (10 tons of fermented liquid)

[0026] (1) Activation and cultivation of slant strains: transfer the preserved slant strains of Basidiomycetes colloides to the slant medium, cultivate at 25°C for 120 hours, and the mycelia cover the slant;

[0027] (2) First-level seed culture: pick a piece of the above-mentioned slanted strain of Bacterus colloides and insert it into a 500-milliliter shaker flask with 100 milliliters of medium to carry out first-level seed culture, culture conditions: rotary shaker 80 rpm Incubate at 25°C for 130 hours.

[0028] (3) secondary seed cultivation: the primary shake flask seed is inserted in the 500 milliliter shake flask that 100 milliliters of substratum is housed with 5% inoculum to carry out secondary seed cultivation, culture condition: rotary shaker 80 rpm , and cultivated at 25°C for 120 hours.

[0029] (4) Tertiary seed cultivation: the seeds of the secondary shaking flask are inserted into 5000 milliliters of shaking...

Embodiment 2

[0036] Embodiment 2 (10 tons of fermented liquid)

[0037] (1) Activation culture of slant strains: transfer the preserved slant strains of Basidiomycetes colloides to the slant medium, culture at 22° C. for 120 hours, and mycelium grows all over the slant.

[0038] (2) First-level seed culture: pick a piece of the above-mentioned slanted bacterial strain of Basidiomycetes colloides and insert it into a 500-milliliter shake flask equipped with 100 milliliters of medium to carry out first-level seed culture, culture conditions: rotary shaker 150 rpm Incubate at 25°C for 130 hours.

[0039] (3) Secondary seed cultivation: the secondary shake flask seed is inserted in the 500 milliliter shake flask that 100 milliliters of culture medium is housed with 10% inoculum and carries out secondary seed cultivation, culture condition: rotary shaker 100 rpm , and cultivated at 25°C for 100 hours.

[0040] (4) Tertiary seed cultivation: the seeds of the secondary shaking flask are inserted ...

Embodiment 3

[0047] Embodiment 3 (10 tons of fermented liquid)

[0048] (1) Activation and cultivation of slant strains: transfer the preserved slant strains of Basidiomycetes colloides to the slant medium, cultivate at 25°C for 100 hours, and the mycelia cover the slant;

[0049] (2) First-level seed culture: pick a piece of the above-mentioned slanted bacterial strain of Bacterus colloides and insert it into a 500-milliliter shake flask with 100 milliliters of medium to carry out first-level seed culture, culture conditions: rotary shaker 120 rpm Incubate at 25°C for 100 hours.

[0050] (3) Secondary seed culture: the first-level shake flask seed is inserted in the 500 milliliter shake flask that 100 milliliters of substratum are housed with 15% inoculum to carry out secondary seed cultivation, culture condition: 150 rpm of rotary shaker , and cultivated at 25°C for 96 hours.

[0051] (4) Tertiary seed cultivation: the seeds of the secondary shaking flask are inserted into 5000 millili...

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Abstract

The invention discloses a glue-tenacity leather bacterium 10 tons liquid deep layer fermenting cultivation method, which belongs to edible fungus fermenting field. The technology process includes the following steps: bevel fungus active cultivating, first class seed cultivating, second class seed cultivating, third class seed cultivating, first class seeding tank cultivating, second class seeding tank cultivating, and fermentor cultivating. The cultivating carbon source of seed and fermenting is glucose, cane sugar, and starch. Nitrogen source is peptone, defatted soybean albumen powder, bean cake powder, and ammonium sulfate. The inorganic salts need to be added is potassium di-hydrogen phosphate, magnesium sulfate. Vitamin need to be added is vitamin B1. The invention establishes a good foundation for developing and process glue-tenacity leather bacterium.

Description

technical field [0001] The invention belongs to the field of edible fungus fermentation industry, and in particular relates to a liquid submerged fermentation culture method for Basidiomycetes colloides. Background technique [0002] Gloeostereum incarnatum [Gloeostereum incarnatum], also known as elm ear and elm mill. Belongs to Basidiomycotina, Phytomycetes, Phyllomycetes, Pleurophyceae, and Glycotastella genus. Wild gum bacillus is mainly distributed in Northeast China and Hokkaido, Japan, Tieling, Qingyuan, Xinbin, Benxi and Fushun in Liaoning Province, Tonghua, Hunjiang, Fusong, Changbai and Antu in Jilin Province, and the eastern part of Heilongjiang Province The mountainous area is the main producing area of ​​elm, and a small amount is also distributed in Xinjiang. Bacillus colloides is a well-known fungus with both medicinal and edible functions. Ulmus has a long history of being introduced into traditional Chinese medicine. In the "Compendium of Materia Medica"...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
Inventor 蔡永峰岳国海李绩程池黄宇彤
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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