675results about How to "High regional selectivity" patented technology

PCT No. PCT / JP97 / 01387 Sec. 371 Date Dec. 11, 1997 Sec. 102(e) Date Dec. 11, 1997 PCT Filed Apr. 22, 1997 PCT Pub. No. WO97 / 40075 PCT Pub. Date Oct. 30, 1997A catalyst component for polyolefin production catalysts comprising a metallocene compound represented by general formula (1) (symbols have the meanings as described in the specification), polyolefin production catalyst containing the component, and method for producing polyolefin with the catalyst are provided. Use of a catalyst containing the novel metallocene compound as a catalyst component of the invention in polymerization of alpha -olefin, particularly propylene, enables one to prepare high rigid, high melting point isotactic polypropylene useful as an industrial material for automobiles and the like, more specifically isotactic polypropylene having highly controlled stereoregulartity and regioregularity, particularly the one having a high regioregularity that has been difficult to achieve with conventional metallocene catalysts.

The invention discloses a laser-reinforced jet-electrodeposition rapid-prototyping processing apparatus and method. An anode is connected with a laser generation mechanism, an eye shield is located between the laser generation mechanism and the anode, a cathode is a deposition substrate which is arranged in a deposition groove, and a power supply is connected with the cathode and the anode; a Z-axis mobile platform is connected with the anode and the laser generation mechanism, and an X-axis mobile platform and a Y-axis mobile platform are successively arranged below the deposition groove; the liquid inlet end of a constant temperature liquid storage mechanism is connected with the anode, the liquid returning end of the constant temperature liquid storage mechanism is connected with the deposition groove, and a controller is connected with the laser generation mechanism, the power supply, the X-axis mobile platform, the Y-axis mobile platform, the Z-axis mobile platform and the constant temperature liquid storage mechanism. According to the invention, a high-energy density laser beam and a high-speed electrodeposition solution are synchronously jetted to the surface of the cathode through a tubular passive anode centre bore, combination of laser reinforced electrodeposition technology and electrodeposition solution injection is realized, and a high deposition speed is obtained.

Provided are a saturated N-heterocyclic carbene-ligand metal complex derivative, a method for preparing the same, and a method for preparing a silane compound by hydrosilylation using the same as a catalyst. To describe in more detail, the metal complex derivative has a saturated N-heterocyclic carbene derivative and an olefin ligand at the same time. A silane compound is prepared by hydrosilylation in the presence of the metal complex derivative as a catalyst. The provided metal complex derivative of the present invention has superior stability during hydrosilylation reaction and is capable of effectively performing the hydrosilylation reaction at low temperature even with small quantity. Further, a product with superior regioselectivity may be obtained. In addition, after the hydrosilylation reaction is completed, the metal complex derivative may be recovered and recycled.

The invention discloses a recombinant nitrilase, a coding gene, a mutant and engineering bacteria from Acidovorax facilis ZJB09122, and the application thereof to preparation of 1-cyano cyclohexyl acetate. The invention provides a nitrilase and its mutant for hydrolysis of 1-cyano cyclohexyl acetonitrile, the enzyme and its mutant have good regional selectivity and catalytic activity in catalysis of the above reaction, and the production process is environmentally friendly. The invention solves the problems in a traditional chemical hydrolysis of 1-cyano cyclohexyl acetonitrile into 1-cyano cyclohexyl acetate, such as harsh reaction conditions, a large amount of organic solvent required in the reaction, high cost, low yield and serious environmental pollution.

The invention discloses a method for synthesizing tetrabenazine. The method comprises: step one, using a dimethylamine aqueous solution and a formaldehyde aqueous solution as initial materials to be reacted1 to obtain tetramethyl methane diamine; step two, dissolving the tetramethyl methane diamine obtained in the step one in an organic solvent, dropwise adding acetyl chloride and 5- methyl-2-hexanone, and performing amine methylation to obtain an intermediate 3-[(dimethyl amino) methyl]-5-methyl-2-hexanone; and step three, reacting the 3-[(dimethyl amino) methyl]-5-methyl-2-hexanone obtained in the step two with 6,7-dimethoxy-3,4-dihydroisoquinoline hydrochloride to obtain the tetrabenazine. According to the method for synthesizing tetrabenazine, the cheap and accessible materials are used as the initial materials, imine salts serve as amine methylation reagents to perform amine methylation reaction on the 5- methyl-2-hexanone, and accordingly, the region-selectivity of chemical reactions is improved; and water serves as a reaction solvent to prepare the tetrabenazine, so that the operation is simple and convenient, no complex post-processing process exists, and good industrial application prospects are provided.

A process for the carbonylation of a conjugated diene, which involves reacting the conjugated diene with carbon monoxide and a co-reactant having a mobile hydrogen atom in the presence of a catalyst system including: (a) a source of palladium; and (b) a bidentate diphosphine ligand of formula II, R1R2>P1—R3m—R—R4n—P2<R5R6   (II) wherein P1 and P2 represent phosphorus atoms; R1, R2, R5 and R6 independently represent the same or different optionally substituted organic radical containing a tertiary carbon atom through which each radical is linked to the phosphorus atom; R3 and R4 independently represent the same or different optionally substituted methylene groups; R represents an organic group having the bivalent bridging group C1—C2 through which R is connected to R3 and R4; m and n independently represent a natural number in the range of from 0 to 4, wherein the rotation about the bond between the carbon atoms C1 and C2 of the bridging group is restricted a temperature in the range of from 0° C. to 250° C., and wherein the dihedral angle between the plane occupied by the three atom sequence composed of C1, C2 and the atom directly bonded to C1 in the direction of P1, and the plane occupied by the three atom sequence C1, C2 and the atom directly bonded to C2 in the direction of P2, is in the range of from 0 to 120°; and (c) a source of an anion.

The present invention relates to novel monooxygenase nucleic acids and polypeptides created using mutagenesis, DNA shuffling, or both, in a single iteration or multiple iterations, and methods for their creation and use. The monooxygenase enzymes of the present disclosure have particular utility as biocatalysts in industrial chemical redox reactions, such as the oxidation of aromatic hydrocarbons, for example, toluene, benzene, or nitrobenzene, into industrially desirable products. The systems and processes of the present invention are especially useful for the coupled synthesis and recovery of catechols, methylcatechols, resorcinols, methylresorcinols, hydroquinones, methylhydroquinones, hydroxybenzenes, cresols, nitrobenzenes, and nitrohydroxyquinones.

The invention discloses a method for preparing artemisinin through arteannuic acid. The method comprises the steps that first the arteannuic acid is processed to obtain a dihydroartemisinic acid under the effect of a reducing agent such as sodium borohydride / nickel chloride or a hydrogen / metal catalyst, and then the dihydroartemisinic acid is oxidized into a peroxided dihydroartemisinic acid through peroxide in the presence of the catalyst, and finally the target product artemisinin can be obtained with high yield under the catalyzing of the acid and the effect of oxygen; or a dihydroartemisinic acid derivative can be obtained from the dihydroartemisinic acid based on the protection on carboxyl, and the dihydroartemisinic acid derivative is oxidized into a relevant peroxided dihydroartemisinic acid derivative through the peroxide in the presence of the catalyst, and then the target product artemisinin can be obtained with high yield under the catalyzing of the acid and the effect of the oxygen. Compared with the prior art, the method for preparing the artemisinin through the arteannuic acid has the advantages as follows: the used agent has low cost, and is easy to obtain; the synthetic route is short; the reaction selectivity is high; the preparation process is environmental-friendly; the operation and post-processing are simple; the total yield is high; and the method for preparing artemisinin through the arteannuic acid is applied to industrial production.

The invention relates to a traditional Chinese medicine fermented preparation for improving production performance of sows, and belongs to the technical field of preparation for livestock. The preparation comprises the following traditional Chinese medicine components in parts by weight: 10-20 parts of astragali roots, 10-20 parts of codonopsis roots, 5-15 parts of large-headed atractylodes rhizomes, 5-15 parts of Chinese angelica, 10-15 parts of bupleurum roots, 5-10 parts of dried orange peels, 5-10 parts of cimicifuga roots, and 10-20 parts of licorice. The traditional Chinese medicine components selected by the method has the advantages of reasonable structure and proper proportion, selected Lactobacillus plantarum has the advantages of high activity, good stress resistance and the like; in the effects of the selected Lactobacillus plantarum, and efficacies of the original medicines are substantially improved; the fermented medicine can substantially improve production performance of sows, and is green and healthy without toxic and side effects.

The invention belongs to the technical field of medicine chemical engineering synthesis, and discloses a synthetic method of fluorine-containing alkyl substituted 2,3-dihydrocoumarone derivatives and indole derivatives. The synthetic method comprises the following steps: stirring and reacting raw materials comprising a 2-allylphenol or a 2-allylaniline compound and an iodine-substituted fluorine-containing reagent in nitrogen atmosphere at 60-100 DEG C for 12-24 h with palladium as a catalyst, phosphine as a ligand, an alkali as an additive and an organic solvent as a solvent, cooling the obtained reaction solution to room temperature after the above reaction ends, carrying out extraction on the cooled reaction solution with ethyl acetate, carrying out reduced pressure solvent removal to obtain a crude product, and carrying out column chromatography purification to obtain products, wherein the equation of the reaction is represented by formula (I) shown in the description. The synthetic method for synthesizing the fluorine-containing alkyl substituted 2,3-dihydrocoumarone derivatives and indole derivatives in a one-step manner has the advantages of simplicity, easiness in implementation, mild reaction conditions, wide adaptability to a substrate, high product yield, and good industrial application prospect.

The invention provides a cheap and efficient microorganism enzyme producing bacteria, namely, bacillus cereus (Bacillus cereus) WZZoo1, and provides a method for preparing (S)-alpha-ethyl-2-oxyen-1-pyrrolidine acetic acid ester by taking racemic alpha-ethyl-2-oxygen-1-pyrrolidine acetic acid ester as reaction substrate and catalyzing stereoselectivity hydrolysis(R)-alpha-ethyl-2-oxygen-1-pyrrolidine acetic acid ester through carboxylic ester hydrolase generated by the bacterium. The bacterial strain is preserved in China Center For Type Culture Collection (CCTCC) on October 14, 2012, the preservation serial number is CCTCC No: M2012403. The beneficial effect of the method mainly reflects in that the carboxylic ester hydrolase generated by the bacterial strain has strong area selectivity and high reaction conversion rate, downstream separation is simple, energy consumption is low, and environment pollution is light, so that the method is applicable to industrial production.

The invention discloses recombinant transaminase as well as a preparation method and application of the recombinant transaminase. An amino acid sequence of a high-activity recombinant transaminase mutant is as shown in SEQ ID NO.2 and a coding gene is as shown in SEQ ID NO.1. The method for preparing the recombinant transaminase compries the steps of fermenting and cultivating a gene engineering bacterium containing the coding gene, and collecting and preparing the recombinant transaminase. The recombinant transaminase is applied to asymmetric synthesis of a chiral amine compound, and particularly is applied to synthesis of a sitagliptin midbody (R)-3-amino-4-(2,4,5-trifluoromethyl phenyl)-methyl butyrate. The related transaminase has excellent stereoselectivity, regioselectivity and catalytic activity, the catalytic reaction is mild, the reaction conversion rate and the yield of a product are high, and the recombinant transaminase has relatively good application prospect.

The invention belongs to the field of drug synthesis, and relates to a method for synthesizing a second-generation taxol anticancer drugs Cabazitaxel XRP6258. The method comprises the following steps of: obtaining C-7 and C-10 bit hydroxy methyl mercaptan methylene (MTM) and C-13 bit oxhydryl oxydic key intermediate (II) of 10-oxhydryl baccatin III(I) by high regioselectivity, reducing C-13 bit carbonyl to obtain a mother nucleus midbody (III), carrying out butt joint with various types of side chains to obtain butt joint product midbodies (IV-1) and (IV-2), and removing a side-chain protecting group after the di-methylthio is removed or removing the di-methylthio of the nuclear parent after the side-chain protecting group is removed to obtain a product Cabazitaxel XRP6258 (V). The method disclosed by the invention has the advantages of being simple in preparation process, high in yield, lower in cost, easy to operate and the like, so that the XRP6258 can be produced and prepared on a large scale.

The invention discloses a method for preparing a nitro compound from a low-valent nitric oxide. The method comprises that a low-valent nitric oxide such as nitric oxide, nitrogen dioxide, nitrous oxide, nitrogen trioxide, dinitrogen tetroxide and the like react with an alicyclic compound, an aromatic compound or a heterocyclic compound through promoting effects of molecular oxygen and effects of catalysts comprising a transition metal oxide, a triphenylphosphine metal organic complex, transition metal ion clay and heteropoly acid salts such as aluminosilicate, silicoaluminophosphate, sodium phosphomolybdate and the like. The method can replace the traditional industrial method for preparing a nitro compound from a high-valent (V-valent) nitrogen compound such as nitrogen pentoxide, hydrogen nitrate, nitric acid salts, nitric acid esters and the like. The method improves an atom utilization rate of a reaction in industrial nitration preparation of a nitro compound, and has atom economic characteristics of industrial preparation of a nitro compound.

The invention discloses a benzimidazole chiral heterocyclic compound as well as a preparation method and application thereof. The structure formula of the benzimidazole chiral heterocyclic compound isshown in formula I, formula II or formula III in the description. The benzimidazole chiral heterocyclic compound is prepared by treating an iridium complex which is prepared through a metal iridium compound and a phosphoramidite ligand as a catalyst, performing intra-molecular allyl amination on allyl substrate, and then synthesizing with high efficiency and high enantioselectivity. The method has the advantages of being high in catalytic reaction activity, mild in reaction conditions, high in enantioselectivity, wide in substrate applicable scope, and environmentally friendly; the primary in-vitro enzyme inhibition activity test shows that the compound is high in alpha-glucosidase inhibiting activity and can be used as an alpha-glucosidase inhibiting agent, and the compound can also be used as a further modified drug intermediate; the compound has a potential application value in preventing or treating II-diabetes, obesity and complication medicines thereof and pilot compounds thereof.

Processes for the production of chlorinated alkanes are provided. The present processes comprise reacting one or more mono- and / or dichloroalkanes to form tri-, tetra- and / or pentachloroalkanes, with high regioselectivity. In those embodiments wherein a dichloroalkane is desirably utilized, it may advantageously be a vicinal dichloroalkane. Further, only one catalyst is utilized. The present processes make use of sulfuryl chloride as a chlorinating agent, rather than a gaseous chlorinating agent such as chlorine gas. Finally, the process uses lower intensity process conditions than at least some conventional processes, and thus, operating costs are saved.

The invention provides a method and device for forming a half-mold of a metal flow field plate of a mini-type fuel cell in a laser shock mode. The method comprises the steps that according to the designed shape and the designed size of a flow channel of the metal flow field plate, a template provided with a groove with the same shape and the same size is machined, and a motion path of laser beams is well planned according to the shape and the size of the flow channel; a control system controls the motion direction, the path and the machining speed of the laser beams according to a track and a whole flow field is formed through accumulation of plastic forming. The device for forming the half-mold comprises a workbench base, a blank pressing device, the control system and a forming system. According to the method and device, the snake-shaped metal flow field plate can be formed, machining is simple, accuracy and efficiency are high, cost is low, and popularization and application of the metal flow field plate of the fuel cell and a mini-type PEMFC are facilitated.

The invention discloses a process for continually preparing valeraldehyde, which reacts 1-butene raw material continually containing a little of trans / cis-2-butene, isobutene, butane with synthesis gas in the presence of the organic phosphinerhodium catalyst and triphenylphosphine. The process of the invention includes a reaction region, separation region and an activation region. Butene hydroformylation reaction liquid is evaporated, condensed and separated to two streams of raw product and circulating catalyst solution by film evaporation equipment, under shielding gas atmosphere of synthesis gas, and activated circulating catalyst solution enters reactor again. The present invention activates rhodium catalyst by catalyst activator, and controls or reduces acetal high boiling point byproducts which are generated by aldehyde polycondensation, enhance catalyst activity and regioselectivity of aldehyde. The film evaporation equipment used in the present invention is more effective than flash evaporator to separate catalyst solution and valeraldehyde producers in reaction liquor. Appropriate operational condition of film evaporation equipment can reduce or not generate aldehyde condensation, protive rhodium catalyst, enhance catalyst activity and prolong usage time of catalyst.

The invention discloses a temperature-sensitive platinum catalyst and a preparation method thereof. For preparing the temperature-sensitive platinum catalyst, a nanophase material with large specific surface is used as a carrier; the adsorption of the nanophase material is utilized to adsorb a platinum complex on the surface of the nanophase material; the platinum complex is made stable through the heat treatment, then a layer of temperature-sensitive resin is coated on the surface of the platinum complex, and a temperature-sensitive platinum catalyst powder is obtained through the dry treatment or a temperature-sensitive platinum catalyst colloid is obtained by dispersing the temperature-sensitive platinum catalyst powder into a liquid phase system. The temperature-sensitive platinum catalyst has the following obvious characteristics that the catalyst is very stable under the softening point temperature of the temperature-sensitive resin and hardly has catalysis effect, but immediately has high catalytic activity above the softening point temperature so as to achieve the purpose of fast curing. The temperature-sensitive platinum catalyst is suitable for the curing system of addition-type silicon rubber and silicone at medium and high temperature.

The invention discloses a molecular sieve encapsulated rhodium heterogeneous core-shell catalyst for olefin hydroformylation, and a catalytic method thereof, and belongs to the field of novel catalysis materials. Submicron Silicalite-1 (S-1) zeolite molecular sieve grain supported rhodium ions are used as a crystal seed to induce epitaxial growth of an S-1 shell, a ruthenium catalytically active species is encapsulated in the S-1 zeolite molecular sieve to form the core-shell Rh@S-1 catalyst, and the catalyst is used in a heterogeneous hydroformylation reaction to synthesize normal aldehydes.The olefin conversion rate and the aldehyde yield are respectively more than 95%, a normal-to-iso ratio of the hydroformylation products is 1.1-2.0, and the catalyst can be recycled. Compared with homogeneous catalyst system industrially applied at present, the catalyst in the invention solves the problem of high energy consumption needed by separation of the catalyst and the hydroformylation products, and avoids the degradation abuse of the catalytically active component due to the high temperature rectification process during separation of the catalyst, so the recycling of the catalyst is benefited, and the catalyst has a broad application prospect.

The invention discloses an NNN ligand, metal complexes thereof, preparation methods and application, and particularly provides an NNN ligand 1, a metal complex 2 of the NNN ligand, a metal complex 3 of the NNN ligand and preparation methods of the NNN ligand 1 and the metal complexes, as well as application of the metal complex 3 of the NNN ligand to catalysis on hydroboration reaction of bis-substituted olefin, especially application of the metal complex 3 to catalysis on asymmetric hydroboration reaction of 1, 1-bis-substituted olefin. The metal complex 3 of the NNN ligand has good catalytic activity in the hydroboration reaction of the bis-substituted olefin, especially asymmetric hydroboration reaction of the 1, 1-bis-substituted olefin, has excellent regioselectivity and enantioselectivity, and is high in yield and mild in reaction conditions; besides, the preparation methods of the NNN ligand and the metal complexes thereof are simple, environment-friendly, mild in reaction conditions, relatively high in yield, and simple in post-processing, and the raw materials are low in cost and easy to obtain, so that the preparation methods are suitable for industrial production. (The formulas of the NNN ligand 1, metal complex 2 and metal complex 3 are shown in the description).

The invention discloses a preparation method of a pyrimidine nucleoside compound shown in a formula 1 or a purine nucleoside compound shown in a formula 2, comprising the following steps of: performing glucosidation on a compound 3 and a compound 4 to obtain the pyrimidine nucleoside compound 1; or performing glucosidation on the compound 3 and a compound 5 to obtain the purine nucleoside compound 2. The preparation method disclosed by the invention is applicable to preparation of many kinds of nucleoside compounds, and has the advantages of mild reaction conditions, greenness, environmental friendliness and higher yield and purity of the product.

A method for enzymatic condensation polymerization by combining a preselected quantity of an enzyme, a diol and / or a polyol, and a diacid in a reaction vessel; heating the reaction vessel containing the enzyme, the diol or polyol, and the diacid to a preselected temperature; and maintaining the reaction vessel containing the enzyme, the diol and / or polyol, and the diacid at the preselected temperature for a preselected time, thereby producing a condensation polymer.

The invention relates to a preparation method of 5-methyl isoxazole-4-ethyl formate, characterized by using cheap and easily available ethyl acetoacetate and triethyl orthoformate as raw materials and condensing the raw materials to prepare 2-ethoxymethylene ethyl acetoacetate, then reacting 2-ethoxymethylene ethyl acetoacetate with an aqueous solution of hydroxylamine hydrochloride and inorganic base in an organic solvent to prepare 5-methyl isoxazole-4-ethyl formate, wherein the total yield of the two steps is higher than 78.0%, the content is higher than 99.0%, and the content of the isomer 3-methyl isoxazole-4-ethyl formate is less than 1.0%. The preparation method has the advantages of low production cost, low content of the isomer 3-methyl isoxazole-4-ethyl formate, simple process, and mild reaction conditions, and is suitable for industrial production.

The invention provides a novel bacterial strain-Yokenella sp. WZY002 and an application thereof in preparing alpha, beta-unsaturated olefine aldehyde (ketone) through regioselective reduction and aromatic alcohol through aromatic aldehyde (ketone) reduction. The bacterial strain is preserved in the China general microbiological culture collection center (CCTCC); the address is 430072, Wuhan University, Wuhan, China; the preservation number is CCTCC No: M2013099; the preservation date is March 22, 2013. The novel bacterial strain has the main beneficial effects of high regioselectivity, high stereoselectivity and high enzymatic activity, regioselective reduction of the alpha, beta-unsaturated enol is catalyzed so as to obtain various alpha, beta-unsaturated olefine aldehydes, and reduction of the aromatic aldehyde (ketone) can also be catalyze so as to obtain the aromatic alcohol. The bacterial strain is served as a biocatalyst and has high regioselectivity, high stereoselectivity and strong catalytic activity, the catalytic reaction does not need to add coenzyme, the reaction temperature is moderate, and the novel bacterial strain has a relatively high application value for industrial production.

The invention discloses a synthesizing method of a butyrolactone derivative. The synthesizing method comprises the following steps of (1) activating (R)-2-propyl-ethylene oxide shown in a formula (II)by a titanium reagent, and performing addition reaction by an organic zinc compound, so as to obtain a compound shown in a formula (III); (2) performing molecular lactone exchange reaction on the compound shown in the formula (III) under the acid condition, so as to obtain the butyrolactone derivative shown in a formula (I). The synthesizing method has the advantages that the synthesizing steps are simple, the production cost is low, the selectivity of areas is strong, and the yield rate is high; the synthesizing route is shown in the description.

The invention provides spiro bis(oxazoline) ligands with a plurality of chiral centers, a preparation method and application. The ligands are provided with axial chirality of a spiro backbone and central chirality of an oxazoline ring. The ligands can be prepared by condensation of chiral spiro diacid and corresponding alkamine. The invention also provides application of the spiro bis(oxazoline) ligands in synthesizing pyrazolidine derivatives in high regioselectivity and high enantioselectivity.

Belonging to the technical field of biology, the invention particularly relates to a transaminase mutant, a construction method and application thereof. The transaminase mutant provided by the invention comprises a variety of mutants subjected to single-point mutation and combined mutation at the 259th site, 431st site, 264th site, 269th site and 381st site in an amino acid sequence of wild transaminase (WP_0532423951.1), and compared with the wild type transaminase (WP_0532423951.1), the mutant has a longer half-life period at 55DEG C, the half-life period of an optimal combined mutant is about 5 times that of the wild type transaminase (WP_0532423951.1), the thermal stability is better, and the mutant is suitable for catalytic synthesis of chiral amine at high temperature. The transaminase mutant obtained by the construction method provided by the invention shows excellent stereoselectivity and catalytic activity when used for catalytic synthesis of chiral amine at high temperature,and has good application prospects.

The invention discloses a ring opening method of aziridine compounds, particularly a ring opening method of aziridine compounds in different structures by using metal bromide, belonging to the technical field of organic synthesis. The aziridine compound is subjected to ring opening reaction by using tosyl-activated aziridine compounds as the initial raw material, dichloromethane as a solvent and bromine in the metal bromide as a nucleophilic reagent. The method has the advantages of simple reaction process, mild conditions and wide applicability, can obtain higher yield for aziridine compounds in different structures, has very high regioselectivity, and can form a single isomer from most aziridine compounds.