The invention discloses a method for extracting 10-deacetyl
baccatin III from
Taxus chinensis var.mairei, and relates to the technical field of
natural product extraction. The method comprises the following steps: (1) crushing is conducted,
liquid nitrogen treatment is conducted, and
ammonia gas blasting treatment is conducted; (2)
petroleum ether is subjected to ultrasonic
impurity removal; (3)
ethanol is subjected to high-pressure and constant-temperature extraction and ultrasonic extraction; (4) decompression concentration is conducted,
dissolution is conducted though
acetone, filtering andconcentration are conducted, and thus a crude extract I is obtained; (5) macroporous adsorption resin is washed though an alkali solution and deionized water in sequence and then eluted with the
ethanol, eluent is collected and concentrated, and thus a crude extract II is obtained; (6) primary recrystallization is conducted, specifically, recrystallization is conducted through
acetonitrile; and (7) secondary recrystallization is conducted, specifically, recrystallization is conducted through an
acetone /
petroleum ether system. The method is high in
operability, the steps are reasonably set, operation parameters are strictly controlled, the 10-deacetyl
baccatin III with the excellent purity and yield can be obtained, the purity reaches 99.3% or above, and the yield is 0.0242% or above.