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Method for elementary separating 10-noracetyl Baccatins III from branches and leaves of yew

A technology for deacetylation of yew branches and leaves, which is applied in the field of extraction of yew plant components, can solve the problems of high cost, poor safety, and high energy consumption, and achieve the effects of high recovery rate, high safety, and reduced usage

Active Publication Date: 2005-08-31
GUANGDONG KELUN PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the former eliminates the need for organic solvent extraction, the spray drying method is used to concentrate and dry a large amount of water extract, which consumes a lot of energy and costs high; the latter uses a large amount of organic solvent for extraction in the initial separation, which is costly and poor in safety.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] In four 250ml glass conical flasks, add respectively the filtrate of the concentrated solution of the 70% ethanol extract of 13.13ml yew branches and leaves, contain 4.11mg10-DABIII in the filtrate, be diluted with distilled water 16.87ml to be 30ml respectively, pH value 5; Add 5g each of the treated wet-based macroporous adsorption resins S-8, D4020, X-5, and D3520, and the dry weights are 1.25g, 1.1g, 1.35g, and 1.1g, respectively.

[0015] Place the above four test sample bottles at 20°C for 4 hours on a shaker, shake the shaker at a speed of 103 rpm, filter in a glass funnel with ordinary qualitative filter paper, and obtain a macroporous adsorption resin that has adsorbed 10-DABIII and filtrate. Measure the content of residual 10-DABIII in the filtrate with HPLC, calculate the adsorption capacity of every kind of macroporous adsorbent resin; Adsorbed the macroporous adsorbent resin of 10-DABIII is packed in the Erlenmeyer flask respectively, then respectively add ...

Embodiment 2

[0022] Get two parts of the filtrate of the concentrated solution of the 80% ethanol extract of yew branches and leaves prepared in two parts of each 6.1ml, contain 10-DABIII 1.91mg in the filtrate, add respectively in the 50ml glass conical flask, add distilled water 8.9ml to be diluted to 15ml, measure The pH value is 5.2. Weigh 5g each of the treated wet-base macroporous resins AB-8 and NKA-9, and the dry weights are 1.38g and 1.58g respectively; put them into two 35cm×0.7cm glass chromatography columns, and use distilled water After equilibrating, pour the prepared two parts of filtrate containing 10-DABIII into two chromatography columns respectively, open the lower piston of the column, and let the filtrate slowly flow through the macroporous adsorption resin in the column, and repeat 3 times to make the filtrate 10-DABIII in was completely adsorbed. After detecting that the effluent from the chromatography column does not contain 10-DABIII by HPLC, the effluent was dis...

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PUM

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Abstract

A process for primarily extracting 10-deacetyl palcatine C from the twig and leaf of enqlish yew includes extracting in alcohol solution, concentrating laying aside, deposing taxol and low-polarity impurities, filtering, regulating pH=5-6, macroreticular resin adsorbing, eluting with absolute alcohol, and concentrating.

Description

technical field [0001] The invention relates to a method for extracting plant components of yew, in particular to a method for primary separation of 10-deacetylbaccatin III from branches and leaves of yew with macroporous adsorption resin. Background technique [0002] 10-Deacetylbaccatin III (10-DABIII) is a homologue of the anticancer drug Taxol (Taxol), and is also a precursor component of Taxol synthesized in plants. Now it is used as the precursor raw material of artificial semi-synthetic paclitaxel or docetaxel. Paclitaxel or docetaxel has been clinically used in the treatment of breast cancer, ovarian cancer, lung cancer, etc. It has good curative effect and small side effects, and is a newcomer in today's anticancer drugs. Our country and many countries in the world have approved production and clinical use, and the demand is increasing day by day. In the past, taxol was mainly derived from the bark of natural yew, and yew plants are rare and protected plants in th...

Claims

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Application Information

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IPC IPC(8): C07D305/14
Inventor 王国亮张依健骆雪兰黄巧明谢维权夏惠青王彬力曾秋玲叶瑞英侯嵩生李志良
Owner GUANGDONG KELUN PHARMACEUTICAL CO LTD
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