383 results about "Chiral amine" patented technology

The invention relates to an asymmetric synthesis method of (S,S)-2,8-diazabicyclo[4,3,0] nonane (I) as a chiral intermediate of moxifloxacin. The asymmetric synthesis method comprises the steps of: firstly, carrying out a dewatering reaction on a 3-pyrrolidone compound (shown as formula III or IV) and chiral amine, reducing a dewatered product to obtain enantiopure compounds shown as a formula (II) under different reducing conditions, and carrying out intramolecularly cyclization and removal of chiral prothetic groups on the compounds shown as the formula (II) to obtain (S,S)-2,8-diazabicyclo[4, 3, 0] nonane (I). The invention also relates to 3-pyrrolidone shown as a formula (III) or (IV) and a preparation method thereof, wherein R is an amino protection group; in the formula (II), * is a chiral center mark of the chiral prothetic groups, R1 and R2 are respectively C1-4 alkyl, C1-4 hydroxyl or carboxyl substituted C1-4 alkyl, aryl, carboxyl, C1-4 carbalkoxy or carbamoyl, when Z is H2, Y is chlorine, bromine, iodine or hydroxyl sulphonate, when Z is O, Y is hydrogen, hydroxyl or C1-4 alkoxy; in the formula (III), R3 is hydrogen or C1-4 alkyl; and in the formula (IV), Y is chlorine, bromine, iodine or hydroxyl sulphonate.

The invention discloses a method for preparing an optically active carbonyl compound, which comprises the following step: under catalysis of a chiral amine salt and a transition metal catalyst, carrying out asymmetric catalytic hydrogenation reaction on alpha, beta-unsaturated aldehyde or alpha, beta-unsaturated ketone compound by taking hydrogen gas and a catalysis amount of dihydropyridine compound as a hydrogen source, so as to obtain the optically active carbonyl compound. The method has mild reaction conditions and simple operation, and the use amount of the dihydropyridine compound is the catalysis amount, so that a target product is easily separated and purified from a reaction system; and at the same time, the metal catalyst can be recycled, thereby meeting the economy requirement.

The invention provides an amino acid sequence, a preparation method and a reaction process of engineered transaminase for asymmetric synthesis of chiral amine compounds under industrial-related conditions. The invention also provides a polynucleotide sequence encoding the engineered transaminase polypeptide, recombinant engineering bacteria capable of expressing the engineered transaminase polypeptide, and a method for producing the chiral amine compounds by using the engineered transaminase polypeptide. Compared with other preparation methods, the engineering transaminase polypeptide providedby the invention has better activity and stability, overcomes the inhibition of the product on enzymes, has higher unit activity, and has good industrial application prospect.