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Method for preparing triple inactivated vaccine for preventing chicken Newcastle disease, infectious bronchitis and egg drop syndrome

A technology of bronchitis and inactivated vaccines, applied in the field of vaccines to prevent poultry infectious diseases, can solve the problems of chicken industry hazards, inconvenient use, high cost of epidemic prevention, etc., to reduce the cost of epidemic prevention, reduce the number of injections, and have good safety Effect

Inactive Publication Date: 2008-01-09
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0002] Chicken Newcastle disease, infectious bronchitis M 41 Strains (respiratory and HN 99 Kidney-type bivalent) and egg production drop syndrome are very harmful to the poultry industry. At present, there are only corresponding single inactivated vaccines, such as chicken Newcastle disease inactivated vaccine, infectious bronchitis (monovalent) inactivated vaccine, Egg drop syndrome vaccine needs to be injected separately, the procedures are cumbersome and inconvenient to use, and the cost of epidemic prevention is high

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0047] 6. Preparation and inspection of virus liquid for seedling production

[0048] (1) Preparation and inspection of NDV LaSota strain virus solution Same as items 2.2, 2.3, and 2.4 of "Manufacturing and Inspection Procedures for Chicken Newcastle Disease Low Virulence Live Vaccine" in "Procedures".

[0049] (2) IBV M 41 strain and HN 99 Preparation and testing of strain virus liquid

[0050] Materials for making seedlings Select well-developed 10-11-day-old susceptible chicken embryos as materials for making seedlings.

[0051] Chick embryo inoculation M 41 strain and HN 99 Viruses were diluted with sterilized saline to 10 -2 ~10 -3 , respectively inoculate the allantoic cavity of susceptible chicken embryos, 0.1ml per embryo, seal the pinholes after inoculation, continue incubation at 37°C, and stop turning eggs.

[0052] Incubation and observation After inoculation of chicken embryos, eggs should be illuminated once 30 hours before, dead embryos should be discarde...

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Abstract

The invention is concerned with a kind of preparation method of inactivated vaccine to prevent new castle disease, infectious bronchitis and egg drop syndrome vaccine. The selected genus is Newcastle Disease virus La Sota individual plant, infectious bronchitis virus M41 individual plant, HN99 kidney type of aberrance individual plant and EDS76 Jing 911 individual plant. Dilute three kinds of virus and inoculate in the allantoic cavity of SPF chick embryo and affectable duck embryo. Collect liquid of embryo to alive and dead embryos as seed. Dilute and inoculate in allantoic cavity of affectable chick embryo or duck embryo to get liquid of virus embryo. Concentrate with equipment and add with formaldehyde solution to prepare bacterin through emulsion process. This invention can prevent Newcastle Disease, chick kidney type and breathing type infectious bronchitis, and egg drop syndrome vaccine. It can reduce the times of injection and the cost of epidemic prevention for easy using and practicality.

Description

technical field [0001] The invention belongs to a vaccine for preventing poultry infectious diseases, in particular to a preparation method of a triple inactivated vaccine for preventing Newcastle disease, infectious bronchitis and egg production drop syndrome. Background technique [0002] Chicken Newcastle disease, infectious bronchitis M 41 Strains (respiratory and HN 99 Kidney-type bivalent) and egg production drop syndrome are very harmful to the poultry industry. At present, there are only corresponding single inactivated vaccines, such as chicken Newcastle disease inactivated vaccine, infectious bronchitis (monovalent) inactivated vaccine, Egg drop syndrome vaccine needs to be injected separately, the procedures are cumbersome and inconvenient to use, and the cost of epidemic prevention is high. Contents of the invention [0003] The object of the present invention is to provide a preparation method of a triple inactivated vaccine for preventing four kinds of infe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215A61K39/12A61P11/00
Inventor 崔保安舒德广王学斌魏战勇徐端红王莉高文明张素梅
Owner HENAN AGRICULTURAL UNIVERSITY
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