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Porcine epidemic diarrhea and porcine parvovirus bivalent vaccine and application thereof

A porcine epidemic diarrhea and parvovirus technology, applied in the direction of viruses, vaccines, antiviral agents, etc., can solve the problems of weak resistance and death of piglets, and achieve the effects of improving protection, ensuring calving ability, and strong immunogenicity

Active Publication Date: 2021-06-01
WUHAN KEQIAN BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, there are many related single vaccines on the market, all of which only target one of the diseases - porcine diarrhea or porcine reproductive disorder; due to the weak resistance of newborn piglets, the situation of stress is particularly prominent when they are immunized with vaccines. Severe cases can lead to death of piglets

Method used

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  • Porcine epidemic diarrhea and porcine parvovirus bivalent vaccine and application thereof
  • Porcine epidemic diarrhea and porcine parvovirus bivalent vaccine and application thereof
  • Porcine epidemic diarrhea and porcine parvovirus bivalent vaccine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of Porcine Epidemic Diarrhea Virus Variant 2a Virus Liquid

[0029] (1) Propagation of poisonous seeds for production

[0030] Including cell expansion, virus inoculation, virus liquid harvest, virus titer determination, sterility and exogenous detection.

[0031] 1. Cell expansion culture: After calibrating the dissolved oxygen (DO) electrode, PH electrode, and temperature electrode of the bioreactor, sterilize it by autoclaving, and pump it into the growth of 20% of the total volume of the bioreactor for use. The density of suspended ST cells in the shaker flask reaches 7-10×10 6 When cells / mL and the cell viability is more than 95%, the suspension-type ST cells are grown at 0.5-1×10 6 The density of cells / ml was transferred to the bioreactor, and the optimal culture conditions were set as 37°C, PH 7.2-7.4, DO 40%-60%, 70r±5.

[0032] 2. Virus inoculation: When the ST cell density in the bioreactor reaches 7-10×10 6 cells / mL, dilute ST cells ...

Embodiment 2

[0049] Example 2 Preparation of porcine parvovirus CH01 strain virus liquid

[0050] (1) Propagation of poisonous seeds for production

[0051] 1. Including cell expansion, virus inoculation, virus liquid harvest, virus content determination, sterility and exogenous detection.

[0052] 2. Cell expansion culture: After calibrating the dissolved oxygen (DO) electrode, PH electrode, and temperature electrode of the bioreactor, and autoclaving it, pump it into the bioreactor to grow 20% of the total volume for use. The density of suspended ST cells in the shaker flask reaches 7-10×10 6 When cells / ml and the cell viability is more than 95%, the suspension type ST cells are plated at 0.5-1×10 6 The density of cells / mL was transferred to the bioreactor, and the optimal culture conditions were set to 37°C, PH 7.2-7.4, DO 40%-60%, 70r±5.

[0053] 3. Virus inoculation: until the ST cell density in the bioreactor reaches 7-10×10 6 cells / mL, dilute ST cells with growth medium to a den...

Embodiment 3

[0071] Example 3 Preparation and inspection of porcine epidemic diarrhea and porcine parvovirus dual vaccine

[0072] (1) Preparation of PEDV / PPV dual inactivated vaccine

[0073] 1. Aqueous phase preparation: Mix the inactivated porcine epidemic diarrhea virus variant strain 2a and porcine parvovirus CH01 strain virus liquid in a volume ratio of 1:1 evenly.

[0074] 2. Emulsification and sub-packaging: Mix the water phase with 201 adjuvant 1:1 by volume and then emulsify, quantitatively sub-pack, capping, and labeling after passing the test; the content of porcine epidemic diarrhea virus variant 2a is greater than or equal to 10 7.3 TCID 50 / mL, the content of porcine parvovirus CH01 strain ≥ 10 7.5 TCID 50 / mL.

[0075] 3. Vaccine testing methods and results

[0076] Prepare 3 batches of vaccines according to the above method, the batch numbers are: 20200301, 10190302, 20200303

[0077] (1) The appearance of 3 batches of inactivated vaccines is milky white

[0078] (2...

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Abstract

The invention relates to a porcine epidemic diarrhea and porcine parvovirus bivalent vaccine. The porcine parvovirus CH01 strain with the preservation number of CCTCC NO: V202085 and the porcine epidemic diarrhea virus variant 2a are inoculated into suspended ST cells, the bivalent vaccine is prepared through a full suspension method, and the obtained bivalent vaccine is high in immunogenicity, has high protection force on the sow and the farrowing capacity of the sow and can effectively guarantee normal reproduction of the sow. The bivalent vaccine provided by the invention has important significance in the field of common immunization of the porcine epidemic diarrhea virus and the porcine parvovirus.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a dual vaccine of porcine epidemic diarrhea and porcine parvovirus and its application. Background technique [0002] Porcine epidemic diarrhea virus (PEDV) belongs to Nidoorder, family Coronaviridae, and genus Coronavirus, and is one of the main pathogens of swine viral diarrhea outbreaks. So far, porcine epidemic diarrhea (PED) caused by PEDV continues to be prevalent in the world. Porcine epidemic diarrhea is an acute intestinal infectious disease caused by virus in piglets and fattening pigs. Occurred in pigs and pigs of all ages can be infected and the mortality rate of newborn piglets is the highest, and sick pigs are the main source of infection. [0003] Porcine parvovirus (PPV) belongs to the family Parvoviridae and the genus Parvovirus. It is a reproductive disorder disease that causes pigs. The disease is mainly manifested as embryo and fetus in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K35/76A61K39/23A61P31/20A61K39/215A61P31/14C12R1/93
CPCC12N7/00A61K35/76A61K39/12A61P31/20A61P31/14C12N2750/14321C12N2750/14334A61K2039/70A61K2039/552C12N2770/20034A61K2039/5252
Inventor 张华伟徐高原余蕾周明光罗修鑫汤细彪倪冬冬
Owner WUHAN KEQIAN BIOLOGY CO LTD
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