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Cell growth regulator

A technology of cell growth regulator and regulator, which is applied in the field of biological cells, can solve the problems of short cell maintenance time, and achieve the effect of increasing virus content and poison price

Inactive Publication Date: 2014-03-26
YUNNAN ANIMAL SCI & VETERINARY INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a cell growth regulator, so as to solve the problem of short cell maintenance time

Method used

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Embodiment

[0022] A cell growth regulator, including the following components and quantities: ethyl ciprofloxacin: 100 mg; various cell extracts: 50-100ml; hydrolyzed milk protein: 5 grams; N-(2- Hydroxyethyl) piperazine-N'-2 sulfonic acid (HEPERS): 3 grams; minimum nutrient requirement medium (MEM): 1 liter; the prepared liquid was sterilized by filtration with a 0.2 μm filter membrane, and aliquoted at 4°C It can be stored for a short time, and it can be stored for a long time at ~20°C.

[0023] Add 40% to 60% of the cell growth maintenance regulator to the normal cell culture medium for cell culture according to the needs, and do not change the culture medium in the middle, so that the entire maintenance period of the cells can be extended by 2 to 3 times, which can be used for cell microneutralization experiments, viruses Plaque titration experiments, etc.

[0024] The grown cells are replaced with a maintenance solution containing 40% to 60% growth maintenance regulators before car...

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Abstract

The invention relates to a cell growth regulator. The cell growth regulator comprises the following ingredients by amount: 100 mg of ethyl ciprofloxacin, 50-100 ml of multi-cell extracting solution, 5 g of lactoalbumin hydrolysate, 3 g of N-(2-hydroxyethyl)piperazine-N'-2-sulfonic acid (HEPERS for short), and 1 L of culture medium with the lowest nutritional requirement. The preparation method comprises the steps of filtering the prepared liquid with a filter membrane of 0.2 microns to sterilize, split charging the filtered liquid for short term preservation at 4 DEG C, and long term preservation at -20 DEG C. According to the requirement, 40-60% of the cell growth maintaining regulator can be added in a normal cell culture fluid to culture cells; the culture fluid is not required to be changed in the halfway; the whole maintaining period of the cells is prolonged for 2-3 times; the cell growth regulator can be applied to a cell microneutralization test, a virus plaque titration test and the like. A maintenance medium containing 40-60% of the growth maintaining regulator is replaced for the grown cells before the biological test, and thus the cell maintaining period can be prolonged by about 2 times. The cell growth regulator can be applied to viral antigen production and the like.

Description

technical field [0001] The invention relates to a cell growth regulator and belongs to the technical field of biological cells. Background technique [0002] Tissue cell culture is a key technology in biology, which is widely used in various aspects such as biological research, virus vaccine antigen production, reagent production (such as monoclonal antibody) production and so on. Passage cells are a kind of cells that can be cultured for a long time. They are generally cultured in culture flasks. When the cells grow to a certain density, except for a small part that is passed down to expand the number of cells, most of them are used for various biological production and biological experiments. . Cells grown in culture flasks and used for biological experiments will age and die within 5 to 15 days at 37°C due to reasons such as too high cell density and depletion of nutrient solution. This period is called cell maintenance time, also known as cell maintenance time. It is t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 李乐高林苗海生何于雯李华春
Owner YUNNAN ANIMAL SCI & VETERINARY INST
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