62 results about "Roller Bottle" patented technology

Methods for the maximizing parameter of the in vitro growth and expansion of mammalian cells, specifically postpartum-derived cells in containers such as roller bottles is described. Methods of optimizing growth rate and cell yield in such culture systems are provided. The methods are particularly adapted for human postpartum-derived cells, such as umbilicus-derived cells.

The invention provides an advanced roller bottle system for cell culture that efficiently, continually, and automatically replenishes spent media with fresh media. The roller bottle system optimizes media use by removing spent media in response to a predetermined condition change and replenishing the spent media with fresh.

PCT No. PCT / GB95 / 01833 Sec. 371 Date Feb. 12, 1997 Sec. 102(e) Date Feb. 12, 1997 PCT Filed Aug. 2, 1995 PCT Pub. No. WO96 / 05285 PCT Pub. Date Feb. 22, 1996A cell culture apparatus is disclosed. The cell culture apparatus includes a chassis or supporting frame. The cell culture apparatus also includes a rotor releasably housing a plurality of cell culture vessels / roller bottles, the rotor being mounted about a substantially horizontal axis or shaft supported by said frame, with means provided to facilitate rotation of the rotor at a controlled speed. The cell culture apparatus also includes a reversible multi-channel pump or pumps, mounted on and rotating with the rotor. The cell culture apparatus also includes a manifold with one or more sealable external connections and a plurality of connections communicating with the individual channels of the multi-channel pump or pumps, each of the cell culture vessels being equipped with a microporous air vent to atmosphere and a dip tube. The dip tube is fixed with respect to the cell culture vessel and positioned to permit extraction of fluid when the cell culture vessel is stopped in a specific orientation. The dip tube of each vessel is individually connected to one channel of said multi-channel pump or pumps. The arrangement of the parts is such that the assembly comprising the rotor, cell culture vessels, pump, manifold and the connections to the cell culture vessels are rotatable about the horizontal axis. The arrangement also allows fluid to be injected into or extracted from the roller bottles simultaneously via a single external connection, under the influence of the multi-channel pump or pumps.

The invention provides an advanced roller bottle system for cell culture that efficiently, continually, and automatically replenishes spent media with fresh media. The roller bottle system optimizes media use by removing spent media in response to a predetermined condition change and replenishing the spent media with fresh.

The invention provides a roller bottle for cell growth culturing including a plurality of axial pleats therearound for increasing cell growth surface area and further including circumferential ribs integrally formed therewith for reinforcing the pleated wall structure. These circumferential ribs prevent the bottle walls from bowing outward when the inside of the bottle becomes pressurized during cell growth culturing.

A roller bottle is provided with a bottom wall, a top wall and a cylindrical side wall extending between the bottom and top walls. The top wall includes an opening to provide access to the interior of the roller bottle. The cylindrical side wall of the roller bottle is formed with at least one spiral pleat extending substantially from the bottom to the top for increasing cell growth surface area and for facilitating the flow of liquid to all interior surface areas of the bottle as the bottle is rolled about its longitudinal axis.

The invention discloses a culture medium applicable to suspension and magnification cultivation of Vero cell microcarriers. The culture medium comprises amino acid, inorganic salt, vitamins, protein hydrolysate, lipid, buffer components and additives. The culture medium not only is applicable to static cultivation in a Vero square bottle and cultivation in a roller bottle, but also is supportive for suspension cultivation of the microcarriers and magnification cultivation of the microcarriers in a reactor, and can be used as a serum-free virus maintenance medium to realize serum-free virus production.

The invention relates to a method for industrially producing a pseudorabies vaccine by using a bioreactor. The method comprises the following steps of: (1) sterilizing a microcarrier and the bioreactor, inoculating cells for vaccine production, culturing the cells, and after the cells on the microcarrier form a compact monolayer, inoculating pseudorabies virus, and continuously culturing to allow the virus to reproduce; (2) after more than 80 percent of cells are subjected to a cytopathic effect, stopping culturing, and obtaining virus liquid to prepare the pseudorabies vaccine. In the method, the pseudorabies vaccine is produced by culturing the cells in high density by the bioreactor microcarrier culture technology; and compared with the conventional roller bottle production process, the invention has the advantages that: the method has high automation control degree, saves manpower, reduces cost and is small in production land, the production can be monitored in real time, the scale is easy to expand, the produced virus has high titer and small difference among batches, and the product has stable quality and small side effect.

The invention discloses a serum-free medium suitable for large-scale single-cell suspension culture of young hamster kidney cells (BHK cells), including 21 kinds of amino acids, 11 kinds of inorganic salts, 12 kinds of vitamins, 1 kind of protein hydrolyzate, 2 kinds of Lipids, 2 buffer components and 6 additives. The BHK cell serum-free medium of the present invention produces biological products by single-cell suspension culture, which can not only avoid various disadvantages of serum culture, but also eliminate the problem of difficult scale-up of roller bottle adherent culture, thereby improving the production efficiency of BHK cell culture And reduce production costs, while ensuring product quality, has good application value and huge market prospects.

The invention discloses application of a baby hamster kidney(BHK)-21 cell serum-free suspension culture technology in foot-and-mouth disease vaccine production, which comprises the following steps of: 1) performing cell recovery; 2) performing reactor culture and cell amplification culture; and 3) inoculating foot-and-mouth disease virus seed venom and collecting the venom. A process for producing foot-and-mouth disease inactivated vaccines by culturing the BHK-21 cells through serum-free suspension culture and a step-by-step cell amplification method make the production period f the foot-and-mouth disease vaccines shortened and yield increased, and ensure stable quality and obvious benefit. The production process reduces the using amount of a culture medium, and the amount of the collected virus liquid is the culture medium consumption amount, while the culture medium consumption amount in a roller bottle production process is 2 times higher than the amount of the collected virus liquid, and bovine serum which is about 5 percent of the culture medium amount is needed.

The invention provides a culture method for a novel swine fever living vaccine cell roller bottle, which comprises the steps as follows: the spleen of a rabbit having stereotyped thermal reaction is collected as a virus seed by rabbit body immunity HCV; an ST monolayer cell is prepared, added with cell culture liquid and arranged into a roller bottle incubator of 37 DEG C for culture; the collected rabbit spleen having stereotyped thermal reaction is inoculated on the ST monolayer cell at a concentration of 0.2 to 0.3 percent; then the cell culture liquid is added and cultured in the roller bottle incubator of 37 DEG C; virus liquid 1 is collected after the mixture is cultured for 5 days; and then the virus liquid is collected every 4 days; the collected virus liquid is frozen and dried in vacuum, thus becoming the swine fever living vaccine. The invention aims at overcoming the defect of lower culture titre of the original cell line to HCV and can be used for improving the antigenicity of the swine fever living vaccine and enhancing the immunity of an organism.

A two-piece cap assembly for closing an opening in a neck portion of the container is provided that includes a cap body having a top wall and a depending annular skirt for screw attachment to the neck portion of the container. A plug seal is attachable to the underside of the top wall of the cap body. The plug seal and the cap body are relatively rotatably coupled for independent rotation of the cap body with respect to the plug seal upon attachment and detachment of the cap to the neck portion of the container.

The invention provides a domestication method of a full suspension culture type Marc-145 cell line. The domestication method comprises the following steps: (1) cultivating adherent-culture Marc-145 cells; (2) domesticating a low-serum adherent-culture Marc-145 cell line; (3) domesticating low-serum full suspension culture type Marc-145 cells; and (4) domesticating serum-free full suspension culture type Marc-145 cells. With the application of the cell domestication method disclosed by the invention, the Marc-145 cells of suspension culture can be obtained; the cells are adaptive to low-serum and serum-free full suspension culture; the density of the cells, when subjected to serum-free culture in a 7.5L reactor, can reach 8*10<6> / ml, and at least 4.4*10<10> cells can be obtained, equal to the number of cells obtained from 80-90 15L roller bottles or 18-20 40-layer cell plants; and the domestication method disclosed by the invention is significant in effect.

The instant invention employs an array of rods packed into a suitable chamber to support the culture, e.g., maintenance and / or growth of anchorage-dependent cells that effectively increases the surface area available for cell culture. Adherent cells are seeded and propagated on the surface of the rods. Each rod is accommodated with spacer devices that serve to make it immobile and ensure uniform access to liquid growth medium while minimizing abrasive damage to shear-sensitive cells. Spacer devices of various designs allow the rods to be packed and anchored into bioreactors, e.g., perfusion chambers with flow-through ports or into closed roller bottle-type chambers. Simple monomeric designs of the rods and spacer devices allow for relative ease of manufacture and assembly including solid or hollow rods or supports constructed of fibers or strings consisting of flexible tissue culture treated material that is held fast by threading or knotting between suitable spacer devices with eyelets, holes or other anchoring fixtures. In some embodiments, the rods are composed of tissue culture-treated plastic packed into culture chambers as prepackaged, sterile, disposable single use items.

The invention discloses a suspension culture production method for porcine circovirus 2-type cells; the method provided by the invention comprises the production technology of preparing porcine circovirus 2-type inactivated vaccines by suspension culture for poisoned cells through porcine circovirus 2-type virus biological reactor micro carriers. The method provided by the invention can greatly reduce production cost and increase output-input ratio by 5-10 times; and the method shortens production period by 2-3 days and occupies small places, wherein occupied area is only 1 / 5 of the occupied area in the traditional roller bottle process under the same yield; scale of production is enlarged fast and easily; pollution is little and processes are performed easily under the totally-enclosed tank production condition; degree of automation is high; employee is less; quality stability is realized easily; and the method can significantly reduce production cost and increase yield and quality of vaccines.

The invention discloses a method for amplifying mesenchymal stem cells of a human umbilical cord and a human placenta in vitro. The method comprises the following steps of: using human umbilical cord and placenta as a cell source; digesting tissues by using II type collagenase, centrifuging the product the digestion; removing supernate in the product of the centrifugation; washing the remaining product of the centrifugation by using D-Hank's liquid for three times to obtain single cell suspension; inoculating the single cell suspension in a culture plate or a culture flask; fully replacing the liquid when cell (P0) are adhered after 24 to 72 hours; when 80 to 90 percent of cells are mixed, performing subculture once (P1); inoculating the cells in a roller bottle according to a minimum density of 1,400 cells per cubic centimeter; comparing the growing status, cell amplification time and colony-forming capability of the mesenchymal stem cells between different culture systems to obtain an efficient and stable amplification culture system; and amplifying the mesenchymal stem cells in a large scale in the roller bottle by using the optimal amplification system.

A method of forming a hollow container, such as a roller bottle, having a bottom surface including an inwardly directed punt is provided, which uses air assist to eject the formed container. The method includes providing a blow mold having a pair of blow mold halves and a central blow mold portion defining a mold cavity. The central mold portion has an extended portion for defining the punt. The method further includes inserting a preform within the mold cavity and expanding the preform to form the container. Once the container is formed, the mold halves are opened and the central blow mold portion is withdrawn from the cavity while injecting gas into the mold cavity through a poppet valve in the central blow mold portion so as to release the formed container from the blow mold. In particular, blowing air between the plastic of the formed punt and the central blow mold portion prevents the central blow mold portion from sticking to the walls of the punt.

The invention provides a bottle state transition device for a full-automatic bottled oral liquid packing machine. The full-automatic bottled oral liquid packing machine comprises a vertical bottle feeding mechanism and a horizontal bottle discharging mechanism, the bottle state transition device comprises a bottle vertical-to-horizontal roller driving mechanism, a bottle vertical-to-horizontal roller, a vertical bottle channel guiding block and a horizontal bottle guiding-out block, the bottle vertical-to-horizontal roller driving mechanism is arranged on the left side of the rear end of the vertical bottle feeding mechanism and is connected with the vertical bottle feeding mechanism, the left end of the bottle vertical-to-horizontal roller is connected with the bottle vertical-to-horizontal roller driving mechanism, the right end of the bottle vertical-to-horizontal roller extends to the left end of the horizontal bottle discharging mechanism and supports on the horizontal bottle discharging mechanism, the vertical bottle channel guiding block is connected to the right side of the rear end of the vertical bottle feeding mechanism, and the horizontal bottle guiding-out block is fixed on the horizontal bottle discharging mechanism and further corresponds to the front of the middle part of the bottle vertical-to-horizontal roller simultaneously. Labor resources are saved, and sanitation is ensured; and pollution and resource waste are avoided.

The invention discloses an automatic ampoule scratching machine, which comprises a controller; a disinfection device and a scratching device which are sequentially arranged along the moving direction of a conveying part; the scratching device comprises a scratching assembly and a friction assembly; Provide friction to make the ampoule rotate along its own axis, and the scratching component scratches the neck of the ampoule; the disinfection device includes a primary disinfection box, a first roller and a second roller; the disposable disinfection box includes a A central cylinder outside the roller, on which the disinfectant medium for impregnating the disinfectant solution is wound. The device contacts the ampoule bottle through the friction component and exerts force to make it rotate along its own axis. The scratch component scratches the neck of the bottle. When the When the ampoule is sterilized, the roller drives the body of the ampoule to rotate, and the disinfection medium sterilizes the neck of the ampoule. During the rotation of the ampoule, the glass debris left on the circumference of the bottle due to the scratch operation is taken away to avoid opening the ampoule. , glass shards fell into the bottle.

The invention provides a method of utilizing a stirred bioreactor to produce an infectious Bursal disease virus. Bioreactor microcarrier cell culture technology is used to replace conventional roller bottle culture, so that the problems of low production efficiency, unstable product quality and low virus titer can be solved. On this basis, biological characteristics of the infectious Bursal disease virus and DF1 cells are combined, and proper conditions are matched from the perspectives of microcarrier adding amount, cell inoculation density, virus inoculation amount, cell density during virus inoculation, virus collection time and reactor operation parameters, so that virus culture efficiency is improved remarkably, and unit culture titer is improved by 10-100 times. In addition, compared with roller bottle culture, the method utilizing the bioreactor has the advantages that culture scale is large, and parameter control is comprehensive, so that systematic risk of being polluted is lowered, quality stability is improved, and the method has a wide application prospect.

The invention relates to animal vaccines, and specifically discloses an antigen of an epidemic encephalitis live vaccine and a preparation method and application thereof. Vero cells are adopted to carry out roller bottle culture on epidemic encephalitis virus attenuated strains (SA14-14-2 strains); virus culture maintenance solution which comprises components such as 4-hydroxyethylpiperazine ethane sulfonic acid, arginine monohydrochloride and PEG2000 is used; the produced antigen has the advantages of being small in batch difference, good in immunogenicity and high in virus titer; and the epidemic encephalitis live vaccine prepared through the antigen is capable of effectively preventing the epidemic encephalitis virus infection of pigs.

The invention discloses a bottle standing-rotating-lying posture converting device of a bottled oral liquid packaging machine. The bottled oral liquid packaging machine comprises a bottle input mechanism and a bottle output mechanism, the bottle input mechanism comprises a bottle input conveying belt and a pair of bottle guiding rails, the two ends of the bottle input conveying belt are arranged on a bottle input conveying belt roller in a sleeving mode, the bottle input conveying belt roller is arranged on a bottle input rack, the bottle input rack is supported to the grade level, and the pair of bottle guiding rails is fixed to the bottle input rack; the left end of the bottle output mechanism is connected with the front side of the right end of the bottle input rack, the bottle standing-rotating-lying posture converting device comprises a rotary disc platform, the left side of the rotary disc platform is fixed to the bottle input rack, the front side of the rotary disc platform is fixed to the upper portion of the rear side of the left end of the bottle output mechanism, and a bottle blocking mechanism is arranged on the rotary disc platform; and a bottle conveying rotary disc is connected with a bottle conveying rotary disc driving mechanism, and the bottle conveying rotary disc driving mechanism is supported to the grade level. Labor resources are saved, sanitation is guaranteed, and the situation of bottle fragmentation in the standing, rotating and lying processes is avoided.

The invention discloses application of static constant magnetic field used for prolonging hold time of primary cell, including that cells are placed in a magnetic field to be cultured and the magnetic field is arranged on the outer wall close to the cells. Concretely, the magnetic field is arranged at the bottom of a cell culture plate when the cell is cultured by adopting the cell culture plate; the magnetic filed is arranged at the bottom of a cell culture bottle when the cell is cultured by adopting the cell culture bottle; and the magnetic field is arranged on the outer wall of a roller bottle when the cell is cultured by adopting the roller bottle. The magnetic field is a static constant one, and magnetic force is 500-3000 Gauss. The primary cell can be histocytes coming from all animals including human, such as hepatocyte, nephrocyte, lens cell and testicular cell. The invention adopts a manner of adding a magnetic field into the cell culture environment to prolong hold time of primary cell in vitro, the hold time of cell can be more than 30 days, and cell growth is vigorous, thus effect is good.

The invention provides a preparation method of a PCV2, PRRSV and mycoplasma hyopneumoniae triple inactivated vaccine. The technical scheme is that a brand-new preparation method is designed from culture, inactivation, complex and other levels based on growth characteristics of three microorganisms and physicochemical characteristics of an antigen substance. In the method, the PCV2 and the PRRSV are prepared by adopting a bioreactor mode; in the preparation process of an antigen, a pH value of fermented liquid is strictly controlled, so that the immunogenicity of a virus is effectively ensured;mycoplasam suis produces a strain in a bacteria fermenting tank; and in the breeding process of the strain, the homogenization of an intra-batch bacterial solution is ensured by setting dissolved oxygen, the pH value, rotating speed and the like, the defects of complex operation, high working intensity, easiness in pollution and the like in the rolling bottle culture process are eliminated, and the quality stability of the mycoplasam suis inactivated vaccine is effectively improved. Experiments show that the protection rates of the prepared triple inactivated vaccine for the PCV2, the PRRSV and the mycoplasma hyopneumoniae all exceed 80 percent, and preventing and treating demands on three pathogens are met.

The invention provides a device and method for replacing a charged roller bottle in a 10 kV power distribution network, wherein the device and method are convenient to operate and can improve charged work efficiency. The device comprises a multi-layer umbrella-shaped insulator. A wire hook is arranged at the upper end of the umbrella-shaped insulator and movably connected with the umbrella-shaped insulator. A rubber resistance and protection layer is arranged on the surface of the wire hook. A lifting screw is fixedly connected with the lower end of the umbrella-shaped insulator. An operation spanner is arranged at the bottom of the lifting screw. A U-shaped fixing support is connected to the lifting screw through threads. A fastening screw is arranged at the bottom of the fixing support. During operation, the U-shaped fixing support is fastened to a right-angle cross arm, and the wire hook is rotated to the position below a wire of the roller bottle to be replaced; a screw connected with the wire is detached, the lifting screw is rotated, the wire is lifted up, and a screw fixedly connected with the cross arm is detached; a new roller bottle is fixedly connected with the cross arm, the lifting screw is rotated backward, the fastening screw is released, and the device for replacing the charged roller bottle is detached.

The invention discloses a large-area culture roller bottle for biological cell culture. A plurality of annular culture cavities are formed through the cooperation of a plurality of inner ring bottles, so that the actual culture area is increased in a disguised mode; the multiple inner ring bottles are separated, so that the culture of different cells can be conducted at the same time; and nutrient solutions in the annular culture cavities are prevented from overflowing by utilizing an inner bent part and an outer bent part. The large-area culture roller bottle is characterized in that one end of a top sealing cover covers one end of an outer culture bottle; an air hole is formed in the middle of one end of the top sealing cover; an air-permeable film is arranged on the air hole; the air-permeable film is made of transparent materials; the other end of the outer culture bottle extends in the axial direction of the outer culture bottle, then is bent towards the outer portion of the outer culture bottle and extends in the axial direction of the outer culture bottle; the outer culture bottle is bent to form the outer bent part; and the bending angle of the outer bent part ranges from 45 degrees to 75 degrees.