Application of baby hamster kidney(BHK)-21 cell serum-free suspension culture technology in foot-and-mouth disease vaccine production
A BHK-21, 1. BHK-21 technology, applied in the field of foot-and-mouth disease vaccine production, can solve the problems of large consumption of raw materials, long complex cycle, large side reactions, etc., to achieve product quality improvement, quality stability, cycle shortening Effect
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[0030] 1. Source of materials:
[0031] 1. Materials:
[0032] 1) Cell line: BHK-21 cells cultured in suspension (provided by Gansu Animal Cell Engineering Technology Research Center);
[0033] 2) Serum-free medium (dry powder), purchased from Lanzhou Bailing Biotechnology Co., Ltd., batch number: 20100420;
[0034] 3) FMD virus strain, OS 99 strain and Aasi-I strain were provided by Xinjiang Tiankang Animal Husbandry Biotechnology Co., Ltd.
[0035] 2. Instruments and equipment:
[0036] 1) Bioreactor:
[0037] a) B.Braun, model: BIOSTATEOR B plus, culture tank volume 5L;
[0038] b) Beijing Qingda Tianyi Technology Co., Ltd., model: CLAVORUS TM PLJ120, the volume of the culture tank is 120L;
[0039] c) Beijing Qingda Tianyi Technology Co., Ltd., model: CLAVORUS TM PLJ650, the volume of the culture tank is 650L;
[0040] 2) Shaker, Shanghai Kanxin Instrument Equipment Co., Ltd., model: TZ-05;
[0041] 3) Magnetic stirrer, Shanghai Meiyingpu Instrument Manufacturing C...
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