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Production process for improving titer of swine fever live vaccine product

A swine fever live vaccine and production technology technology, which is applied in the field of production technology for improving the titer of swine fever live vaccine products, can solve the problems of low stock quantity, increase the number of heads, and difficulty in production, so as to improve the titer and quality, prolong the The effect of shelf life and drug price increase

Active Publication Date: 2015-05-06
兆丰华生物科技(福州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This production process mainly has the following two problems: 1) the storage time of semi-finished products is short, and at -15°C, the storage period is no more than 15 days and the loss of virus titer is large, especially in summer. High heat stress during transportation affects product quality and makes production difficult; 2) The current product potency can only reach 150 rabbit body infections
Clinical use tends to increase the dose, resulting in large side effects

Method used

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  • Production process for improving titer of swine fever live vaccine product
  • Production process for improving titer of swine fever live vaccine product
  • Production process for improving titer of swine fever live vaccine product

Examples

Experimental program
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Effect test

preparation example Construction

[0031] 3) Preparation of swine fever live vaccine:

[0032] a. Inoculation: Make a 20-50 times diluted emulsion with a special protective agent for swine fever virus for the production of virus seeds, and inject 1 mL into each rabbit's ear vein;

[0033] b. Temperature measurement: the operation is the same as b in step 2);

[0034] c. Harvest of semi-finished products: select rabbits with stereotyped heat or light heat reaction, dissect them within 24 hours after the body temperature drops, and collect spleen and lymph with aseptic operation to obtain semi-finished products;

[0035] d. Preservation of semi-finished products: After adding 200-300mL of special protective agent for spleen-leaching tissue per kilogram to the semi-finished products, store them in a freezer below -15°C; the storage time should not exceed 60 days;

[0036] 4) Mixing seedlings, packaging and freeze-drying: under aseptic operation, weigh and cut the semi-finished products into pieces, add 5% sucrose...

Embodiment 1

[0042] A kind of production technology that improves the product potency of swine fever live vaccine, it comprises the following steps:

[0043] 1) Selection of rabbits: select healthy rabbits with a weight of 1.5-3.0kg, measure and observe the temperature for more than 3 days before inoculation, and the body temperature needs to be normal;

[0044] 2) Propagation of poisonous species:

[0045] a. Inoculation: make a 20-fold diluted emulsion with a special protective agent for CSF virus, and inject 0.5 mL into each rabbit's ear vein;

[0046] b. Temperature measurement: 24 hours after the rabbit was inoculated, the body temperature was measured every 6 hours;

[0047] c. Harvesting of production virus seeds: select the rabbits with stereotyped heat response, dissect them within 24 hours after the body temperature drops, take the spleen by aseptic operation, and freeze the spleen at -15°C as the production virus seeds;

[0048] d. Identification of production poisonous specie...

Embodiment 2

[0069] A kind of production technology that improves the product potency of swine fever live vaccine, it comprises the following steps:

[0070] 1) Selection of rabbits: select healthy rabbits with a weight of 1.5-3.0kg, measure and observe the temperature for more than 3 days before inoculation, and the body temperature needs to be normal;

[0071] 2) Propagation of poisonous species:

[0072] a. Inoculation: make a 50-fold diluted emulsion with a special protective agent for CSF virus, and inject 2 mL into each rabbit's ear vein;

[0073] b. Temperature measurement: 24 hours after the rabbit was inoculated, the body temperature was measured every 8 hours;

[0074] c. Harvesting of production virus seeds: select the rabbits with stereotyped heat response, dissect them within 24 hours after the body temperature drops, take the spleen by aseptic operation, and freeze the spleen at -15°C as the production virus seeds;

[0075] d. Identification of production poisonous species:...

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Abstract

The invention relates to a production process for improving titer of a swine fever live vaccine product, which comprises the following steps of selecting rabbit, breeding a production virus seed, preparing a swine fever live vaccine, producing the vaccine, subpackaging, lyophilizing and inspecting a finished product. The virus seed is diluted by adopting a swine fever virus specific protective agent in vaccination, and can quickly colonize and propagate in a target cell when entering a rabbit body, so that the virus titer can be increased; and loss of the swine fever virus can be reduced by using a specific spleen and lymph tissue protective agent, so that the preservation period of a semi-finished product can be prolonged, and the titer and quality of the product can be improved, wherein the preservation period of the semi-finished product is prolonged to 60 days from the previous 15 days, and the titer of the finished product is increased from the previous infective dose of 150 rabbits to the infective dose of 900 rabbits.

Description

technical field [0001] The invention relates to the field of veterinary medicine and biology, in particular to a production process for improving the product titer of swine fever live vaccine. Background technique [0002] Swine fever is a highly contagious and fatal swine infectious disease caused by swine fever virus. It occurs in many countries and regions in the world. It is a devastating infectious disease in the pig industry. There have been serious economic losses. The World Organization for Animal Health lists swine fever as a legally notifiable disease, and my country classifies it as a first-class animal infectious disease. In the 1950s, China first created the world-famous attenuated CSF vaccine (strain C), which was screened out from the virulent strain of CSF Shimen strain through passage in rabbits for more than 400 generations. Strain C is a very safe attenuated vaccine. It has no side effects on pigs of various ages and breeds, and has good immune efficacy....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/187C12N7/00A61P31/14
Inventor 林志坤吴润生江兴华
Owner 兆丰华生物科技(福州)有限公司
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