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Chicken newcastle disease virus inactivated vaccine preparation method

A technology of chicken Newcastle disease virus and inactivated vaccines, applied in the field of veterinary vaccines, can solve the problems of difficult absorption, high resistance, toxic and side effects, etc., and achieve high protection rate, reduced quality difference, and good immunogenicity

Inactive Publication Date: 2017-05-31
鼎正生物药业(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the main method to control the incidence of chicken Newcastle disease virus is to inoculate inactivated whole virus vaccine, and to control typical chicken reovirus infection through immunization, but most of the inactivated vaccines are water-in-oil type at present, and the resistance during injection is large , not easy to absorb, and has certain toxic and side effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A preparation method of chicken Newcastle disease virus inactivated vaccine comprises the following steps:

[0018] 1) Prepare primary CEF cells, and inoculate chicken Newcastle disease virus when the cells grow to a monolayer.

[0019] 2) When more than 80% of the cells have lesions, the cell culture is harvested, frozen and thawed repeatedly 3 times, and the cell venom containing the supernatant is obtained.

[0020] 3) Determine the virus titer, the virus titer should be ≥ 10 6.5 TCID 50 / 0.1mL.

[0021] 4) Place the qualified cell venom in a sterile container for inactivation treatment, add formaldehyde solution and shake well, the amount of formaldehyde solution added is 2‰ of the volume of the inactivation solution, control the temperature at 37 ° C for 48 hours, and rotate It is 180r / min.

[0022] 5) The inactivated virus is subjected to an inactivation test to determine the optimum inactivation time and formaldehyde concentration.

[0023] 6) Mix the inacti...

Embodiment 2

[0025] A preparation method of chicken Newcastle disease virus inactivated vaccine comprises the following steps:

[0026] 1) Prepare primary CEF cells, and inoculate chicken Newcastle disease virus when the cells grow to a monolayer.

[0027] 2) When more than 80% of the cells have lesions, the cell culture is harvested, frozen and thawed repeatedly 3 times, and the cell venom containing the supernatant is obtained.

[0028] 3) Determine the virus titer, the virus titer should be ≥ 10 6.5 TCID 50 / 0.1mL.

[0029] 4) Place the qualified cell venom in a sterile container for inactivation treatment, add formaldehyde solution and shake well, the amount of formaldehyde solution added is 0.5‰ of the volume of the inactivation solution, control the temperature at 37 ° C for 12 hours, and rotate It is 180r / min.

[0030] 5) The inactivated virus is subjected to an inactivation test to determine the optimum inactivation time and formaldehyde concentration.

[0031] 6) Mix the inac...

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Abstract

The invention provides a chicken newcastle disease virus inactivated vaccine preparation method. According to the present invention, inoculation with chick embryo fibroblasts is used to replace inoculation with chick embryo allantocherion to breed chicken newcastle disease viruses, such that the vaccine preparation cost is saved, the quality difference between the batches is reduced, the yield is high, and the virus titer and the vaccine quality are improved; the vaccine is emulsified with the homogenizer, such that the prepared vaccine has advantages of low viscosity, small particle size and easy absorption compared to the traditional water-in-oil dosage form; and any surfactant components are not required to be added during the vaccine preparation process through the selected adjuvant, the prepared vaccine has good safety, and the side effect due to the difficult absorption of the traditional oil adjuvant is avoided.

Description

technical field [0001] The invention relates to the technical field of veterinary vaccines, in particular to a method for preparing chicken Newcastle disease virus inactivated vaccines. Background technique [0002] Chicken Newcastle disease virus belongs to Paramyxoviridae (Paramyxoviridae) genus Paramyxovirus (Paramyxovirus) on classification. Mature virions are nearly round, mostly tadpole-shaped, with a diameter of 120-300nm. It has a capsule, which contains a long helical nucleocapsid with a diameter of 17-18nm, and 8-12nm long glycoprotein (HN and F) fibers outside the capsule. The helical nucleocapsid is formed by a single-stranded RNA associated with a protein and has RNA polymerase activity. It is wrapped by a double-layer lipid membrane, and the lipid membrane is lined with a special layer of M protein, while the lipid The outer layer of the plasma membrane is covered by glycoproteins with filaments, giving it a flower spike shape. [0003] In the prior art, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/17A61P31/14
Inventor 刘鼎阔王立红张凤洪张俊霞
Owner 鼎正生物药业(天津)有限公司
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