Production method of hog cholera live vaccine

Abstract

The invention relates to a production method of hog cholera live vaccine, comprising the following technical steps: (1) cloning and identifying a hog cholera lapinized low virulent strain; (2) breeding cloned strain seed virus; (3) breeding cells for production; (4) breeding virus liquid for production; and (5) matching vaccine, carrying out split charging and freeze-drying. The method provided by the invention has the characteristics of simple and stable production technology, good virus immunogenicity and genetic stability, small risk of exogenous virus pollution, low production cost and the like, and is convenient for inspection. The hog cholera live vaccine produced by the production method has the advantages of good genetic stability, high purity and strong immunogenicity.

Description

technical field [0001] The invention relates to a production method of a live swine fever vaccine and belongs to the technical field of veterinary biological products. Background technique [0002] Classical Swine Fever (CSF) is a highly contagious and fatal swine infectious disease caused by Classical Swine Fever Virus (CSFV), which occurs in many countries and regions in the world. Industrial production has brought serious economic losses. The World Organization for Animal Health (OIE) lists swine fever as a statutory notifiable disease, and my country classifies it as a first-class animal infectious disease. [0003] At present, there are mainly 3 kinds of live swine fever vaccines with good effects in the world, namely the GPE strain in Japan, the Thiverval strain in France and the attenuated rabbit strain (C strain) in China, among which the attenuated rabbit strain is the most widely used and is the world's leading It has made important contributions to the control a...

AI Technical Summary

Problems solved by technology

Among them, GPE strain and Thiverval strain are produced by pig-derived cell lines, which have a greater risk of known or unknown exogenous virus contamination. Once they are not well controlled, they can easily lead to certain existing or newly discovered infectious diseases popular

Among them, there are currently two production processes for rabbitized attenuated live vaccines. The first process is to use rabbits to produce vaccines. The tissue or lymph nodes or spleens of rabbits inoculated are used to make vaccines. This process can effectively avoid foreign virus contamination and ensure the virus The genetic stability of the virus does not become stronger, but it needs to use a large number of rabbits, the quality control is difficult and the production cost is high; the second process is the production of cattle, sheep primary cells or pig passage cells. Avoid using a large number of experimental animals, but there is a risk of exogenous virus contamination (because bovine, sheep primary cells or pig passage cells are susceptible to many known or unknown exogenous viruses) and the genetic stability of the virus is slightly poor, and the virus has an independent The risk of returning to strength

In addition, the attenuated swine fever rabbit virus is domesticated through continuous passage of rabbits, and the seed virus has not been cloned and purified. Therefore, different batches of seed poisons are different from the same batch of rabbits or the same batch of seed poisons from different batches. The infectivity and immunogenicity of rabbits often have certain differences