Cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and preparation method of cell affinity agent
A technology for in vitro infection and duck yellow virus, applied in the direction of virus/bacteriophage, microbe-based methods, biochemical equipment and methods, etc., can solve the problems of low cell infection efficiency, insufficient virus titer, slow primary virus culture process, etc. problems, to achieve the effect of improving in vitro infection efficiency and increasing affinity
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Embodiment 1
[0039] A kind of cell substantivity agent that improves duck yellow virus in vitro infection efficiency, it is prepared from the following components by mass percentage:
[0040]
[0041] Wherein, the inorganic salt is a mixture of sodium sulfate, disodium hydrogen phosphate, and potassium chloride.
[0042] Wherein, the sodium sulfate accounts for 0.3% of the total affinity agent; the sodium dihydrogen phosphate accounts for 1.6% of the total affinity agent; and the potassium chloride accounts for 1% of the total affinity agent.
[0043] Specifically, the affinity agent is prepared according to the following steps: weigh malonic acid, phosphoric acid, sorbic acid, malic acid, sodium sulfate, disodium hydrogen phosphate, potassium chloride according to the formula, Dissolve in deionized water according to formula and filter.
Embodiment 2
[0045] A kind of cell substantivity agent that improves duck yellow virus in vitro infection efficiency, it is prepared from the following components by mass percentage:
[0046]
[0047] Wherein, the inorganic salt is a mixture of sodium sulfate, disodium hydrogen phosphate, potassium chloride and sodium chloride.
[0048] Wherein, the sodium sulfate accounts for 0.25% of the total affinity agent; the sodium dihydrogen phosphate accounts for 1% of the total affinity agent; the potassium chloride accounts for 0.5% of the total affinity agent; The sodium chloride accounts for 0.75% of the total affinity agent.
[0049] Specifically, the affinity agent is prepared according to the following steps: weigh malonic acid, phosphoric acid, sorbic acid, malic acid, sodium sulfate, disodium hydrogen phosphate, potassium chloride, sodium chloride , Dissolve the above components in the deionized water of the formula, and filter to obtain.
Embodiment 3
[0051] A kind of cell substantivity agent that improves duck yellow virus in vitro infection efficiency, it is prepared from the following components by mass percentage:
[0052]
[0053]
[0054] Wherein, the inorganic salt is a mixture of sodium sulfate, disodium hydrogen phosphate, and sodium chloride.
[0055] Wherein, the sodium sulfate accounts for 0.2% of the total affinity agent; the sodium dihydrogen phosphate accounts for 1.5% of the total affinity agent; and the sodium chloride accounts for 1% of the total affinity agent.
[0056] Specifically, the affinity agent is prepared according to the following steps: weigh malonic acid, phosphoric acid, sorbic acid, malic acid, sodium sulfate, disodium hydrogen phosphate, sodium chloride according to the formula, Dissolve in deionized water according to formula and filter.
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