30results about How to "Change the microenvironment" patented technology

The invention belongs to the field of biotechnology, and specifically relates to an immobilized horseradish peroxidase carrier and its preparation and application method, wherein the immobilized horseradish peroxidase carrier is nano-carbon spheres prepared by hydrothermal carbonization of glucose , and then it is obtained by amination modification and glutaraldehyde activation; the immobilized horseradish peroxidase carrier of the present invention is mixed and shaken with the horseradish peroxidase solution, centrifuged, washed and freeze-dried to obtain the immobilized horseradish peroxidase root peroxidase. The invention provides a green, economical and efficient method for preparing immobilized horseradish peroxidase, the immobilized horseradish peroxidase obtained by the method is low in cost, simple in operation, stable in physical and chemical properties, especially in extreme environments High catalytic activity can still be maintained under these conditions.

The invention discloses a high-throughput microfluidic chip and a cell analysis device and method. The high-throughput microfluidic chip comprises a cell observation pool, a stimulant channel, a cell introduction channel, a buffer solution channel and a waste liquid channel, wherein the stimulant channel is connected with the opening of the cell observation pool, the cell introduction channel is connected with the side face of the cell observation pool, the connecting site is close to the opening, the buffer solution channel is connected with the bottom of the cell observation pool, and the waste liquid channel is connected with the opening of the cell observation pool. The cell analysis device comprises the high-throughput microfluidic chip, a signal collection device and electric proportional switching valves, and the channel inlet end of the high-throughput microfluidic chip is connected with independent electric proportional switching valves; the signal collection device is arranged at the cell observation pool and is used for collecting cell signals or imaging cells. The analysis method comprises the following steps: capturing cells, stimulating cells and releasing cells. The chip, the device and the method provided by the invention can be used for achieving high-throughput cell stimulation and analysis in multiple modes.

The invention discloses a ptycene-perylene diimide derivative, a synthesis method thereof and sensing application to gas-phase volatile aromatic hydrocarbon. The ptycene-perylene diimide derivative has a special non-planar structure, so that a prepared sensing film has large specific surface area and porosity, and is rich in molecular channels, and good diffusion of molecules of an object to be detected in the film can be ensured; and meanwhile, by utilizing a capillary condensation phenomenon and the sensitive effect of fluorescent molecules on a surrounding environment in a high-excitation state, high sensitivity and high selectivity response to BTEX gas are realized, the response time and the recovery time are short, and a new thought is provided for gas-phase sensing of volatile organic compounds lacking photoelectric activity and preparation of a fluorescent sensing film with excellent performance. The preparation method is simple and convenient to operate and mild in reaction condition, the photochemistry stability and thermodynamic stability of a prepared fluorescent compound are good, and the obtained fluorescent sensing film is high in sensitivity, good in selectivity andlong in service life, and is an excellent fluorescent sensing film.

The invention discloses a cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and a preparation method of the cell affinity agent and belongs to the technical field of agents for microbial virus culture. The cell affinity agent is prepared from the following components in percentage by mass: 0.5-5% of organic acid, 0-1% of phosphoric acid, 0-3.5% of inorganic salt and the balance of deionized water. By the cell affinity agent, the microenvironment between the duck flavivirus and the cell can be improved, and the affinity between the duck flavivirus and the cell is increased so that the infection efficiency of the duck flavivirus to the cell is effectively increased.

The invention discloses a cultivation method for wedge plowing and transplanting rice, belonging to the field of rice cultivation. To solve the problem of the low yield of high-yield high-quality varieties in the exiting cultivation mode, the cultivation method of the invention comprises the following steps: (1) plowing soil; not carrying out plowing and watering after harvesting former crop in rotation; and digging a plurality of ditches on a surface of a farmland, wherein the row spacing between two adjacent ditches is 20cm, the ditch is narrow at the bottom and wide at the top and has an inverted triangular shape, the depth of the ditch is 5-10cm, the width of the ditch is 3-6cm, the two side faces of the ditch are all oblique planes relative to a horizontal plane, and the included angle alpha is 40-70 degrees between the oblique plane and the horizontal plane; (2) applying base fertilizer, transplanting seedlings, and watering; and (3) subsequently managing the field. The cultivation method of the invention reduces the farming intensity by using wedge plowing instead of traditional ploughing, applies fertilizer to the bottom of the wedge-type ditch in a centralized way, reduces the disturbance to the soil, is energy-saving and labor-saving, has large water storage capacity of the soil and high soil system stability, increases the utilization ratio of fertilizer, improves the growth environment of root system, and realizes the effects of saving cost and increasing yield and efficiency.

The invention relates to an external traditional Chinese medicine composition and preparation for treating nodular type acnes and a preparation method thereof. The composition comprises the following raw materials in parts by weight: 5-10 of nagkassar seeds, 10-25 of bark of magnolia, 10-20 of Chinese angelica, 20-30 of cajeput leaves, 10-20 of alfalfa, and 5-10 of motherwort. The traditional Chinese medicine extract provided by the invention has the advantages of eliminating causes of disease causing arcane inflammation by controlling bacteria breeding on the one hand and improving skin quality by controlling sebum secretion and conditioning the water-oil balance of skins on the other hand; and more importantly, the traditional Chinese medicine extract can improve skin microcirculation, change skin microenvironment and fundamentally eliminate nodular type acne cyst, acne marks and scars.

The invention provides a high-efficiency planting method of a little red, which relates to the technical field of cultivation of Chinese medicinal materials. The high-efficient planting method of a little red of the present invention comprises the following steps: (1) selecting seeds, (2) preparing a germination accelerator, (3) Preparation of ecological mulch, (4) ridging, (5) seed pretreatment, (6) sowing and (7) field management; the high-efficiency planting method of a little red of the present invention has a high germination rate and a fast germination speed, which avoids to the greatest extent Manual weeding greatly saves production costs, improves the economic benefits of a little red planting, is good for the environment, and has no pollution, so it is worth promoting.

The invention discloses a cultivation method for rice direct dibble seeding in wedged ditches, which belongs to the field of rice cultivation. In order to solve the problem that yield potential of high-yield high-quality varieties is difficult to fulfill by existing cultivation methods, the cultivation method for rice direct dibble seeding in wedged ditches includes the steps: firstly, tilling soil, namely performing no turning or irrigation after preceding harvesting, and digging a field to obtain a plurality of ditches, wherein row spacing between each two adjacent ditches is 18-22cm, each ditch in a triangular shape is wide end up, each ditch is 5-20cm deep and 3-6cm wide, two sides of each ditch are slopes relative to the horizontal plane, and an included angle between each slope and the horizontal plane is 40 DEG-70 DEG; secondly, post-treating rice seeds; thirdly, performing base fertilizer application, direct dibble seeding, and irrigation; and fourthly, performing field management. The traditional ploughing is replaced with wedging, so that farming intensity is reduced, interference to soil is lowered, energy sources are saved, labor is reduced, seeds for use are few, soilwater retention capacity is high, soil system stable is high, fertilizer utilization rate is high, root system growth environment is improved, and cost reducing, yield increase and efficiency enhancement are achieved.

The invention provides a gold(III) complex-perylene diimide derivative and a fluorescence sensing tube and tube-type fluorescence sensor prepared therefrom. The alkyne-based (III) structure is introduced into the perylene diimide anhydride site modified by the curved position, and the non-planarity of the formed structure effectively suppresses the strong intermolecular stacking effect of the perylene diimide and avoids aggregation-induced fluorescence quenching. The cyclometalated Au(III) complex and the perylene diimide can also form an ideal energy donor-acceptor pair, thereby effectively broadening the surface of the system. The Stokes displacement is observed, which lays the foundation for the structural optimization of the sensor to be built. The gold(III) complex-perylene diimide derivative has good fluorescence properties, and the tubular fluorescence sensor has high sensitivity.