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High-throughput microfluidic chip and cell analysis device and method

A microfluidic chip and analysis device technology, applied in the field of medical devices, can solve the problems of low throughput and complicated high-throughput cell collection process, and achieve the effect of simple structure and controllable stimulation time

Active Publication Date: 2015-04-08
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides a high-throughput microfluidic chip, a cell analysis device and a method, the purpose of which is to achieve cell repetition through the planar design and pressure operation of the microfluidic chip Capture and stimulation, thereby solving the technical problems of complex and low-throughput cell collection in existing microfluidic chips

Method used

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  • High-throughput microfluidic chip and cell analysis device and method
  • High-throughput microfluidic chip and cell analysis device and method
  • High-throughput microfluidic chip and cell analysis device and method

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Embodiment 1

[0043] A microfluidic chip is a polydimethylsiloxane (PDMS) microfluidic chip, which is formed by bonding a polydimethylsiloxane (PDMS) thin layer with a microchannel structure and a glass sheet , the chip height is preferably 30 μm.

[0044] The surface of the PDMS thin layer is processed with a microchannel structure, and its local microstructure is as follows: figure 1 As shown, it includes a cell observation pool 1, a stimulus channel 2, a cell introduction channel 3, a buffer channel 4 and a waste channel 5.

[0045] The cell observation pool 1 described in this embodiment is used to capture cells and observe the state of the cells. There is a figure-eight microstructure at the bottom, the wide mouth of the figure-eight microstructure faces the mouth of the cell observation pool 1, and its narrow mouth faces the cell observation pool 1 At the bottom, the specific size of the eight-shaped microstructure is as follows figure 2 shown. The stimulator channel 2 is a microc...

Embodiment 2

[0052] A microfluidic chip is a polydimethylsiloxane (PDMS) microfluidic chip, which is formed by bonding a polydimethylsiloxane (PDMS) thin layer with a microchannel structure and a glass sheet , the chip height is preferably 30 μm.

[0053] The surface of the PDMS thin layer is processed with a microchannel structure, and its local microstructure is as follows: Figure 5 As shown, it includes a cell observation pool 1, a stimulus channel 2, a cell introduction channel 3, a buffer channel 4 and a waste channel 5.

[0054] The cell observation pool 1 described in this embodiment is used to capture cells and observe the state of the cells. There is a figure-eight microstructure at the bottom, the wide mouth of the figure-eight microstructure faces the mouth of the cell observation pool 1, and its narrow mouth faces the cell observation pool 1 bottom. There are two stimulant channels 2, which are connected to the mouth of the cell observation pool 1, and are used to introduce ...

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Abstract

The invention discloses a high-throughput microfluidic chip and a cell analysis device and method. The high-throughput microfluidic chip comprises a cell observation pool, a stimulant channel, a cell introduction channel, a buffer solution channel and a waste liquid channel, wherein the stimulant channel is connected with the opening of the cell observation pool, the cell introduction channel is connected with the side face of the cell observation pool, the connecting site is close to the opening, the buffer solution channel is connected with the bottom of the cell observation pool, and the waste liquid channel is connected with the opening of the cell observation pool. The cell analysis device comprises the high-throughput microfluidic chip, a signal collection device and electric proportional switching valves, and the channel inlet end of the high-throughput microfluidic chip is connected with independent electric proportional switching valves; the signal collection device is arranged at the cell observation pool and is used for collecting cell signals or imaging cells. The analysis method comprises the following steps: capturing cells, stimulating cells and releasing cells. The chip, the device and the method provided by the invention can be used for achieving high-throughput cell stimulation and analysis in multiple modes.

Description

technical field [0001] The invention belongs to the field of medical devices, and more specifically relates to a high-throughput microfluidic chip, a cell analysis device and a method. Background technique [0002] Cells are the basic unit of all known life in nature except viruses, and the basis of all life activities. Therefore, in-depth research on cells is the key to uncovering the mysteries of life and transforming life. The ability of cells to recognize and respond to their microenvironment through signal transduction is crucial for the development, tissue repair, and immunity of organisms. When cells are stimulated by different chemical drugs, they will trigger the spatiotemporal changes of cell response, regulate the electrophysiological activity of cells, thereby affecting the growth and differentiation of cells and maintaining the physiological balance of cells. [0003] Many dynamic intracellular reactions, such as protein transport, protein interaction, post-tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/34C12Q1/04
CPCB01L3/502761B01L2200/0647B01L2400/0487C12M23/16C12M33/00C12M33/12G01N33/5008
Inventor 冯晓均陈鹏刘笔锋郭怡然李一伟
Owner HUAZHONG UNIV OF SCI & TECH
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