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Tube seedling slow growth conservation method for anoectochilus roxburghii germplasm

A preservation method and test-tube seedling technology, which are applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effects of lowering the cultivation temperature, reducing the light intensity, and reducing the number of generations.

Inactive Publication Date: 2019-02-15
谭兴江
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of the current lack of an effective preservation method for clematis germplasm resources, the present invention provides a slow-growth preservation method for test-tube seedlings of clematis germplasm, which is used to preserve wild germplasm resources of clematis germplasm and artificially cultivated high-quality seedlings. The provenance is conducive to the continuation of the genetic diversity of A. clematis, and also meets the needs of scientific development and utilization of A. clematis germplasm resources

Method used

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  • Tube seedling slow growth conservation method for anoectochilus roxburghii germplasm

Examples

Experimental program
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Effect test

Embodiment 1

[0039] From 2013 to 2017, the Fujian garden leaf clematis introduced from the Axolotlium plantation field in Anzhen, Wuxi, Jiangsu, was used as the test material, and the germplasm preservation test was carried out according to the present invention. The detailed steps are as follows:

[0040] S1. Extraction and disinfection of explants

[0041] Choose a golden clematis plant with good growth and no pests and diseases, wash the plant with running water, remove the root and leaves, and keep the stem section as an explant, first disinfect it with 70% isopropanol for 30 seconds in an ultra-clean workbench, and aseptic Rinse with water 3-4 times, then sterilize with 0.1% mercury liter + 3 drops of Tween 20 for 8 minutes, rinse with sterile water 4-6 times; finally soak in 500mg / L polyvinylpyrrolidone for 6 minutes, filter paper to absorb excess water and set aside;

[0042] S2. Inoculation and induction

[0043] Cut the sterilized explants in step S1 into 1-2cm segmented stem seg...

Embodiment 2

[0058] In order to prove the influence of the stress induction method on the effect of germplasm preservation, step S3 in Example 1 is canceled in this example, and the rest of the steps are consistent with Example 1 and will not be repeated here.

Embodiment 3

[0060] In order to prove the effect of the germplasm preservation medium of the present invention on the effect of germplasm preservation, in this example, the germplasm preservation medium in Step S5 of Example 1 is replaced with MS medium, and the remaining steps are consistent with Example 1, and will not be repeated. .

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Abstract

The invention provides a tube seedling slow growth conservation method for anoectochilus roxburghii germplasm. The method comprises the following steps: S1, material drawing and sterilization of explants; S2, inoculation and induction; S3, subculture multiplication; S4, low-temperature stress treatment of tube seedlings; S5, slow growth conservation of the tube seedlings; S6, rejuvenation of the tube seedlings; S7, rooting of the tube seedlings; S8, transplantation. The method effectively slows the growth speed of the tube seedlings by changing microenvironment conditions for the growth of germplasm materials, prolongs the subculture cycle, ensures that the germplasm preservation time reaches 3 to 4 years, is stable in inheritable character, high in germplasm survival rate and obvious in rejuvenation effect, and has important significance on further protection of the anoectochilus roxburghii resource and research, development and utilization of anoectochilus roxburghii.

Description

technical field [0001] The invention belongs to the biotechnology of medicinal plants in the field of agriculture and forestry, and in particular relates to a method for slow-growing and preserving test-tube seedlings of Aurea germplasm. Background technique [0002] Clematis ( Anoectochilus roxburghii ) that is, Mosaic orchid, also known as golden silkworm, golden thread orchid, golden stone pine, golden thread tiger head banana, golden thread into the bone, etc., is a perennial herb of the genus Orchidaceae; its leaves are round and shield-shaped , the surface is dark brown, the underside is dark red, flannelette-like is obvious, and there are golden reticular veins called "golden thread". Clematis likes to be cool and humid, and especially likes to grow in ditch edges with evergreen broad-leaved trees, stone walls, and moist areas with loose soil. It is generally distributed in hilly lands at an altitude of 300 to 1,200 meters. my country is mostly distributed in Fujian...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 谭兴江
Owner 谭兴江
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