The invention relates to a myogenin (MyoG) gene enhancer, belonging to the technical field of genetic engineering. The MyoG gene enhancer consists of regulatory elements, namely E-box, SRE, KLF3, E-box, Sp1, E-box, Sp1 and TEF-1. The MyoG gene enhancer is characterized in that the sequence of the regulatory elements of the MyoG gene enhancer is E-box, SRE, KLF3, E-box, Sp1, E-box, Sp1 and TEF-1; and the SE nucleic acid sequence of the MyoG gene enhancer is shown in Seq ID No: 1 and has the length of 147 bp. Due to an artificially-synthesized enhancer sequence, the expression of a MyoG gene can be promoted at the proliferation and differentiation stages of myoblasts, and the expression activity of the MyoG gene can be remarkably improved.