41 results about "Gene pathway" patented technology

Genes involved in double-stranded RNA interference (RNAi pathway genes) are identified and used to investigate the RNAi pathway. The genes and their products are also useful for modulating RNAi pathway activity.

The present invention concerns methods and compositions for identifying genes or genetic pathways modulated by miR-16, using miR-16 to modulate a gene or gene pathway, using this profile in assessing the condition of a patient and / or treating the patient with an appropriate miRNA.

Provided are a method and system for predicting pathways of genes that serve the same biological purpose using gene expression pattern data and protein interaction data. The method of predicting a gene pathway includes: a first step of generating partial pathways from gene expression pattern data by using a pathway extraction algorithm, and matching the partial pathways to express them as a graph; a second step of generating partial pathways from protein interaction data by using a pathway extraction algorithm, and matching the partial pathways to express them as a graph; and a third step of combining a graph made in the first step and a graph made in the second step by using a graph matching algorithm to make a combined graph.

The invention relates to methods for producing a wax ester in recombinant host cells engineered to express a thioesterase, an acyl-CoA synthetase, an alcohol-forming fatty acyl reductase, and a wax ester synthase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, recombinant host cells expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, and systems for producing a wax ester via a pathway using these four enzymes, are also provided.

The present invention is directed to methods for altering the fate of a cell, tissue or organ type by altering Notch pathway function in the cell. The invention is further directed to methods for altering the fate of a cell, tissue or organ type by simultaneously changing the activation state of the Notch pathway and one or more cell fate control gene pathways. The invention can be utilized for cells of any differentiation state. The resulting cells may be expanded and used in cell replacement therapy to repopulate lost cell populations and help in the regeneration of diseased and / or injured tissues. The resulting cell populations can also be made recombinant and used for gene therapy or as tissue / organ models for research. The invention is directed to methods for of treating macular degeneration comprising altering Notch pathway function in retinal pigment epithelium cells or retinal neuroepithelium or both tissues. The present invention is also directed to kits utilizing the methods of the invention to generate cells, tissues or organs of altered fates. The invention also provides methods for screening for agonists or antagonists of Notch or cell fate control gene pathway functions.

Recombinant yeast that are genetically modified to contain and express genes or gene pathways that produce hydroxylated and / or methoxylated flavonoids are provided. The genes or gene pathways are derived from plants, for example, from sweet basil (Ocimum basilicum) and the hydroxylated and / or methoxylated flavonoids include, for example, 6-methoxylated naringenin, 6-methoxylated luteolin and 6-methoxylated kaempferol.

The invention discloses a method for improving erythromycin yield by a saccharopolyspora erythraea SACE_7301 gene pathway. The method is characterized in that gene copy number of the saccharopolyspora erythraea SACE_7301 or gene expression quantity of the SACE_7301 is increased by a gene engineering pathway so as to obtain high-yield engineering strains of saccharopolyspora erythraea erythromycin, and yield of the erythromycin can be improved by fermenting the strains obtained by the technology.

The invention relates to a traditional Chinese medicine compound extract, and a preparation method and an application thereof. The traditional Chinese medicine compound extract is composed of ginseng and Radix Ophiopogonis. Researches show that about 90% of pancreas cancer, 50% of colorectal carcinoma, 30-40% of lung adenocarcinoma and 5-40% of leukemia are caused by ras gene over-expression, so an ras gene becomes a universally accepted target for screening antitumor drugs. Compared with traditional Chinese medicine compound extract water decoction treatment liquids, the traditional Chinese medicine compound extract obtained through the method in the invention has the advantages of low toxicity and good ras gene pathway excessive activation effect. The invention also provides a new use of the traditional Chinese medicine compound extract, an application of the extract in inhibition of ras proto-oncogene over-expression, and an application of the extract in antitumor medicines for inhibiting ras proto-oncogene mutated over-expression.