Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

An anti-tumor traditional Chinese medicine compound extract that inhibits the overexpression of ras proto-oncogene

An extract and overexpression technology, applied in the field of anti-tumor drugs, can solve the problems of poor safety and high toxicity, and achieve the effects of reducing toxicity, good effect and mortality.

Active Publication Date: 2018-05-01
LANZHOU UNIVERSITY
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On December 20, 2005, Nexavar, a drug for the treatment of advanced renal cell carcinoma approved by the US FDA, directly targets the Ras signaling pathway, which can inhibit the Raf / MEK / ERK signaling pathway and directly inhibit tumor growth; the drug targeting the ras effector pathway Kinase inhibitors such as ID1839 and OSI774, which effectively block the activity of EGFR tyrosine kinase and to some extent also inhibit other members of the ERBB protein family, but these are not drugs that directly target ras
[0009] In the process of preparing the Chinese medicine compound extract, it was found that only water decoction treatment can significantly inhibit the excessive activation of the ras gene pathway, but the toxicity is high and the safety is poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An anti-tumor traditional Chinese medicine compound extract that inhibits the overexpression of ras proto-oncogene
  • An anti-tumor traditional Chinese medicine compound extract that inhibits the overexpression of ras proto-oncogene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment one Chinese medicine compound

[0033] Materials: Ginseng and Ophiopogon japonicus were purchased from the Yellow River Market in Lanzhou.

[0034] (1) Preparation of Chinese medicine compound water decoction

[0035] Weigh 900g of ginseng and 450g of Ophiopogon japonicus; decoct in water to obtain the total extract, dissolve in water, constant volume, and finally prepare the obtained samples into 1.25mg / ml, 2.5mg / ml, 5mg / ml solutions for later use.

[0036] (2) Preparation of Chinese medicine compound extract

[0037]Weigh 900g of ginseng and 450g of Radix Ophiopogon japonicus; soak in absolute ethanol solution, filter after heating and reflux, and concentrate under reduced pressure to obtain the total Chinese medicine compound extract, dissolve the total extract in water, and set the volume to 100ml; use D101 macroporous The chromatographic column is filled with resin, pretreated and set aside. Take 20ml of the extract, put it on a D10...

Embodiment 2

[0038] Example 2 The effect of Chinese medicine compound extract on improving the toxicity of Chinese medicine compound water decoction

[0039] Ras is a small molecular protein that participates in the regulation of many important links in the development of animals. The ras signaling pathway is highly conserved from nematodes to humans. In Caenorhabditis elegans let-60 gene encodes a conserved RAS protein, which has 83% homology with human RAS protein. If the ras mutation, in Caenorhabditis elegans, it will cause the nematode to produce polyvagina, and in humans, it will cause the malignant proliferation of cells, leading to the occurrence of tumors. Therefore, the model organism Caenorhabditis elegans (Caenorhabditis elegans) Ras / MAPK signaling pathway overactivated mutants are widely used as a test group to screen anti-tumor drugs that inhibit the overactivation of proto-oncogene ras pathway.

[0040] MT2124 is a let-60 / ras overactivated C. elegans that produces a polyvag...

Embodiment 4

[0075] Example 4 The effect of Chinese medicine compound extract on Caenorhabditis elegans MT8189 (lin-15 mutation inactivation)

[0076] Experimental materials: Caenorhabditis elegans MT8189, polyvulva phenotype, genotype: lin-15b(n765)X, purchased from CGC (Caenorhabditis Genetics Center); Escherichia coli OP50, a uracil-leaking mutant strain, used as Caenorhabditis elegans food, purchased from CGC.

[0077] Concrete experimental procedure: with embodiment two.

[0078] lin-15(n765)X is located upstream of ras, and normally inhibits the expression of ras gene. Inactivation of this gene in the MT8189 nematode strain leads to the phenotype of multiple ovipositors in nematodes.

[0079]In this example, the ratio of the compound Chinese medicine extract to the mutant of Caenorhabditis elegans MT8189 (lin-15 mutation inactivation) was 100%, indicating that the compound Chinese medicine extract could not inhibit the polyvulva phenotype of lin-15(n765)X, According to the principl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a traditional Chinese medicine compound extract, and a preparation method and an application thereof. The traditional Chinese medicine compound extract is composed of ginseng and Radix Ophiopogonis. Researches show that about 90% of pancreas cancer, 50% of colorectal carcinoma, 30-40% of lung adenocarcinoma and 5-40% of leukemia are caused by ras gene over-expression, so an ras gene becomes a universally accepted target for screening antitumor drugs. Compared with traditional Chinese medicine compound extract water decoction treatment liquids, the traditional Chinese medicine compound extract obtained through the method in the invention has the advantages of low toxicity and good ras gene pathway excessive activation effect. The invention also provides a new use of the traditional Chinese medicine compound extract, an application of the extract in inhibition of ras proto-oncogene over-expression, and an application of the extract in antitumor medicines for inhibiting ras proto-oncogene mutated over-expression.

Description

technical field [0001] The invention relates to a compound Chinese medicine extract and a preparation process thereof. Specifically, the Chinese medicine compound is composed of ginseng and Ophiopogon japonicus, and specifically relates to the application of the Chinese medicine compound extract in inhibiting the overexpression of the ras proto-oncogene and in the preparation of the anti-ras proto-oncogene The invention relates to the application of antitumor drugs overexpressed after cancer gene mutation, belonging to the field of antitumor drugs. Background technique [0002] At present, malignant tumors have become the number one killer of human beings, seriously endangering human health. According to the data of the World Health Organization, the incidence of tumors in the world is 10 million people every year, and about 7 million people die. The number of cases is 2 million, and the death due to cancer is about 1.4 million. Among the 5 people who die in our country, 1 p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8968A61P35/00
Inventor 李红玉李孟惠支德娟李洋李莹辉陈晓雨
Owner LANZHOU UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com