The invention discloses a primer and probes used for full
RAS mutation detection. The primer and probes comprise a full RAS specific primer and the probes, and the full RAS specific primer and the probes comprise twenty seven
mutation types of
KRAS 2,3,4 exons and twenty three
mutation types of NRAS 2,3,4 exons. A detection kit used for full
RAS mutation detection extracts
a DNA sample and performs a PCR reaction. The specific MGB Blocker probe, the NRAS specific probe and the
KRAS specific probe and the primer are adopted and can detect
RAS mutation in
DNA in
tumor tissue; MGB Blocker is used for retarding nonspecific augmentation of NRAS and
KRAS, and sensitivity is improved and higher than 1% of ARMS; RAS
mutation can be detected in circular tumor
DNA. The primer and the probes are easy to operate, low in detection cost, capable of being popularized in a large-scale mode and high in detection speed, and the detection process can be finished after about 2 hours.