The invention discloses a kit for detecting CYP3A4*4 genetyping, belongs to the testing field of in-vitro nucleic acid, and particularly relates to a pyrophosphoric kit for detecting CYP3A4*4 genetyping. The pyrophosphoric kit for detecting CYP3A4*4 genetyping comprises uracil DNA (deoxyribonucleic acid)glycosylase, Taq polymerase, a PCR (Polymerase Chain Reaction) amplimer and a sequencing primer. The pyrophosphoric kit provided by the invention has high sensitivity and good specificity; and after simple treatment, a PCR product can be subjected to sequencing by utilizing phosphoric acid sequencing instrument, the operation is simple and convenient, the reaction time is short, the sequencing operation by adopting the pyrophosphoric kit provided by the invention has higher sensitivity when being compared with a gold-standard CE (capillary electrophoresis) sequencing operation, thus the pyrophosphoric kit is particularly suitable for mutation analysis.