49results about How to "Increase final yield" patented technology

The present invention relates to a process for preparing a biomass, such as from a microbial fermentation, for an extraction process to separate desired chemicals, nutritional products, bioactive components, proteins, carbohydrates, and lipids, from the biomass. Particularly preferred substances to extract include docosahexaenoic acid, docosapentaenoic acid, and arachidonic acid. The present invention also includes extracting the prepared biomass. Biomasses to be treated in accordance with the methods of the invention include plant, animal, and microbial biomass, particularly a microorganism such as Crypthecodinium cohnii and a fungus such as Mortierella alpina.

The invention includes methods and compositions for the production of high titer recombinant adeno-associated virus (rAAV). The disclosed rAAV are useful in gene therapy applications. Methods are based on the use of recombinant herpes virus vectors and result in highly efficient production of rAAV.

The invention discloses a solar pyrolysis reactor which is composed of a pyrolysis system, a power system, a transfer system, a monitoring system and an auxiliary system. Groove-type parabolic reflectors are utilized to focus direct light of sun, and a vacuum heat collection tube is used as an absorber; and the novel biomass pyrolysis reactor combines the solar direct metal-glass vacuum heat collection pipe and the biomass pyrolysis reactor. The solar energy is used as the heat source to directly pyrolyze the biomass, thereby generating biological combustible gas, biochar, wood vinegar and wood tar. The solar pyrolysis reactor is applicable to the fields of new rural community energy supply and modern ecological agriculture.

The present invention is process of extracting rhamnolipid as biosurfactant. The process includes the following steps: centrifuging rhamnolipid fermenting liquid at 4 deg.c in 8000 rpm to eliminate thallus, adding ammonium sulfate in 60-120 mg / L, stilling in a fridge at 4 deg.c for 10 hr, and centrifuging to eliminate protein precipitate; diluting with icy salt solution, eliminating protein precipitate to obtain supernatant, stilling in a fridge at 4 deg.c for residual grease to demulsify and agglomerate in the surface of the supernatant, skimming the grease; regulating the solution pH to below 2, stilling in a fridge at 4 deg.c for 10 hr; centrifuging at 4 deg.c and 8000 rpm for 30 min and freeze drying the collected yellowish precipitate to obtain rhamnolipid product. The technological process of the present invention features low production cost and environment friendship.

The invention discloses a method for culturing South African sugpo prawns by a higher-place pond, high salinity and high density, and belongs to the technical field of marine culture. The method includes South African sugpo prawn seed workshop temporary culture, higher-place pond culture management, scallop cage throwing in the higher-place pond and fishing by stages. By combination of factory-like workshop culture modes, the South African sugpo prawns are salinized and temporarily cultured, microecological bacteria are reasonably used in the culture process, waste scallop cages are thrown into the higher-place pond, the sugpo prawns are fished by stages at a late culture stage, the South African sugpo prawns can adapt to high-salinity water, water quality can be ensured at the late high-density culture stage, and the method is of great significance for increase of survival rate at an earlier stage, guarantee of culture water quality and yield improvement.

A test kit for testing a chip subassembly and a testing method by using the same is provided. The chip subassembly includes at least two stacked chips each having a number of electric contacts is provided. The test kit includes a test socket and a test plate. The test socket is configured to electrically engage the electric contacts on a first side of the chip subassembly. The test plate has at least a number of first probes configured for electrically engaging the electric contacts on a second side of the chip subassembly. At least one of the test socket and the test plate has a number of second probes for electrically connecting the test socket and the test plate.

The invention discloses a bacterial strain for preparing highly effective capsula glucoprotein immuno-modulatory agent, its selection and use thereof, which relates to a strain of K. peneumoniae and its screen selecting method, and the method for preparing capsula glucoprotein by using the strain, wherein the K. peneumoniae capsula glucoprotein immuno-modulatory agent is prepared through the steps of strain screening, culture, cracking, crude extracting and purification, thus overcoming the problem of drug tolerance for antibiotics when used for treating infection.

The invention discloses a method for treating desert cistanche seeds. The method is characterized in that the raw material medicines comprise an inductive agent, 1-chloromethylsilatrane, Vc and a gibberellin solution. The method has the beneficial effects that the medicine seeds are soaked by using the inductive agent, and anti-freezing, drought resistance, water resistance, cold resistance, light resistance and antioxidant genes of plants are integrated, so that roots of plants are deep and are shortened, disease and cold resistance and insect resistance and repelling are realized; the emergence rate of the cistanche seeds is increased, the root system is prompted to be developed, the root capacity is increased, and problems of low inoculation rate and low benefit in desert industrial and scale development of cistanche in different places at present are effectively solved.

The invention discloses an extraction method of lincomycin. A lincomycin fermentation solution is filtered through a ceramic film with pore diameters ranging from 0.01 mu m to 0.1 mu m after the pH (potential of hydrogen) of the lincomycin fermentation solution is adjusted to 2.0-4.5, then an acid aqueous solution with the pH ranging from 2.0 to 4.0 is used for washing the ceramic film repeatedly, filter liquor is discarded, a hydrochloric acid aqueous solution with the pH ranging from 8 to 10 is used for washing the ceramic film repeatedly, purified water is added for dissolution, and a lincomcin aqueous solution is obtained. Clear filter liquor is obtained after filtration of the ceramic film, a destaining solution and a desorbed solution can be both used for production of the next batch of lincomycin, the utilization rate of raw materials can be increased, meanwhile, the ultimate yield of the next batch of products can be further increased, and the extraction method has the advantages that a precipitation method is simple and convenient and a technological process is applicable to large-scale production.

The invention relates to the technical field of breeding, in particular to a method suitable for breeding sandworm in Danzhou areas. The method comprises the following steps: constructing a water storage pond, a sand filtration pond, a sedimentation pond, an oviposition pond, a hatching pond, a benthic cultivation pond, an intermediate cultivation pond and a mud flat breeding farm; releasing parent worms; performing feeding; performing water quality optimization and water temperature adjustment; producing trochophore larvae; performing trochophore larvae cultivation, performing benthic seedling cultivation; performing intermediate seedling cultivation; and releasing seedlings to the mud flat breeding farm. The oviposition, hatching and growth of the sandworm are artificially interfered, and the sandworm is released into the mud flat breeding farm until the sandworm is close to an adult worm, so that the physical fitness of the worm body is ensured, the sandworm density on the mud flatis ensured, and the use efficiency of the mud flat is maximized, thereby the final yield of the sandworm is greatly improved.

The invention relates to the field of gene engineering and cytology, in particular to an AAV receptor knocking out method, an HEK293 cell strain with an AAV receptor being knocked out and an application. The knocked-out AAV receptor is KIAA0319L. The knocking out method comprises the following steps: establishing a sgRNA carrier of a targeted AAVR genome sequence; carrying out transfection for a 293 T-cell, and culturing to obtain each clonal cell; carrying out Suveyor gene mutation analysis test; selecting AAVR mutant monoclone; then selecting an AAVR mutant cell strain with high yield of AAV; identifying a mutation condition of other potential mutant site; and enlarging the cell strain without other mutant sites, and establishing a library for production. The final yield of the AAV produced by the HEK293 cell strain of the knocked-out AAV receptor is about 50 percent higher than the yield of the conventional HEK293.

The invention belongs to the field of fermentation engineering, and discloses a composite extracting agent and a method for promoting production of monascus pigments by using of the composite extracting agent, wherein the method comprises the steps of: inoculating a monascus liquid seed in the logarithmic growth phase into a fermentation medium according to the inoculum size of 3%-8% by volume, adding peanut oil into the fermentation medium according to the amount of 70 mL / L fermentation liquid to 180 mL / L fermentation liquid; performing shake-flask culture at temperature of 27 DEG C to 33 DEGC; adding non-ionic surfactant Span80 into the fermentation system containing the Monascus liquid seed and the peanut oil according to the amount of 2.8 g / L fermentation liquid to 3.4 g / L fermentation liquid, performing shake-flask culture again, and the like. The final yields of red, orange and yellow pigments in the fermentation liquid with the composite extracting agent added are increased by76%, 85% and 89%, respectively, compared with the control group.

The invention relates to a method for improving the activity of anaerobic bacteria for producing butanol, and solves the problems of an existing butanol producing technology that the substrate cost is high; the butanol producing efficiency is low; the content of by-products is high. When butanol producing bacteria are fermented to the logarithmic phase, a certain concentration of butyric acid solution and buffer agents are externally added; after uniform mixing, shaking culture is continuously performed under the constant temperature condition; fermentation liquid containing rich butanol is obtained. The butyric acid is externally added, so that more fermentation substrates enter to the butanol synthesis metabolic pathway; the generation of by-products such as acetic acid and butyric acid of a fermentation system is reduced; the butanol yield is greatly improved; the proportion of the butanol in the fermentation liquid is increased. The two substances of butyric acid and buffer agents are simultaneously selected to be added in the logarithmic phase, so that the bacterium fermentation activity can be simulated; the pH of the fermentation system can also be stabilized not to generate an acid collapse phenomenon. The method has the advantages that the final yield of the butanol is improved; the butanol synthesis rate is also greatly improved; important significance is realized in the aspects of energy sources and environment.

The invention discloses a construction method of recombinant clostridium acetobutylicum and application of the recombinant clostridium acetobutylicum in preparation of butanol by fermenting hemicellulose. The recombinant clostridium acetobutylicum is constructed by overexpression of xylanase genes in clostridium acetobutylicum. Compared with the prior art, the invention has the following advantages: (1) the xylanase gene is overexpressed in clostridium acetobutylicum to successfully express the xylanase, and the xylanase has high solubility and can be completely secreted out of cells, therebyeffectively enhancing the hemicellulose degradation capacity of clostridium acetobutylicum; (2) the biological process of preparing butanol by fermentation by taking hemicellulose as a unique carbon source is realized, the cost of preparing butanol is greatly reduced, and the feasibility of converting renewable biomass energy into biofuel is further verified; and (3) compared with wild bacteria, the recombinant clostridium acetobutylicum constructed by the invention has the advantage that the butanol yield is increased from 1.25 g / L to 4.03 g / L.

The invention discloses a recombinant Escherichia coli. The strain contains a 2[4Fe4S]ferredoxin gene with a nucleotide sequence as shown in SEQ ID NO.1, and is named as Escherichia coli pCodonPlus / pRKISC / pET51b-2[4Fe4S]Fd which is obtained by the steps of connecting the 2[4Fe4S]ferredoxin gene into a pET51b carrier to prepare a recombinant plasmid named as pET51b-2[4Fe4S], and transferring the prepared recombinant plasmid to E.coli C41(DE3) containing pCodonPlus and pRKISC plasmids. The invention also discloses application of the recombinant Escherichia coli to fermentative production of 2[4Fe4S]ferredoxin. Experiments prove that each litre of fermented cultures can be used for generating 3.5mg of purified 2[4Fe4S]ferredoxin, and the yield is far higher than 0.5mg of 2[4Fe4S]ferredoxin purified from wild bacteria, and therefore, the recombinant Escherichia coli disclosed by the invention has a favorable application prospect in industrial production.

The invention discloses a greenhouse muskmelon planting method which comprises the following step of 1, seed pretreatment, specifically, airing muskmelon seeds in the sun for 2-4 h, turning over onceevery 1 h, soaking the seeds with warm water at 55 DEG C to 60 DEG C, pouring the seeds into water, continuously stirring with a glass rod, continuously adding hot water, keeping the temperature at 55DEG C and 60 DEG C for 20-30 min, continuously stirring until the water temperature is reduced to 28 DEG C and 30 DEG C, keeping the temperature for soaking for 4-6h, washing the soaked muskmelon seeds with clear water, wrapping the muskmelon seeds with clean wet gauze, sleeving the wet gauze with a layer of plastic bag, then carrying out water bath at 25-28 DEG C, and finishing pretreatment whentwo thirds of the muskmelon seeds are whitened. Muskmelons planted through the method are high in germination rate, few in disease and large in yield, meanwhile, an adopted watering device is simpleand convenient to use, the watering direction and speed can be adjusted, muskmelon seedlings can fully receive water, and growth conditions are optimized.

The invention discloses an integrated control method of lobster diseases, comprising the steps of (1) drying a pond in autumn, sterilizing, and planting Elodea nuttallii; (2) feeding lobsters with prepared herbal formula feed; (3) keeping cultivation water clear and transparent, with pH maintained to acidic; (4) keeping Elodea nuttallii in the culture pond to grow vigor; (5) purifying water with quick lime each year, once 7-10 days, 3-5 times in continuity; compared with the prior art, the integrated control method of lobster diseases provided herein has the advantages that the treatment measures herein are complementary and indispensable, the special herbal feed is used herein, the materials are strictly matched, main and auxiliary materials are clarified, the ingredients are synergic, feeding ability and immunity of lobster can be effectively improved, disease occurrence is decreased, and the yield is increased.

The embodiment of the invention provides a crop growth state determination method, a crop operation method and a related device. The method comprises the steps that the average growth height and the average growth number of crops in a measurement area within a preset time period are acquired; wherein the average growth number corresponds to a preset growth interval; wherein the growth interval represents a standard growth height range corresponding to the average growth number; and when the average growth height is not in the preset growth interval, it is determined that the growth vigor of the crops in the measurement area is abnormal. By comparing the obtained average growth height of the crops with the height in the preset growth interval, when it is determined that the average growth height is not in the growth interval, it is determined that the growth vigor of the crops is abnormal, so that the growth vigor information of the crops can be quickly and effectively obtained, and theplanting efficiency is improved while the workload of manual labor is reduced.

The present disclosure provides a zeolite-based compound having a high crystallinity, a method for producing the zeolite-based compound, and a method for producing methyl acetate using the zeolite-based compound. The zeolite-based compound includes a zeolite-based core; and a surface-portion formed on at least a portion of a surface of the zeolite-based core and made of ferrierite.

The invention relates to novel tag protein for protein enrichment expression and intracellular localization as well as application thereof. The tag protein is derived from membrane protein-reflectin A1(RA1) of a squid iridophore. The application of the novel tag protein for protein enrichment expression and intracellular localization comprises the following steps: (a) providing a construction object, wherein in an open reading frame of a gene expression vector, the construction object, from 5' to 3' segments, sequentially comprises the following operable connecting components: an encoding geneof eGFP and an encoding gene of the RA1; (b) transferring the construction object in (a) into a host cell so as to express eGFP and RA1 tags; (c) providing a group of recombinant protein formed by the eGFP and RA1 tags, wherein the recombinant protein contains functional areas of the eGFP and the RA1, and the eGFP exerts under the action of the RA1 functional area; and (d) performing intracellular enrichment and spatial orientation to be favorable for the subsequent separation and purification.

The invention discloses a compound agent for preventing and controlling premature senility of cotton, which is composed of solid powder and a liquid preparation, wherein the solid powder comprises borax, urea and potassium dihydrogen phosphate; and the liquid preparation is an ethanol solution of brassinolide. The compound agent can improve the biological activity of the cotton plant and increase the nutrient supply in the cotton growth process, thereby maximally solving the problem in preventing and treating premature senility of cotton and obviously enhancing the ultimate yield of cotton.

The invention discloses a method for comprehensive prevention and control of crayfish diseases, which comprises the following steps: (1) drying ponds in autumn, disinfecting and planting Elodea; (2) feeding crayfish with prepared Chinese herbal medicine formula feed; (3) maintaining The aquaculture water is clear and transparent, and the pH is kept at an acidic level; (4) Keep the Elodea medicine in the aquaculture pond growing vigorously; (5) Purify the water quality with quicklime every year, once every 7-10 days, and use it continuously for 3-5 times . Compared with the prior art, the present invention provides a comprehensive prevention and control method for crayfish diseases, in which the various treatment measures complement each other and are indispensable, and in the specific Chinese herbal medicine feed used, each raw material is strictly compatible, the main and auxiliary components are distinct, and each component The synergistic effect and mutual synergy can effectively improve the feeding ability and immunity of crayfish, reduce the incidence of diseases, and increase production.