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Composite extracting agent and method for promoting production of monascus pigments by using of composite extracting agent

A compound extraction agent and monascus pigment technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve difficult problems, increase the production of monascus pigment, etc., achieve economic benefits and good application prospects , the effect of increased pigment production

Inactive Publication Date: 2018-07-06
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0007] At present, the existing methods such as strain screening and optimization of fermentation conditions have reached the bottleneck, and it is difficult to further increase the yield of red yeast rice pigments, and then seek a new type of fermentation method, that is, a two-liquid phase coupling extraction fermentation system
The method that the non-ionic surfactant Span80 and peanut oil are mixed according to a certain ratio and used as an extractant for the production of monascus pigment has not yet been reported

Method used

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  • Composite extracting agent and method for promoting production of monascus pigments by using of composite extracting agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Slant Medium: Potato Dextrose Agar Medium (PDA)

[0050] Seed liquid medium: glucose 20-40g / L, soybean powder 10-30g / L, glycerin 60-80g / L, peptone 5-15g / L, MgSO 4 ·7H 2 O 0.5-3g / L, KH 2 PO 4 1-4g / L, NaNO 3 1-3g / L.

[0051] Fermentation medium: glucose 30-60g / L, soybean powder 15-35g / L, glycerin 70-100g / L, peptone 5-20g / L, MgSO 4 ·7H 2 O 0.5-2g / L, KH 2 PO 4 1.5-5g / L, NaNO 3 1-5g / L, ZnSO 4 ·7H 2 O 1-4g / L.

[0052] Measurement and calculation of the color value of the three pigments: take a certain volume of fermentation broth, centrifuge, filter, dilute the filtrate to an appropriate multiple with deionized water, and use a spectrophotometer to measure at wavelengths of 390-420nm, 430-480nm, and 490-520nm respectively The OD value, the OD value under the three wavelengths multiplied by the dilution factor is the color value of the extracellular yellow, orange and red three pigments. After centrifugation, the bacterial cell precipitate is mixed with 50-90% e...

Embodiment 2

[0060] It is the same as the strain cultivation and fermentation process steps of the above-mentioned Example 1, the difference is that the fermentation medium is prepared according to the following formula, the basic medium is rice flour 30-60g / L, glycerol 70-100g / L, peptone 5- 20g / L, MgSO 4 ·7H 2 O 0.5-2g / L, KH 2 PO 4 1.5-5g / L, NaNO 3 1-5g / L, ZnSO 4 ·7H 2 O 1-4g / L. The extraction phase is still the nonionic surfactant Span80 and peanut oil, and the addition time is also the same as in Example 1. After 5-10 days of fermentation under the same fermentation conditions as in Example 1, the total color values ​​of red, orange, and yellow three pigments were measured to be 180-190U / mL, 160-170U / mL, and 180-190U / mL, respectively.

Embodiment 3

[0062] The steps and fermentation medium formula are the same as those of the above-mentioned Example 1, except that the strains are different. The strain Monascus RP2 preserved in this laboratory was selected.

[0063] The fermentation steps, fermentation process and conditions are the same as those in the first embodiment above. After the fermentation is finished, the total color values ​​of red, orange and yellow three pigments measured by ultraviolet spectrophotometer are 150-160U / mL, 140-150U / mL and 140-150U / mL respectively.

[0064] It can be seen from the above examples that the composite extractant combined with nonionic surfactant Span80 and peanut oil has good biocompatibility and pigment extraction ability for Monascus. The invention can efficiently produce the monascus pigment, and has important practical value and economic value in the production field of the monascus pigment.

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Abstract

The invention belongs to the field of fermentation engineering, and discloses a composite extracting agent and a method for promoting production of monascus pigments by using of the composite extracting agent, wherein the method comprises the steps of: inoculating a monascus liquid seed in the logarithmic growth phase into a fermentation medium according to the inoculum size of 3%-8% by volume, adding peanut oil into the fermentation medium according to the amount of 70 mL / L fermentation liquid to 180 mL / L fermentation liquid; performing shake-flask culture at temperature of 27 DEG C to 33 DEGC; adding non-ionic surfactant Span80 into the fermentation system containing the Monascus liquid seed and the peanut oil according to the amount of 2.8 g / L fermentation liquid to 3.4 g / L fermentation liquid, performing shake-flask culture again, and the like. The final yields of red, orange and yellow pigments in the fermentation liquid with the composite extracting agent added are increased by76%, 85% and 89%, respectively, compared with the control group.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a compound extractant and a method for promoting the production of monascus pigment by using the compound extractant. Background technique [0002] Monascus pigment is a safe natural pigment, which is relatively stable to acid-base, oxidant and reducing agent. It has the characteristics of good thermal stability, strong protein coloring power, soft tone, stable pH and anti-oxidation. The color agent nitrite is used in the coloring of meat products, puffed food, aquatic products and seasoning cans, so the application of monascus pigment has a huge market prospect. [0003] At present, the production method of monascus pigment is mainly liquid fermentation method, but this traditional liquid fermentation method faces two problems: the negative feedback inhibition caused by the high concentration of the product in the cell during the fermentation process; Pigment...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/645
CPCC12P1/02C12N1/145C12R2001/645
Inventor 杨雪莲曹雁平王成涛董晔
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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