45 results about "Protein enrichment" patented technology

The invention relates to a multifunctional double-layer core-shell structure magnetic nano particle. In the invention, a magnetic nano particle with a particle size of 1-300 nm is used as a core and coated with a double-layer shell consisting of a SiO2 layer with a thickness of 1-200 nm and a hydrolyzed silane coupling agent layer is 1-100 nm thick and comprises one or more multifunctional groups; the particle size and the shell layer thickness can be controlled through regulating the volumes, the weight ratios and the reaction time of the magnetic core, a silicon dioxide precursor, a silane coupling agent and a catalyst in a preparation process; the total particle size of the nano particle can be as small as 5-50 nm and as large as 700-800 nm; the nano particle can have superparamagnetism, paramagnetism and ferromagnetism according to the change of the magnetic core particle size; and one or more bioactive molecules can be connected into the shell layer of the magnetic nano particle or to the surface of the shell layer through a chemical method or a physical method. The invention also provides a preparation method of the multifunctional double-layer core-shell structure magnetic nano particle and application thereof. The particle preparation method has the advantages of simplicity, moderate condition, low cost and easy realization of industrial production. The nano particle can obtain different functions through connecting different bioactive molecules and can be applied to the fields of protein enrichment, biological detection, separation and purification, targeted drug carriers, cell imaging and medical imaging.

The invention discloses an integrated proteomic sample pretreatment platform based on an SCX (strong cation exchange)/SAX (strong anion exchange) mixed filling material and application thereof. The proteomic sample pretreatment platform comprises a pipette gun head (1), strong cation exchange resin and strong anion exchange resin mixed filling materials (2) and a solid phase extraction film (3), wherein the solid phase extraction film (3) is loaded in the lower end of the pipette gun head (1); the strong cation exchange resin and strong anion exchange resin mixed filling materials (2) are loaded in the lower end of the pipette gun head (1), and are positioned above the solid phase extraction film (3). The proteomic sample pretreatment platform has the advantages that the steps of sample preenrichment, reduction, alkylation and enzymolysis are completed on the mixed ion exchange filling materials; a reactor is characterized in that the used mixed ion exchange filling materials can realize the protein enrichment at any pH values; in the enzymolysis pH replacement process, the protein loss is little; trypsinase can move back and forth between the two kinds of filling materials; the enzymolysis efficiency is higher.

The invention relates to a sugar protein enrichment purification method, according to the method, hydrazide chemical method for high selective sugar protein enrichment and a centrifugal ultrafiltration device for efficient purification are integrated, hydrazide sugar protein enrichment process can be completed in the centrifugal ultrafiltration device, the whole sugar protein enrichment process can be completed in situ, the sample loss can be effectively reduced, at the same time, by use of the centrifugal ultrafiltration device, a solubilizing agent can be applied in the enrichment process so as to realize sugar protein simultaneous enrichment in soluble protein and / or insoluble membrane protein; by use of the advantages of effective solvent replacement of the centrifugal ultrafiltration device, various types of elution solutions can be introduced into the sugar protein enrichment process to complete effective removal of non sugar protein so as to enhance the selectivity of sugar protein enrichment.

The invention belongs to the technical field of glycopeptide/glycoprotein enrichment materials and analysis, and relates to a sugar chain releasable glycosylated peptide fragment/protein enrichment material based on a hydrazide strategy, a preparation method of the material and an application of the material. The surface of a matrix micro-sphere is bonded with a hydrazide compound containing disulfide bonds to form a functional material with a hydrazide group on the surface, wherein the functional material can be used for glycopeptide enrichment. The disulfide bonds are led to the surface of the material to realize sugar chain releasable enrichment of glycopeptide/glycoprotein, sugar chains are in covalent binding, and the material has the advantage of high enrichment efficiency and overcomes the shortcoming that the sugar chains are not easily released in a traditional hydrazide method and cannot be used for O-glycosylated peptide fragment/protein enrichment. The sugar chain releasable enrichment material is easy to prepare and good in enrichment selectivity, is widely applicable to N-glycosylated and O-glycosylated peptide fragment/protein enrichment and has a high practical value in the fields of glycoproteomics and the like.

The invention relates to the field of drug target protein enrichment, separation and detection, and discloses a flavone glycoside functionalized magnetic nanometer affinity probe, and further providesa preparation of the flavone glycoside functionalized magnetic nanometer affinity probe, applications of the flavone glycoside functionalized magnetic nanometer affinity probe in the drug target protein capture and/or identification of cells, and a method for capturing an intracellular target protein through the flavone glycoside functionalized magnetic nanometer affinity probe, wherein the flavone glycoside functionalized magnetic nanometer affinity probe comprises magnetic nanoparticles, a coupling structure and a flavone glycoside compound supported on the surface of the magnetic nanoparticle, one end of the coupling structure is coupled to the surface of the magnetic nanoparticles, and the other end of the coupling structure is coupled to the flavone glycoside compound. With the flavone glycoside functionalized magnetic nanometer affinity probe of the present invention, the high-specificity capture can be achieved only with a small amount of the whole protein extraction liquid.

The present invention relates to a novel silica gel material capable of recognizing N-phosphopeptides and proteins under neutral conditions and application thereof in N-phosphopeptide and protein enrichment. Non-porous silica microspheres are prepared by a seed growth method, a core-shell microsphere initial layer is formed by a template-guided dissolution and re-deposition method, and finally sub-2-micron core-shell silica gel with vertical pores can be obtained by acid reflux. N-tert-butyloxycarbonyl-L-tyrosine and N, N'-dipicolylamine are reacted to form a support molecule, and a phosphaterecognition functional molecule is formed by complexation of a metal ion on the support molecule. Finally, the novel phosphoric acid recognition functional silica gel material is obtained by covalently bonding the phosphoric acid recognition functional molecule with the sub-2-micron core-shell silica gel having the vertical pores through an amide bond. The novel phosphoric acid recognition functional silica gel material can rapidly and specifically enrich the N-phosphopeptides and proteins in a neutral buffer system.

The invention discloses a pretreatment method, a preservation method, an automatic treatment system and a detection method of a urine sample, and relates to the technical field of biological detection.The pretreatment method comprises the steps that the urine sample subjected to protein splitting is subjected to reductive alkylation treatment, and then protein enrichment and enzymolysis are conducted; in the protein enrichment step, a PVDF (Polyvinylidene Fluoride) filter plate is adopted to carry out protein enrichment on the sample subjected to reductive alkylation treatment. The invention further provides an automatic urine sample treatment system and an automatic sample treatment method. The treatment system greatly reduces the labor intensity of people, facilitates the improvement of the treatment efficiency of urine sample treatment, meets the high-flux and automatic proteomics pretreatment requirements, and adapts to the reproducibility and flux of the current clinical requirements.

The invention discloses a protein enriching device. The protein enriching device mainly comprises a shell, a water drawing pump and a foam separation device, wherein a stirring device, a centrifugingdevice and a ball milling device are arranged in the shell and are used for extracting protein; the foam separation device is used for enriching the protein. Through the protein enriching device, liquid supernatant can be obtained by only adjusting a control device and a driving device to crush and centrifuge cells in raw liquid; the process is simple; the manpower and the time are saved; meanwhile, protein enriching liquid obtained by a foam separation method is excellent in effect, simple in treatment process, high in treatment capacity and low in cost and low in energy consumption.

The present invention relates to a method for protein enrichment of a biomass of red unicellular algae such as Galdieria and to the biomass thus obtained.