34results about How to "Efficient enzymatic hydrolysis" patented technology

The invention discloses a method for efficiently hydrolyzing lignocellulosic biomass and synchronously preparing multi-component liquid glucose and lignin. The method comprises the steps that: the lignocellulosic biomass through physical crushing is added with phosphoric acid to perform acid hydrolysis; then organic solvent is added to extract the lignin, the layering and the phase separation areperformed, then the lignin is extracted out while the organic solvent is reclaimed under the condition of pressure reduction and distillation; phosphoric acid can be reclaimed through steps such as neutralization, filtration, acidification and so on, hemicellulose hydrolyzed liquid glucose is obtained at the same time; and the remained cellulose undergoes the zymohydrolysis to prepare cellulosic hydrolyzed liquid glucose. The method can separate lignin, hemicellulose and cellulose, remarkably decreases the degree of crystallinity of the cellulose hydrolyzed by phosphoric acid, and remarkably improves the zymohydrolysis efficiency; and the prepared hydrolyzed liquid glucose does not contain fermentation inhibitors. The method has mild treatment conditions, simple process and less side reactions; the phosphoric acid and the organic solvent can both be reclaimed and circularly used; and the method is environment-friendly, and has broad social and economic benefits.

The invention discloses a method of processing a lignocellulosic material using an alkaline delignification agent. The present invention relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. In particular, the present invention relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis and an alkaline delignification step.

The invention discloses a method for carrying out enzymatic hydrolysis. The method comprises the following steps: adding water into protein, evenly stirring, transferring the mixture to an electrophoresis tank, carrying out electrophoresis under a voltage of 80 to 120 V, collecting the protein solution after electrophoresis, dissolving the protein by an enzymatic hydrolysis buffer solution containing trifluoroethanol, subjecting the protein to reduction and alkylation treatments, adjusting the pH value of processed protein to 7.5-8.5, then adding trypsase accounting for 90 to 98% of the protein solution, and carrying out enzymatic hydrolysis to obtain polypeptide; wherein the work electrode in the electrophoresis tank is modified by oxidation-reduction enzyme and Zn-Al layered double hydroxides. The provided method can obtain peptides suitable for liquid chromatogram-mass spectrum (LS-MS) analysis, and provides stable and reliable samples for proteomics analysis.

Lignocellulosic biomass can be fractionated for the purpose of increasing cellulose purity in the pulp, increasing native lignin content of the isolated lignin, and improving cellulose hydrolysis, by performing the steps of: (a) extracting the biomass with an extracting liquid comprising at least 20 wt % of a first organic solvent at a temperature below 100° C.; (b) treating the extracted biomass with a treatment liquid comprising a second organic solvent selected from lower alcohols, ethers and ketones, optionally water and optionally an acid, at a temperature between 120° C. and 280° C., and, optionally: (c) subjecting a cellulose-enriched product stream resulting from step (b) to enzymatic hydrolysis. The first and second organic solvent may be different or the same; in particular they comprise ethanol or acetone.

The invention discloses a method for producing ethanol by semi-synchronous enzymatic hydrolysis and fermentation of defatted peanut powder prehydrolyzate and pretreated solid substrate. The method firstly performs prehydrolysis on defatted peanut powder, including ball milling treatment, enzymatic hydrolysis, separation and other processes; Finally, the defatted peanut powder prehydrolyzate was mixed with the pretreated solid substrate and ultrasonically treated; finally, after adding the pretreated solid substrate, cellulase, cellobiase and yeast in batches, the defatted peanut powder prehydrolyzate Semi-synchronous enzymolysis and fermentation with pretreated solid substrate to produce fuel ethanol. The present invention extracts peanut protein, monosaccharides or oligosaccharides from cheap defatted peanut powder. Peanut protein can effectively shield the lignin deposited on the surface of the pretreated solid substrate and reduce the amount of cellulase. The sugar can increase the concentration of fermentable sugar, increase the concentration of fuel ethanol in the fermentation broth, and achieve the purpose of effectively reducing the production cost of fuel ethanol.