A kind of preparation method of fish protein peptide

Abstract

The invention relates to the field of biotechnology, and specifically discloses a method for preparing fish protein peptides, including a raw material preparation step, the raw material includes marine low-value small miscellaneous fish and aquatic product leftovers; a raw material crushing step; a fish paste enzymatic hydrolysis step: Transfer the fish paste in the enzymolysis tank to the enzymolysis tank, add water to the enzymolysis tank, the mass ratio of water and fish paste is 1:1, mix the water and fish paste to form a mixture A, and the enzymolysis temperature is 50-70°C, The pH during enzymolysis is 7.0, and the enzymolysis time is 2-6h; the step of inactivating the enzyme; the step of preparing the small peptide solution; the step of spray drying the enzymolysis solution; the step of microbial fermentation: mixing rice into the solid matter in the step of preparing the small peptide solution Aspergillus, forming a mixture C, the moisture content in the mixture C is 30-60%, the temperature is 25-45°C, and the fermentation time is 16-28h, forming a mixture D; a drying step; a mixing and crushing step. This scheme can extract protein peptides from low-value small miscellaneous fish and aquatic product waste, thereby reducing the processing cost of protein peptides.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing fish protein peptides. Background technique [0002] Due to the rapid development of aquaculture and special breeding in China, the demand for animal protein feed is growing rapidly. Animal protein feed has the advantages of high protein content, high animal meat yield, and easy matching with other ingredients. However, the fish meal produced in my country is generally high-grade. The price of fishmeal is relatively low, and the processing technology is backward, resulting in the low price of fishmeal. Some fishmeal with high protein content, such as special meal for prawns and special meal for soft-shelled turtle, have a serious problem of relying on imports. [0003] At present, the main economic fish resources in the ocean are withered, and the low-value small trash fish are insufficiently caught and processed due to their various shortcomings. L...

AI Technical Summary

Problems solved by technology

[0005] At present, there are many methods for preparing bioactive peptides, the common ones are enzymatic hydrolysis and microbial methods, but due to the high cost of external enzymes in the enzymatic hydrolysis method, the enzymatic hydrolysis time for preparing small peptides by the microbial method is too long, the cost is high, and conventional methods are used. After protease enzymatic hydrolysis or microbial hydrolysis, the amount of protein hydrolyzed in raw materials can only reach 26%, and the content of polypeptides below 3000Da is only 83%. This hydrolysis efficiency and degree of hydrolysis are difficult to achieve industrial production.

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