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Method for producing ethanol by semi-synchronous enzymolysis and fermentation of degreased peanut powder prehydrolysate and pretreated solid matrix

A technology of degreased peanuts and pre-hydrolyzate, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of increasing the production cost of lignocellulosic fuel ethanol, increasing the steps of pretreatment, etc., and achieve the promotion of commercial The effects of promotion and reduction of production costs

Active Publication Date: 2020-04-10
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although fed-batch can partially overcome the adverse effects of mass and heat transfer, the patent still deals with the adverse effects of lignin
In order to overcome the ineffective adsorption of lignin to cellulase, after steam explosion pretreatment, the patent further uses alkaline hydrogen peroxide post-treatment to further remove lignin from the pretreated solid matrix. Although a certain effect has been achieved, it increases pretreatment step, and new effluent
In addition, alkaline hydrogen peroxide post-treatment cannot completely remove the lignin in the pre-treated solid substrate. In order to improve the efficiency of subsequent enzymatic hydrolysis and fermentation, the amount of enzyme added in this patent is relatively high. When the ethanol concentration reaches 65g / L When above, the dosage of cellulase reaches more than 30 FPU / g dextran, which will significantly increase the overall production cost of lignocellulosic fuel ethanol

Method used

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  • Method for producing ethanol by semi-synchronous enzymolysis and fermentation of degreased peanut powder prehydrolysate and pretreated solid matrix
  • Method for producing ethanol by semi-synchronous enzymolysis and fermentation of degreased peanut powder prehydrolysate and pretreated solid matrix
  • Method for producing ethanol by semi-synchronous enzymolysis and fermentation of degreased peanut powder prehydrolysate and pretreated solid matrix

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Preparation of bamboo pretreatment solid substrate:

[0051] First, add 10g of bamboo powder that has passed through a 40-mesh sieve into a microwave reaction tank with a polytetrafluoroethylene lining, add 150g of benzenesulfonic acid in water, the mass percentage of benzenesulfonic acid in the pretreatment liquid is 80%, and seal the microwave After the reaction tank was treated at 95°C for 30 minutes, after the pretreatment was completed, the microwave reaction tank was taken out and placed in cold water to cool to room temperature, and then solid-liquid separation was carried out in a Buchner funnel lined with filter paper; then, the pretreatment was washed with deionized water Treat the solid substrate to the pH of the filtrate in the range of 6.8 to 7.0, and use the cleaned pretreated solid substrate for subsequent semi-synchronous enzymatic hydrolysis and fermentation.

[0052] (2) Pre-hydrolysis of defatted peanut flour:

[0053] First, put 20g of defatted peanut ...

Embodiment 2～6

[0073] Examples 2 to 6 and Comparative Examples 2 to 4

[0074] The specific schemes and ethanol fermentation results of Examples 2 to 6 and Comparative Examples 2 to 4 are shown in Table 1 and Table 2, respectively:

[0075] Woody biomass raw materials are all 40 mesh bamboo powder or eucalyptus powder; HC, CL, CB, G and YC are the abbreviations for hemicellulase, cellulase, cellobiase, glucan and yeast cells, respectively; R is the abbreviation of pretreatment solid-liquid ratio; AASA is the abbreviation of acetic acid-sodium acetate; DPF is the abbreviation of defatted peanut powder; PS is the abbreviation of pretreatment solid substrate.

[0076] For the pretreatment of benzenesulfonic acid, hot water, dilute sulfuric acid or dilute NaOH, after the pretreatment is completed, use a Buchner funnel with filter paper for solid-liquid separation, and wash the pretreated solid substrate with deionized water until the filtrate pH is 6.8 In the range of ~7.0, the washed pretreated solid...

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Abstract

The invention discloses a method for producing ethanol by semi-synchronous enzymolysis and fermentation of degreased peanut powder prehydrolysate and a pretreated solid matrix, comprising the following steps: carrying out prehydrolysis on degreased peanut powder, including ball-milling treatment, enzyme hydrolysis, separation and other processes; then, mixing the degreased peanut powder prehydrolysate with the pretreated solid matrix, and carrying out ultrasonic treatment; and finally, adding the pretreated solid matrix, cellulase, cellobiase and yeast in batches, and carrying out semi-synchronous enzymolysis and fermentation on the degreased peanut powder prehydrolysate and the pretreated solid matrix to produce the fuel ethanol. According to the invention, peanut protein, monosaccharideor oligosaccharide is extracted from cheap degreased peanut powder; peanut protein can effectively shield lignin deposited on the surface of the pretreated solid matrix and reduce the use amount of cellulase, monosaccharide or oligosaccharide can improve the concentration of fermentable sugar and the concentration of the fuel ethanol in fermentation liquor, and the purpose of effectively reducingthe production cost of the fuel ethanol is achieved.

Description

Technical field [0001] The invention belongs to the technical field of new energy, and specifically relates to a method for producing ethanol by semi-synchronous enzymatic hydrolysis and fermentation of defatted peanut flour pre-hydrolyzed liquid and pretreatment solid substrate. Background technique [0002] In order to reduce the rapid consumption of fossil-based energy and its use to harm social and economic stability and the environment, countries around the world are gradually seeking sustainable alternative energy sources. In addition to bio-gasoline and bio-diesel, fuel ethanol can be mixed with fossil-based gasoline (E10 and E85 mixed fuels, etc.) and used as a transportation fuel, and has received widespread attention worldwide. [0003] Woody biomass has the advantages of sustainable utilization, wide distribution, large yield, and rich in carbohydrates. It has been used by researchers around the world as an ideal raw material for the preparation of fuel ethanol. However...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12R1/645
CPCC12P7/10C12P2201/00Y02E50/10
Inventor 刘婧罗小林杨光绪龚正刚黄六莲陈礼辉
Owner FUJIAN AGRI & FORESTRY UNIV
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