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Ionic liquid-complex microbial inoculum system

A compound bacterial agent and ionic liquid technology, applied in the field of garden biomass resource utilization, can solve problems such as inefficient utilization and environmental pollution, achieve recycling, solve environmental pollution problems, and achieve vitality persistence and synergy Effect

Active Publication Date: 2020-12-11
XIAN BOTANICAL GARDEN SHAANXI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to realize the recycling and reuse of garden biomass resources and solve the technical bottlenecks such as environmental pollution and low-efficiency utilization caused by traditional methods or approaches to dispose of garden biomass resources, based on the principles of green feasibility, easy operation, durable enzymatic hydrolysis and industrial application In principle, the present invention provides an ionic liquid-composite bacterial agent system to explore its application in enzymatic hydrolysis of garden biomass resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, ionic liquid [Emim]FeCl 4 Synthesis

[0041] The present embodiment provides ionic liquid 1-ethyl-3-methylimidazolium iron chloride salt ([Emim]FeCl 4 ) A preferred synthetic method. Specifically, using N-methylimidazole and ethyl chloride as raw materials, the molar mass ratio of N-methylimidazole and ethyl chloride is 1:1.1, put N-methylimidazole and ethyl chloride into a three-necked flask at 70°C Magnetically stirred in an oil bath for 24 hours to obtain a viscous crude product, which was washed with ethyl acetate, distilled under reduced pressure to remove impurities, and vacuum-dried at 50°C to obtain the off-white intermediate [Emim]Cl; the intermediate [Emim]Cl was combined with three Ferric Chloride (FeCl 3 ) at a molar mass ratio of 1:1, at room temperature (25±5°C) for 2-5 hours of displacement reaction to obtain the light green final product [Emim]FeCl 4 .

[0042] [Emim]FeCl provided by the present embodiment 4 Synthetic Method, Ionic Li...

Embodiment 2

[0043] Embodiment 2, the preparation of ionic liquid-composite bacterial agent system

[0044] The preparation of the ionic liquid-composite bacterial agent system described in this example mainly includes the ionic liquid [Emim]FeCl 4 The synthesis of microbes, the fermentation culture of microbial strains, and the formation of coupling systems.

[0045] Ionic Liquid [Emim]FeCl 4 See Example 1 for the synthesis. Those skilled in the art should know that high-purity ionic liquids usually have biological toxicity, and this biological toxicity tends to increase with the increase of the length of the cationic alkyl side chain to a certain extent. The mechanism of the alkyl chain effect is explained as follows: the longer the carbon chain, the stronger the lipophilicity of the ionic liquid, the stronger the adsorption and aggregation force on the cell membrane of the organism, and the easier it is to damage the cell membrane and inactivate the cell. In this embodiment, the aque...

Embodiment 3

[0049] Embodiment 3, the dissolution and regeneration test of ionic liquid to garden biomass

[0050] This embodiment provides different mass fractions of ionic liquid aqueous solution to the dissolution and regeneration test of garden biomass, and the test results are shown in figure 1 .

[0051] The lignocellulosic raw materials used in this embodiment and the involved tests are garden biomass, which are mainly composed of garden prunings and litter. The contents of the main structural components are 39.3% cellulose, 21.5% lignin, half Cellulose 17.6%, the basic property is organic matter 750g·kg -1 , full carbon 436g·kg -1 , total nitrogen 4.8g·kg -1 , carbon-nitrogen ratio (C / N) 9:1, pH value 7.7.

[0052] Different parts by mass of [Emim]FeCl 4 The aqueous solution was placed in a conical flask, and the 50-mesh sieved garden biomass was added to [Emim]FeCl 4 In aqueous solution, garden biomass and [Emim]FeCl 4 Set the mass ratio of the aqueous solution to 1:10, sti...

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Abstract

The invention discloses an ionic liquid-complex microbial inoculum system which comprises ionic liquid and a complex microbial inoculum. The ionic liquid and the complex microbial inoculum form a coupling system for enzymolysis of lignocellulose; the ionic liquid contains 1-ethyl-3-methylimidazole ferric chloride salt; and the complex microbial inoculum comprises fermentation liquor of wet cellulomonas, streptomyces albus, candida tropicalis and white rot fungi. In an enzymolysis test, the highest cellulase activity of enzymatic hydrolysate is 185.1 U.mL<1>, the degradation weight loss rate ofgarden biomass reaches 52.6% and is respectively increased by 59.0% and 66.5% with respect to that of a only complex microbial inoculum, and lignin content of enzymolysis residues is reduced by 35.2%. In the enzymolysis application, the enzymolysis period of the garden biomass is 18 to 26 days, the high-temperature period lasts for 12 to 21 days, the highest temperature reaches 62 to 72 DEG C, the final degradation weight loss rate is 47.2 to 54.2%, and the lignin content is reduced to 8.5 to 10.6%. The ionic liquid-complex microbial inoculum system is simple and convenient in preparation process and outstanding in enzymolysis efficiency, and can meet the social requirements of intensive and resource treatment and utilization of agriculture and forestry biomass and the development conceptof green circulation.

Description

technical field [0001] The invention belongs to the technical field of resource utilization of garden biomass, and relates to the application of lignocellulose enzymatic hydrolysis, in particular to an ionic liquid-composite bacterial agent system for efficient enzymatic hydrolysis of garden biomass and a preparation method thereof. Background technique [0002] Garden biomass is forest and grass clippings, litter, agricultural straw, etc. produced by horticultural maintenance and agricultural and forestry operations. Its main components include degradable cellulose, lignin, hemicellulose and starch, etc., and also contain some plant nutrients. Elements are important organic resources that participate in the material cycle of the ecosystem. Lignocellulose is the main component of plant tissue structure, which is composed of glucose polymers, xylosaccharides, polyglucomannose, and phenylpropane polyphenols with covalent bonds (such as hydrogen bonds, ester bonds, ethers) and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C07D233/58B09B3/00C12R1/47C12R1/74C12R1/01C12R1/645
CPCC12N1/20C12N1/16C12N1/14C07D233/58B09B3/00
Inventor 路强强陈智坤赵叶子孙晓宇邢国强
Owner XIAN BOTANICAL GARDEN SHAANXI PROV
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