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Preparation method and application of pharmaceutical-grade sparassis crispa polysaccharide capable of enhancing immunity

A technology for enhancing immunity and embroidery, applied in the field of food, can solve the problems of poor water solubility of embroidered polysaccharides, affecting the effective absorption of embroidered polysaccharides, enhancing immunity, discount of anti-tumor and hypoglycemic effect, etc. Immunity, the effect of enhancing the body's immunity

Inactive Publication Date: 2021-04-27
NINGBO XINUOYA MARINE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When hydrangea polysaccharides are used as raw materials to prepare health care products and foods for special medical purposes, due to the poor water solubility of hydrangea polysaccharides, the effective absorption of hydrangea polysaccharides by the human body is affected, so that its effects of enhancing immunity, anti-tumor, and lowering blood sugar are greatly reduced

Method used

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  • Preparation method and application of pharmaceutical-grade sparassis crispa polysaccharide capable of enhancing immunity
  • Preparation method and application of pharmaceutical-grade sparassis crispa polysaccharide capable of enhancing immunity
  • Preparation method and application of pharmaceutical-grade sparassis crispa polysaccharide capable of enhancing immunity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Follow the steps below to prepare pharmaceutical grade immune-enhancing hydrangea polysaccharides:

[0024] 1. Pretreatment of raw materials by ionic liquid / compound enzyme system:

[0025] Get the hydrangea fruiting bodies of 1kg drying, pulverize it to 800 mesh by ultrafine pulverizer; Add in 1%wt1-butyl-1-methylpyrrolidine bistrifluoromethanesulfonylimide salt ionic liquid solution of 10L and mix Heat to 85°C and stir evenly to obtain a mixed solution, cool to 45°C, adjust pH=6, and then add a compound enzyme with a mass percentage concentration of 0.2%, and the compound enzyme is a specific endocellulase in parts by weight: lysozyme =2:1, the mixed solution was stirred at 45° C. for 1.5 h for enzymolysis, and then added with a mass percentage concentration of 0.3% compound enzyme, the compound enzyme was lipase in parts by weight: bromelain=3:1, Stir the mixed solution at 40°C for 1.5 hours for enzymolysis, centrifuge the enzymolysis solution to separate the solid ...

Embodiment 2

[0034] Follow the steps below to prepare pharmaceutical grade immune-enhancing hydrangea polysaccharides:

[0035] 1. Pretreatment of raw materials by ionic liquid / compound enzyme system:

[0036]Take 1 kg of dried hydrangea fruiting body, and pulverize it to 1000 mesh by ultrafine pulverizer; add 15L of 1%wt tetrabutylphosphine tetrachloroferrate ionic liquid solution and heat to 85°C and stir evenly to obtain a mixed solution, Cool to 45°C, prepare pH=6, then add a compound enzyme with a mass percentage concentration of 0.3%, and the compound enzyme is specific endocellulase in parts by weight: lysozyme=3:1, mix the mixture at 45 Stir at ℃ for 1.5h for enzymolysis, then add a compound enzyme with a mass percentage concentration of 0.3%, and the compound enzyme is lipase: bromelain in parts by weight = 4:1, and the mixed solution is stirred at 40°C for 1.5 h for enzymolysis, centrifuging the enzymolysis solution to separate the solid from the liquid, discarding the filtrate ...

Embodiment 3

[0045] Follow the steps below to prepare pharmaceutical grade immune-enhancing hydrangea polysaccharides:

[0046] 1. Pretreatment of raw materials by ionic liquid / compound enzyme system:

[0047] Get the hydrangea fruiting bodies of 1kg drying, pulverize it to 1200 mesh by ultrafine pulverizer; Add in 1.5%wt1-butyl-1-methylpyrrolidine bistrifluoromethanesulfonylimide salt ionic liquid solution of 10L and mix Heat to 85°C and stir evenly to obtain a mixed solution, cool to 45°C, adjust pH=6, and then add a compound enzyme with a mass percentage concentration of 0.2%, and the compound enzyme is a specific endocellulase in parts by weight: lysozyme =4:1, the mixed solution was stirred at 45°C for 1.5h to carry out enzymolysis, then adding a mass percentage concentration of 0.3% compound enzyme, the compound enzyme was lipase by weight: bromelain=4:1, Stir the mixed solution at 40°C for 1.5 hours for enzymolysis, centrifuge the enzymolysis solution to separate the solid from the...

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PUM

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Abstract

The invention discloses a preparation method of pharmaceutical-grade sparassis crispa polysaccharide capable of enhancing immunity. The preparation method comprises the following steps of (1) pretreating raw materials: taking a proper amount of dried sparassis crispa sporocarps, and pulverizing the sparassis crispa sporocarps; and (2) taking sparassis crispa crude polysaccharide, adding an ionic liquid solution, performing stirring, adding enzyme for hydrolysis, filtering a hydrolysate, adding ethanol into a filtrate, performing standing, separating precipitate, and performing drying to obtain water-soluble sparassis crispa polysaccharide. According to the method, cellulose, fat and protein are removed from the sparassis crispa sporocarps by adopting an ionic liquid / compound enzyme system efficient specific enzymolysis extraction process, so that the yield of the sparassis crispa polysaccharide is increased. By adopting a green and environment-friendly ionic liquid / combined enzyme system directional hydrolysis technology, the viscosity of the sparassis crispa polysaccharide is reduced, the water solubility is increased, the absorption by the human body is better facilitated, and the effects of enhancing immunity, resisting tumors, reducing blood sugar and the like are remarkable.

Description

technical field [0001] The invention relates to the technical field of food, in particular to a preparation method and application of a pharmaceutical-grade hydrangea polysaccharide for enhancing immunity. Background technique [0002] Hydrangea, belonging to the order of Non-Pleatophores, Hydrangeaceae, Hydrangea genus. Hydrangea is rich in protein, amino acids, mineral elements, etc. It is not only delicious and nutritious, but also rich in active ingredients that are beneficial to the human body. It has the reputation of "King of Mushrooms" and "Sunshine Mushroom". The content of polysaccharide in hydrangea dry product is as high as 40-60%, which is an important active ingredient of hydrangea. Hydrangea polysaccharide is a biologically active substance. It has been confirmed by medical research that it has immune regulation, anti-tumor, anti-inflammatory, anti-virus, anti- Oxidation, anti-radiation, hypoglycemic, hypolipidemic, liver protection and other functions. Extr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/02A23L33/125A23L33/19A23L33/185A23L33/115A23L33/10A23L33/15A23L33/135A23L33/16C12R1/645
CPCC12P19/04C08B37/0024C08B37/0003A23L33/125A23L33/19A23L33/185A23L33/115A23L33/10A23L33/15A23L33/135A23L33/16A23V2002/00A23V2200/30A23V2200/324A23V2250/51A23V2250/54252A23V2250/54246A23V2250/5488A23V2250/5062A23V2250/28A23V2250/70
Inventor 黄芬邱英婷田健诸辉
Owner NINGBO XINUOYA MARINE BIOTECH CO LTD
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