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Corn stalk pre-processing method

A corn stalk and pretreatment technology, applied in the field of chemical engineering, can solve the problems of high cost and large amount of NMMO, and achieve the effect of reducing the amount of use, efficient enzymatic hydrolysis, and reducing the cost of pretreatment

Inactive Publication Date: 2016-10-05
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of pretreatment method of corn stalks, to solve the problem that the pretreatment method of the prior art uses too much NMMO and the cost is high

Method used

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  • Corn stalk pre-processing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 70% (w / w) NMMO, 15% (w / w) ethylene glycol and 15% (w / w) water mixture 10mL, add 0.1g dry corn stalks, and stir in an oil bath at 100°C for 2h after pretreatment, Add deionized water to regenerate. Electron microscope analysis and comparison of pretreated corn stalks and untreated corn stalks, as shown in Figure 1a with Figure 1b It was shown that the surface of pretreated corn stover was rough, and the dense advanced structure was destroyed and became loose, which fully exposed the cellulose, increased the accessibility of cellulase, and thus increased the saccharification rate.

Embodiment 2

[0041]75% (w / w) NMMO, 10% (w / w) ethylene glycol and 15% (w / w) water mixture 10mL, add 0.1g dry corn stover, after pretreatment at 130℃ for 0.5h, add Deionized water yields regenerated cellulosic material. After the regenerated cellulose material was washed 3 times with deionized water, a pH=4.8 acetic acid-sodium acetate buffer was added to form a mixed system, so that the final mass concentration of corn stalks was 10 g / L, and then cellulase (20 FPU / g corn stalks ) to carry out enzymolysis, shake the reaction for 96h on a constant temperature shaker at 50°C and 180rpm, the yield of reducing sugar is 65.4%, and the yield of glucose is 35.0% (such as figure 2 , image 3 ).

Embodiment 3

[0043] 80% (w / w) NMMO, 5% (w / w) ethylene glycol, 15% (w / w) water mixture 10mL, add 0.5g dry corn stalks, pretreat corn stalks at 130℃ for 1h , adding deionized water to obtain a regenerated cellulose material. After the regenerated cellulose material was washed 3 times with deionized water, a pH=4.8 acetic acid-sodium acetate buffer was added to form a mixed system, so that the final mass concentration of corn stalks was 10 g / L, and then cellulase (20 FPU / g corn stalks ) for enzymolysis, shaking and reacting on a constant temperature shaker at 50° C. and 180 rpm for 96 hours, the yield of reducing sugar was 64.7%, and the yield of glucose was 34.6%.

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Abstract

The invention discloses a corn stalk pre-processing method, which is used for pre-processing corn stalks by virtue of ethylene glycol assisted NMMO (N-methyl-N-morpholine oxide), wherein the method comprises the following steps: 1, pre-processing a corn stalk raw material by virtue of an NMMO-ethylene glycol-water mixed system, so that a regenerated cellulose material is obtained, wherein the mass of the NMMO is 70-80% of the NMMO-ethylene glycol-water mixed system and the mass of ethylene glycol is 5-15% of the NMMO-ethylene glycol-water mixed system; and 2, washing the generated cellulose material by virtue of de-ionized water, adding an acetic acid-sodium acetate buffer solution, so that a mixed system is formed, and then conducting enzymatic hydrolysis on a constant-temperature shaker with the addition of cellulase. The corn stalk pre-processing method provided by the invention can achieve efficient enzymatic hydrolysis of the corn stalks and reduce an NMMO dosage, so that processing cost is reduced.

Description

technical field [0001] The invention belongs to the field of chemical engineering and relates to a method for pretreating corn stalks with ethylene glycol-assisted N-methyl-N-morpholine oxide (NMMO). Background technique [0002] Biomass energy is a clean, low-emission, and cheap renewable energy. The production of fuel ethanol from biomass energy is an important part. my country is a large agricultural country, with about 700 million tons of crop straw produced every year, and rich lignocellulose resources. [0003] Lignocellulosic raw materials are mainly composed of cellulose, hemicellulose and lignin, and form a complex and dense structure, which reduces the direct contact of cellulosic enzymes and increases the difficulty of its efficient conversion into fermentable sugars. Therefore, it is necessary to pretreat lignocellulose to make crystalline cellulose become amorphous cellulose, destroy hydrogen bonds and some β-1,4 glycosidic bonds, reduce crystallinity and poly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 徐友海张跃张德胜何玉财朱庆伟严生虎谭春伟卿青
Owner PETROCHINA CO LTD
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