90results about How to "Achieve specific identification" patented technology

The invention discloses a rare earth organic framework material for iron ion fluorescence detection and a preparation method thereof. Methoxyl functional group-containing linear dicarboxylic acid (L-OCH3) is used as an organic raw material, a hydrothermal reaction is performed on the organic raw material and metal rare earth salt, and in the reaction process, an in-situ reaction is performed on methoxyl functional groups of an organic ligand to produce hydroxyl functional groups and the rare earth organic framework material is obtained. After adoption of the hydrothermal preparation method, the method is simple and the yield is high; the prepared rare earth organic framework material can be used as a fluorescent probe and interacts with iron ions, so that fluorescence of the organic ligandis quenched and new fluorescence generating a Ln2 (L-OH) 3-Fe<3+> compound is enhanced, and the intensity ratio between two peaks has a relatively good linear relationship with the concentration of the iron ions; the rare earth organic framework material can be applied to ratio detection of the iron ions.

The invention provides a micro-fluid control electrochemical biological sensing system for simultaneous detection on different serum markers of prostate cancer. The system comprises a continuous feeding unit, a micro-fluid control chip and a power system, wherein the continuous feeding unit is used for sequentially conveying a sample solution, a sample eluant, a signal probe solution, a signal probe eluant and an electrochemical detection buffer solution; the sample solution contains a protein marker and / or a miRNA marker for the prostate cancer; the micro-fluid control chip consists of one or more micro-channel networks; the micro-fluid control chip covers an electrode array so as to form a channel system; antibodies and / or capture probes which have interaction with the sample solution are fixed on the upper surface of the electrode array; the channel system is connected with the continuous feeding unit; the power system is used for providing power for the continuous feeding unit. The invention innovatively provides the micro-fluid control electrochemical biological sensing system which is capable of simultaneously detecting different serum markers closely related to prostate cancer diseases and is high in sensitivity and low in cost.

The invention relates to a spirit identifying system based on volatizing control and a visualized array sensor. The system comprises a volatizing device, a detecting device, a visualized array sensor and an image collecting instrument, wherein the volatizing device consists of an inner layer I and an outer layer I; the detecting device consists of an inner layer II and an outer layer II; the inner layer I is a spirit storing cavity; a heating cavity I is arranged between the inner layer I and the outer layer I; the inner layer II is a spirit gas detecting cavity; a heating cavity II is arranged between the inner layer II and the outer layer II; the visualized array sensor is arranged in the spirit gas detecting cavity; and the image collecting instrument is arranged at the top of the spirit gas detecting cavity. The spirit is volatized by the volatizing control device under the required condition and comes into contact with the visualized array sensor in the input detecting device, the signal responded by the feature of the sample to be detected is detected by the visualized array sensor, the spectrum change signal collected by the image collecting instrument is input to a signal identifying and processing system, the detecting results are displayed in a spectrogram way and then the visualized detection is realized. The detection is convenient and rapid by adopting the system.

The present invention discloses a preparation method and application of a printing electrode electrochemical immunosensor for simultaneous detection of multiple aflatoxins, and belongs to technical fields of biological sensing, nano-functional materials and food safety analysis. The surface of a rectangular substrate coated with a polyester film is printed with seven electrodes, including five carbon working electrodes, a silver reference electrode and a counter electrode, and with seven electrode lead lines and electrode lead line ends. The present invention is characterized in that: a silver nanoparticles-nitrogen-doped graphene-hydroxypropyl chitosan conductive composite nanomaterial is used for fixing aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, and total aflatoxin antibody to the surfaces of each working electrode, a detection liquid is dripped and coated on the working electrodes and the working electrodes are connected with a detection circuit of a multi-channel electrochemical workstation, thus the simultaneous detection of five aflatoxins are achieved. The sensor is simple in preparation, easy for processing, low in cost, and easy to carry. The detection method is simple, rapid, high in sensitivity and good in specificity.

The invention discloses an agricultural product pesticide residue detection method. The method comprises the following steps that template molecules, chitosan, hydroxyethyl acrylate, polyethyleneimineand deionized water are taken, imprinted hydrogel with the template molecules is synthesized; pretreatment of to-be-detected vegetables is completed, and a to-be-detected sample solution is prepared;the template molecules of the obtained imprinted hydrogel are removed; a standard Raman spectrogram of the template molecules is made; the imprinted hydrogel obtained after the template molecules areremoved is soaked in the to-be-detected sample solution and fished out, after confocal micro-Raman Spectrum detection is conducted, the obtained Raman Spectrum is compared with the standard Raman spectrogram, and then whether the template molecules are contained in samples or not is judged, and the content range of the molecules is judged preliminarily. Accordingly, the hydrogel with the specificrecognition function is prepared through a molecular imprinting technology, after the to-be-tested samples are adsorbed and enriched by the hydrogel, spectra collection is conducted by means of a portable Raman spectroscope, and whether the samples contain pesticide or not is judged by comparing with the standard Raman spectrogram.

The invention discloses a phenylethanolamine A molecularly imprinted membrane and a preparation method thereof. The method comprises steps of performing prepolymerization after phenylethanolamine A and methacrylic acid are mixed; adding ethylene glycol dimethacrylate and 2,2'-azodiisobutyronitrile into the system after prepolymerization to obtain a polymerization system; dipping a PVDF membrane in the polymerization system; subjecting the dipped PVDF membrane to a polymerization reaction in a nitrogen environment; and washing the PVDF membrane with methanol after the polymerization reaction is finished. Beneficial effects of the phenylethanolamine A molecularly imprinted membrane and the preparation method are that: the phenylethanolamine A molecularly imprinted membrane has advantages both of molecular imprinting technology and membrane separation technology; and the advantages comprise that (1) specific space sites and functional groups in the phenylethanolamine A molecularly imprinted membrane can achieve specific recognition of an object to be detected, and (2) compared with traditional molecularly imprinted microsphere materials, the molecularly imprinted membrane material provided by the invention is more stable, and more ordered in form, and can be used directly without tedious preparation processes such as a column packing process, etc..

The invention relates to an electrochemical aptamer sensor for detecting atrazine, and preparation and detection methods for the sensor, and overcomes the defects that an existing atrazine detection method is complex and high in cost, needs professional operators and the like. According to the electrochemical aptamer sensor, reduced graphene oxide is modified on the surface of a glassy carbon electrode to serve as a substrate electrode material, and nickel-iron cyanide complex nanoparticles are deposited on the surface of the electrode through an electrochemical deposition technology to serveas a signal probe; and gold nanoparticles are deposited on the surface of the electrode through electrochemical deposition, and an atrazine aptamer is fixed on the surface of the electrode through anAu-S key. The atrazine can be subjected to high-sensitivity analysis, and high selectivity can be obtained in a complex environment medium; the sensor has the advantages of being simple in instrumentequipment, low in analysis cost, fast in response and the like; and a new detection method can be provided for evaluating the residual components of the atrazine as a pesticide in the environment.

The invention discloses a method for rapidly assaying tumor-associated small peptide MUC1, which relates to the technical field of fluorescent probe assay. By utilizing the characteristic of specific recognition between the aptamer and the target small peptide, a special aptamer sequence is designed, and thereby the purpose of specific recognition of target objects is achieved; a signal amplification strategy is carried out in combination with the hybridization reaction of two types of hairpin probes, and thereby the sensitive assay of the target small peptide is realized. The method includes four steps, i.e. probe pre-denaturation, mixing reaction, background quenching and fluorescent assay, operation is easy and fast, time and labor can be saved, and a novel method is provided for the assay of tumor-associated small peptide MUC1.

The invention discloses a multi-channel electrode sensor for detecting ovarian cancer markers. The multi-channel electrode sensor comprises a substrate, an electrolytic tank region, four working electrodes, a reference electrode and an auxiliary electrode, wherein the electrolytic tank region is arranged on the substrate, and the working electrodes, the reference electrode and the auxiliary electrode are respectively arranged in the electrolytic tank region and are respectively connected with a multi-channel electrochemical working station detection circuit through mutually isolated electrode outgoing lines. The multi-channel electrode sensor for detecting the ovarian cancer markers has the advantages of convenience in manufacture, low cost, convenience in carrying, and easiness in minimization and integration, can simultaneously detect of four markers, is more convenient and rapid, is good in synchronization, is capable of reducing measurement errors, is remarkably increased in detection efficiency, and has important guiding significance for diagnosing, classifying and prognosing the tumor.

The invention relates to a preparation and application of a sulfo-carbamate hypochlorous acid fluorescent probe. Specifically, the probe of the invention is a sulfo-carbamate compound which can be used as a hypochlorous acid fluorescent probe for detecting hypochlorous acid. The probe can realize at least one of the following technical effects: the hypochlorous acid can be sensitively and high selectively identified and can be accurately detected; rapid response to the hypochlorous acid can be realized; instant detection of the hypochlorous acid can be realized; the properties are stable, and the probe can be stored and used for a long time; and the hypochlorous acid (in vivo / vitro) in a real sample can be detected.

The invention relates to a probe for ultra-sensitively and high-selectively detecting hypochlorous acid. Specifically, the probe provided by the invention is a coumarins compound, which can be used asa hypochlorous acid fluorescent probe for the detection of hypochlorous acid. The probe can achieve at least one of the following technical effects of ultra-sensitively and high-selectively identifying hypochlorous acid and performing accurate detection; rapidly responding to hypochlorous acid; realizing immediate detection on hypochlorous acid; being stable in property and being stored for use for a long time; being beneficial to detecting (in vivo / in vitro) hypochlorous acid in a practical sample.

The invention provides a method for initiating rolling circle amplification and FRET to detect miRNA based on a Toehold-mediated strand displacement reaction. The method comprises the steps that a rolling circle amplification reaction of the to-be-detected miRNA is initiated by utilizing the toehold-mediated strand displacement reaction, and the detection of the fluorescence resonance energy transfer is achieved by utilizing a DNA single strand, a DNA single-strand probe containing donor fluorophores and a DNA single-strand probe containing receptor fluorophores of the rolling circle amplification reaction. According to the method, by adopting the DNA single strand generated by combining the TMSD and the rolling circle amplification, a large quantity of fluorophores can be gathered, so that the gradual increasing tendency is generated instead of weakening the fluorescence intensity of the donor fluorophores, and therefore the effect of analyzing the content of the to-be-detected targetmiRNA is improved.

The invention relates to fluorescent probes represented by a general formula I or II which is described in the specification, and application of the fluorescent probes in detection of neurotoxic agents. The fluorescent probes can be used for efficiently detecting neurotoxic agents including of sarin, soman, tabun, ethyl sarin and the like of the G series and VX, VG, VM, VE and the like of the V series on the basis of a hybrid local area-charge transfer excited state and a dehybridization mechanism, and has the advantages of excellent detection limit, high sensitivity, good selectivity and recyclability.

The invention relates to a preparation and application of an ultra-sensitive high-selectivity hypochlorous acid fluorescence probe. Specifically, the probe is one category of fluorescein compound andcan be used as the hypochlorous acid fluorescence probe for detecting hypochlorous acid. The category of probes can realize at least one of the following technical effects: the hypochlorous acid can be identified in an ultra-sensitive high-selectivity way and can be accurately detected; the hypochlorous acid can be quickly responded; the hypochlorous acid can be detected immediately; properties are stable, and the ultra-sensitive high-selectivity hypochlorous acid fluorescence probe can be stored and used for a long time; the ultra-sensitive high-selectivity hypochlorous acid fluorescence probe is favorable for detecting (in vivo / in vitro) hypochlorous acid in a practical sample.

The invention relates to an ultra-sensitive high-selectivity hypochlorous acid colorimetric fluorescence probe. Concretely, the probe provided by the invention is a class of rhodamine compounds, and can be used as the hypochlorous acid colorimetric fluorescence probe for detecting hypochlorous acid. The class ofSuch probe can realize at least one of the following technical effects: the hypochlorous acid can be recognized in an ultra-sensitive high-selectivity way and is detected accurately; the hypochlorous acid can be quickly responded; the hypochlorous acid can be detected instantly; the probe is stable in property, can be stored and used for a long time, and is beneficial for detecting (in vivo / in vitro) hypochlorous acid in an actual sample.

The invention discloses a rare earth organic framework material for detecting vitamin C and a preparation method of the rare earth organic framework material. The rare earth organic framework material is of a long-range ordered crystal structure and is provided with regular pores, and the chemical formula of the rare earth organic framework material is [CeLx(G)(H2O)].(G)n, wherein L is an organic ligand simultaneously containing a tridiphenyl carboxylic acid group, G represents a solvent molecule coordinated with rare earth ions or in a crystal pore, x is equal to 1 to 3, and n is equal to 1 to 4. The rare earth organic framework material is prepared through a solvothermal method; the method is simple and relatively high in yield. The prepared rare earth organic framework material has a good detection effect on the vitamin C; with increase of the amount of the vitamin C, the luminous intensity of the rare earth organic framework material is improved, and the biocompatibility and the thermal stability are high. The rare earth organic framework material is expected to be applied to aspects such as biomedical treatment.

The invention relates to a peroxynitrite fluorescent probe, a preparation method and application thereof. The structural formula of the probe is shown as the specification. The probe can recognize ONOO<-> with high sensitivity and high selectivity and perform accurate detection. The probe has very weak fluorescence, when the probe is combined with ONOO<->, a compound A with strong fluorescence isobtained, the property is stable, and detection of ONOO<-> (in vivo / in vitro) in an actual sample is facilitated.

The invention provides a method for detecting hexavalent chromium ions based on a fluorescence ratio, and particularly relates to a method for detecting hexavalent chromium ions (Cr(VI)) based on a rhodamine derivative (Rhodol hydrazide) ratio. The specific detection method is as follows: the Rhodol hydrazide is taken as a fluorescent reagent, and the fluorescence emission intensity ratios of twodifferent wavelengths (577 nm and 502 nm) are measured in a 10mM sulfuric acid solution to be taken as a quantitative basis, thereby realizing ratio detection of the hexavalent chromium ions. The detection method shows high selectively and sensitivity to the hexavalent chromium ions, the detection process is simple and convenient, the interference resistance is strong, the detection is fast and sensitive, and the detection result is accurate.

The invention discloses a metal-organic framework material for detecting tryptophan. The material has a long-range ordered crystal structure and regular porous channels, and the chemical formula of the material is [ZnLx (H2O)m], wherein L is an organic ligand containing a benzenecarboxylic acid group and a pyridyl group, x is 0.5 to 1, and m is 1 to 1.5. The material is prepared by a solvothermal technology, and the synthesis method has the advantages of simplicity, easiness in obtaining of raw materials, and high yield. The prepared material has a good water stability and has a good tryptophan detection performance. The luminescence intensity of the prepared material gradually increases with the increase of the tryptophan concentration, and is not interfered by other amino acids. The material enables highly-efficiently and specifically identify the tryptophan, is expected to be applied to the field of biomedicines and the like.

The invention relates to a preparation method of cystamine modified charcoal for adsorbing heavy metal cadmium ions. The preparation method comprises the following steps of: S(a), activating charcoal; S(b), modifying the activated charcoal by cystamine. According to the preparation method disclosed by the invention, the charcoal is used as a carrier, glutaraldehyde is used as a cross-linking agent, the cystamine is modified to the surface of the charcoal so as to introduce a great quantity of active groups (sulfydryl, aldehyde, amino and imido group) to the surface of the charcoal, and the active groups can improve adsorption of the charcoal for heavy metals and particularly cadmium ions by a coordination effect and an electrostatic effect.

The invention relates to preparation and applications of a phenylboronic acid type hypochloric acid colorimetric fluorescence probe, and more specifically discloses a phenylboronic acid compound. The phenylboronic acid compound can be taken as a hypochloric acid colorimetric fluorescence probe used in hypochloric acid detection. The hypochloric acid colorimetric fluorescence probe is capable of achieving at least one of the following technical effects: high sensitivity selective identification of hypochloric acid and accurate detection; rapid response on hypochloric acid; and real-time detection of hypochloric acid. The phenylboronic acid type hypochloric acid colorimetric fluorescence probe is stable in properties, can be stored for a long term, and is beneficial for detection of hypochloric acid (in vivo / in vitro) in practical samples.

The invention discloses a preparation method and application of an electrochemical sensor for detecting transgenic soybeans based on double-signal output of a gene editing technology. The preparation method is characterized by comprising the following steps: synthesizing a magnetic material Fe3O4@AuNPs; a step of synthesizing the signal unit Fe3O4@AuNPs&DNA-Fc / [Ru(bpy)2]<2+; uniformly mixing a Cas12a enzyme solution and a crRNA solution, incubating at room temperature, adding a t-DNA solution and a binding buffer solution, uniformly mixing, incubating at room temperature, adding a signal unit solution, sucking a magnetic material part by adopting a magnetic separation method after cutting is finished, and re-dispersing the magnetic material part into 20 [mu]L of water; and finally, dripping the magnetic material part on the surface of the treated magnetic glassy carbon electrode, and obtaining the electrochemical sensor, detecting transgenic soybeans through the rapid scanning voltammetry technology and the electrochemical luminescence method, and therefore, the electrochemical sensor has the advantages of being high in sensitivity and selectivity and easy and rapid to operate.

The invention relates to fluorescence probes used for real-time sensitive high-selectivity recognition of hypochlorous acid. The probes are a type of 4-amino-1,8-naphthalimide compounds, and the compounds can be adopted as hypochlorous acid fluorescence probes for hypochlorous acid detection. The probes can achieve at least one of: high-sensitivity high-selectivity recognition of hypochlorous acidand accurate detection; capability of rapid response to the hypochlorous acid; capability of real-time detection of hypochlorous acid; stable properties and capability of long-term storage and use; and capability of facilitating detection of (in-vivo / in-vitro) hypochlorous acid in actual samples.

The invention provides a lead-chelated immune compound, and a preparation method and an application of the lead-chelated immune compound. The lead-chelated immune compound is one of the following compounds: a compound formed from an immune compound combined with lead ions, or a compound formed from carrier protein combined with lead and an antibody specifically binding to the carrier protein, or a compound formed from immune globulin combined with lead ions and carrier protein. The lead-chelated immune compound is an immune compound with relative specificity. Whether the lead-chelated immune compound is contained in serum of people in an area and the content of the compound is detected, so that the lead pollution degree in the area is indirectly reflected.

The invention provides a protein quantitative labeling reagent as well as a preparation method and application thereof. According to the protein quantitative labeling reagent provided by the invention, the high reaction activity of a o-phthalaldehyde group and a derivative group thereof and amino is utilized; specific recognition on lysine can be realized; the characteristics of high reaction activity and no hydrolysis of lysine are utilized; the labeling efficiency can be effectively improved, the relative abundance of reporter ions is compared so as to apply the reporter ions to the quantitative analysis of a plurality of lysine-containing proteomes, and the protein quantitative labeling reagent is stable in aqueous solution, can be stored for a long time, and is a new generation of thestable, efficient and economic equivalent ex-situ labeling reagent.

The invention relates to a speedy high-sensitivity high-selectivity hypochlorite identification colorimetric fluorescent probe. The speedy high-sensitivity high-selectivity hypochlorite identification colorimetric fluorescent probe particularly is an amino xanthene compound and can be used as a hypochlorite colorimetric fluorescent probe for detecting hypochlorite. The speedy high-sensitivity high-selectivity hypochlorite identification colorimetric fluorescent probe has at least one of the technical advantages that the hypochlorite can be identified in a high-sensitivity and high-selectivity manner and can be accurately detected; the speedy high-sensitivity high-selectivity hypochlorite identification colorimetric fluorescent probe can quickly response to the hypochlorite; the hypochlorite can be detected in real time; the speedy high-sensitivity high-selectivity hypochlorite identification colorimetric fluorescent probe is stable in property and can be stored and used for a long term; the speedy high-sensitivity high-selectivity hypochlorite identification colorimetric fluorescent probe is favorable for detecting (in-vivo / in-vitro) hypochlorite in actual samples.

The invention belongs to the technical field of protein and function research thereof, and particularly relates to a protein chemical cross-linking agent, a preparation method and applications thereof, wherein the structure of the protein chemical cross-linking agent is represented by a general formula I. According to the invention, the protein chemical cross-linking agent has the advantages of rapid reaction and no hydrolysis, shows efficient cross-linking activity when being applied to a CXMS technology, and can realize the specific recognition on arginine and lysine; and the results of thecross-linking reaction of the protein chemical cross-linking agent, six mode proteins, ten protein mixtures, multi-subunit yeast protein complex, 70S ribosome and exosome prove that the cross-linkingagent is effectively combined with lysine, so that the problems that the existing cross-linking agents are not rich in type, and the existing succinimide ester cross-linking agents have a lot of problems such as easy hydrolysis, low selectivity, slow cross-linking reaction and the like in the actual process of the CXMS technology are solved.

The invention provides a trinuclear copper alkynyl group complex with the amino acid recognition function and a preparation method of the trinuclear copper alkynyl group complex. According to the trinuclear copper alkynyl group complex with the amino acid recognition function and the preparation method of the trinuclear copper alkynyl group complex, a trinuclear copper alkynyl complex [Cu3([mu]-dppm)3([mu]3-[eta]<1>-C===CC6H4-C(O)H-4)2]BF4 is synthesized. The complex has a three-wire excited state of charge transfer from alkynyl ligand to trinuclear copper, and the specific recognition of cysteine and homocysteine can be realized by a fluorescence spectrum.

The invention discloses a preparation method of a high-sensitivity photoelectrochemical sensor for detecting mucoprotein. By growing a composite nano material of titanium dioxide and sulfur indium zinc on the surface of the indium tin oxide conductive electrode modified with the gold nanoparticles, more hairpin DNA chains can be fixed, and detection sensitivity is improved; then, a multi-branch hybrid chain connected with an aptamer and modified with manganese dioxide particles is loaded on the surface of the electrode, has good catalytic reduction capability on hydrogen peroxide, and is usedas a mimic enzyme signal label to consume an electron donor hydrogen peroxide to realize amplification of an analysis signal; through recognition and enzyme digestion of exonuclease, signal amplification of mucoprotein is further realized, so preparation of the photoelectrochemical sensor is completed, and ultra-sensitive and accurate detection of mucoprotein is realized.

The invention discloses an agricultural product pesticide residue detection method. The method comprises the following steps that template molecules, chitosan, hydroxyethyl acrylate, polyethyleneimineand deionized water are taken, imprinted hydrogel with the template molecules is synthesized; pretreatment of to-be-detected vegetables is completed, and a to-be-detected sample solution is prepared;the template molecules of the obtained imprinted hydrogel are removed; a standard Raman spectrogram of the template molecules is made; the imprinted hydrogel obtained after the template molecules areremoved is soaked in the to-be-detected sample solution and fished out, after confocal micro-Raman Spectrum detection is conducted, the obtained Raman Spectrum is compared with the standard Raman spectrogram, and then whether the template molecules are contained in samples or not is judged, and the content range of the molecules is judged preliminarily. Accordingly, the hydrogel with the specificrecognition function is prepared through a molecular imprinting technology, after the to-be-tested samples are adsorbed and enriched by the hydrogel, spectra collection is conducted by means of a portable Raman spectroscope, and whether the samples contain pesticide or not is judged by comparing with the standard Raman spectrogram.