The invention relates to a method for efficiently separating and purifying multiresidue sulfonamide
antibiotics in a biological sample. The method is characterized by comprising the following steps of: (1) preparing a molecular imprinted
polymer, comprising the steps of burdening according to the mol ratio of template molecule to
functional monomer to cross linking agent of being 1: (2-8): 25 and obtaining the molecular imprinted
polymer with special identification function to the sulfonamide
antibiotics by adopting a body
polymerization method; (2) weighing certain amount of biological sample to be separated into a
mortar,
grinding the biological sample by the
mortar, adding right amount of the molecular imprinted
polymer, and further homogenating to obtain a homogenated sample, wherein the weight ratio of the biological sample to be separated to the molecular imprinted polymer is 2: (1-5), and (3) placing the homogenated sample in a
solid phase extraction column, sequentially rinsing with 2.0-4.0ml of water, 2.0-4.0ml of 10wt%
acetonitrile aqueous solution, and then eluting with 3.0-5.0ml of
methanol / methanoic acid
mixed solution; and separating the sulfonamide
antibiotics from the biological sample to be separated by being instantly dissolved in the
methanol / methanoic acid
mixed solution. The method provided by the invention has wide selectivity and good separation and purification effect.