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Electrochemical aptamer sensor for detecting atrazine, and preparation and detection methods for sensor

An aptamer sensor and atrazine technology, applied in the direction of electrochemical variables of materials, etc., can solve the problems of reduced analytical performance, cumbersome operation, poor selectivity, etc., and achieve simple equipment, low analysis cost, and high selectivity. Effect

Active Publication Date: 2019-01-15
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of high cost, cumbersome operation, and poor selectivity of the existing atrazine detection method, and at the same time overcome the problems that the previous electrochemical aptamer sensors need to label the aptamer or add electroactive substances to the test system. Due to the shortcomings of cumbersome operation and reduced analytical performance, a simple and rapid electrochemical aptasensor for the detection of atrazine and its preparation and detection methods are provided

Method used

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  • Electrochemical aptamer sensor for detecting atrazine, and preparation and detection methods for sensor
  • Electrochemical aptamer sensor for detecting atrazine, and preparation and detection methods for sensor
  • Electrochemical aptamer sensor for detecting atrazine, and preparation and detection methods for sensor

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Experimental program
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Effect test

Embodiment 1

[0036] This embodiment proposes a method for preparing an electrochemical aptasensor for detecting atrazine, comprising the following steps:

[0037] S101. Pretreatment of the glassy carbon electrode GCE.

[0038] Glassy carbon electrodes GCE were used with 1.0, 0.3, 0.05 µm Al 2 o 3 After powder polishing, ultrasonically clean in ethanol and high-purity water for 1 to 5 minutes, and finally in N 2 The atmosphere is dry.

[0039] S102, preparation of ERGO / GCE electrodes.

[0040] Place the treated glassy carbon electrode GCE in a pure aminopropyl triethoxy silicon solvent with a purity of 98% and immerse it for 20-30 minutes, aminate the surface of the glassy carbon electrode GCE, then rinse it with secondary water, and Dry in N2 atmosphere until the surface of the electrode is free of water beads and becomes a smooth mirror surface.

[0041] Then, 1 mg / L graphene oxide GO solution was drop-coated on the surface of the surface-aminated glassy carbon electrode GCE, and dri...

Embodiment 2

[0051] This embodiment provides a detection method for an electrochemical aptasensor for detecting atrazine, comprising the following steps:

[0052] S201, preparing a plurality of atrazine standard solutions with different concentrations;

[0053] S202. Using the electrochemical aptamer sensor for detecting atrazine as a working electrode, a saturated calomel electrode as a reference electrode, and a platinum sheet electrode as a counter electrode, in an electrolyte solution of 0.1 M PBS, pH7.4 Atrazine was determined by differential pulse voltammetry. Due to the specific recognition of the aptamer and its target substance, atrazine interacts with the nucleic acid aptamer modified on the electrode surface, and atrazine is captured on the electrode surface to form an aptamer-atrazine The complex prevents the electron transfer between the solution and the electrochemical sensing interface, resulting in a decrease in the redox peak current of the nickel-ferricyanide complex nan...

Embodiment 3

[0057] Using 0.1 M PBS solution (pH 7.4) as the electrolyte solution, the prepared electrochemical aptamer sensor was used as the working electrode, the saturated calomel electrode was used as the reference electrode, and the platinum electrode was used as the counter electrode. Somatosensor selectivity for atrazine. Prepare samples of the substance to be tested, atrazine, and interfering substances (the concentration of the interfering substances is 100 times that of atrazine), and the interfering substances are simazine, propanil, malathion, 2,4- D. A mixed solution of p-nitrophenol, 2,2-binaphthol, bisphenol A and all above-mentioned interfering substances and atrazine. Using the test conditions in Example 2, the above solutions were respectively added into the test system, and the differential pulse method was used for detection.

[0058] Such as Figure 5 As shown, first, the sensor prepared by the above method is used to detect atrazine (the first bar graph), and then ...

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Abstract

The invention relates to an electrochemical aptamer sensor for detecting atrazine, and preparation and detection methods for the sensor, and overcomes the defects that an existing atrazine detection method is complex and high in cost, needs professional operators and the like. According to the electrochemical aptamer sensor, reduced graphene oxide is modified on the surface of a glassy carbon electrode to serve as a substrate electrode material, and nickel-iron cyanide complex nanoparticles are deposited on the surface of the electrode through an electrochemical deposition technology to serveas a signal probe; and gold nanoparticles are deposited on the surface of the electrode through electrochemical deposition, and an atrazine aptamer is fixed on the surface of the electrode through anAu-S key. The atrazine can be subjected to high-sensitivity analysis, and high selectivity can be obtained in a complex environment medium; the sensor has the advantages of being simple in instrumentequipment, low in analysis cost, fast in response and the like; and a new detection method can be provided for evaluating the residual components of the atrazine as a pesticide in the environment.

Description

technical field [0001] The invention belongs to the field of electrochemical aptamer sensors, and relates to an electrochemical aptamer sensor for detecting atrazine and a preparation and detection method thereof. Background technique [0002] Atrazine, also known as atrazine, has a chemical name of 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine and is a triazine pesticide. Since it was put into commercial production, it has been widely promoted and used in the world. A large number of studies have shown that atrazine has a stable molecular structure and is not easy to degrade, and it is widely present in water and soil. Especially as an environmental hormone, it has serious harm to the growth and reproduction of aquatic organisms and may produce degenerative reactions; at the same time, it is also one of the potential carcinogens for humans. Therefore, it is of great significance to seek a rapid, reliable, highly sensitive and highly selective analytical method for...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 范丽芳张彩云郭玉晶梁文婷刘雪峰董川
Owner SHANXI UNIV
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