A kind of Acetobacter xylinum improved fermentation medium

A fermentation medium, the technology of Acetobacter xylinum, applied in the field of improved fermentation medium of Acetobacter xylinum, can solve the problems of prolonging the fermentation period, reducing the activity of Acetobacter xylinum, etc., so as to increase the final yield, shorten the fermentation period, and improve production efficiency. Improved effect

Active Publication Date: 2019-10-18
NANJING UNIV OF SCI & TECH
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  • Claims
  • Application Information

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Problems solved by technology

Document 3 (JUNG WOOK HWANG, et al..Effects of pH and Dissolved Oxygen on Cellulose Production by AcetobacterxyZh.m~BRCS in Agitated Culture, 1999, 88:183-188) reported that Acetobacter xylinum produced cellulose by fermentation, Gluconic acid will be produced, causing the pH to drop, thereby reducing the activity of Acetobacter xylinum, resulting in a longer fermentation cycle

Method used

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  • A kind of Acetobacter xylinum improved fermentation medium
  • A kind of Acetobacter xylinum improved fermentation medium
  • A kind of Acetobacter xylinum improved fermentation medium

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preparation example Construction

[0016] The preparation of seed liquid among the present invention and parameter monitoring can refer to existing method, and wherein seed liquid can be made through the following steps:

[0017] The strains preserved at 4°C were kept at 30°C for 20 minutes, and a ring of strains was picked up with an inoculation loop and streaked on the solid plate medium, and the plate was cultured in a 30°C incubator for 36 hours. Composition of solid medium (g / 100mL): glucose 2.0, sucrose 1.0, magnesium sulfate 0.04, citric acid 0.11, sodium dihydrogen phosphate 0.25, peptone 1.0, agar 1.8, yeast extract powder 0.1. pH=6.0. Sterilize at 121°C for 30 minutes;

[0018] Pick 2-3 loops of the obtained activated seeds with an inoculation loop, inoculate them into a 500mL Erlenmeyer flask containing 1 / 5 of the seed solution, and then put them in a shaker and shake them back and forth at 120-160rpm for 48h. Seed solution composition (g / 100mL): glucose 2.0, ammonium sulfate 0.6, potassium dihydrog...

Embodiment 1

[0023] The seed solution was added to 40 shake flasks, and each shake flask contained 100mL of fermentation broth, and the addition ratio was 5mL of seed solution to 100mL of fermentation broth. Fermentation broth composition (g / 100mL): glucose 2.5, sucrose 3.0, ammonium sulfate 0.12, potassium dihydrogen phosphate 0.6, magnesium sulfate 0.07, calcium lactate 0.03, peptone 1.5, yeast extract powder 1.0, acetic acid 0.3, citric acid 0.08, carboxymethyl Base cellulose sodium 0.04. Mark the shaker flasks into 1-4 groups, 10 in each group, and add gluconic acid / sodium gluconate (1:1): 0g, 2g, 5g, 10g to each group respectively. Sterilize at 121°C for 30 minutes before adding the seed solution.

[0024] The above 40 shake flasks were placed in a shaker at 160-200rpm for dynamic culture. Take out each shake flask in 1~4 groups every 8h, carry out the mensuration of cellulose content according to the method for BC production monitoring in parameter monitoring, the result is as foll...

Embodiment 2

[0027] In order to further obtain the yield change in the fermentation process, the sampling interval can be further shortened to 4h.

[0028] The seed solution was added to 40 fermentation broths containing 100mL, and the addition ratio was 8mL seed solution to 100mL fermentation broth. Fermentation broth composition (g / 100mL): glucose 2.25, sucrose 2.75, ammonium sulfate 0.1, potassium dihydrogen phosphate 0.5, magnesium sulfate 0.07, calcium lactate 0.02, peptone 1.0, yeast extract powder 0.75, acetic acid 0.15, citric acid 0.06, carboxymethyl Base cellulose sodium 0.04. To 20 of these shake flasks, 5 g of gluconic acid / sodium gluconate (1:3) was added to each shake flask, and the bottles containing gluconic acid / sodium gluconate were marked as category A1 and the rest as category B1. Sterilize at 121°C for 30 minutes before adding the seed solution.

[0029] The above 40 shake flasks were placed in a shaker at 160-200rpm for dynamic culture. Every 4h, take out each shak...

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Abstract

The invention discloses an acetobacter xylinum improved fermentation culture medium. 50-100 g / L of glucose acid / sodium gluconate is added in the acetobacter xylinum improved fermentation culture medium, the added glucose acid / sodium gluconate solution can form a buffer system, the pH in the bacterial cellulose fermentation production process is effectively controlled, and the pH is controlled in a range suitable for breeding and growth of bacteria. The addition of the fermentation intermediate glucose acid can make the acetobacter xylinum faster enter a fast synthesis stage, and the production efficiency is greatly improved. At the same time, the glucose acid / sodium glucose is used as an additional carbon source, and can effectively improve the final yield of the whole fermentation process based on shortening of the fermentation cycle.

Description

technical field [0001] The invention relates to an improved fermentation medium of Acetobacter xylinum, in particular to an improved fermentation culture of Acetobacter xylinum which can shorten the fermentation period of Acetobacter xylinum and increase cellulose production by adding gluconic acid / sodium gluconate as a buffer solution and a carbon source base, belonging to the field of biotechnology. Background technique [0002] In the process of artificially synthesizing cellulose, low yield and high energy consumption due to long fermentation cycle have always been difficult problems. The existing technology mainly shortens the fermentation time by controlling key culture conditions such as pH, temperature, and dissolved oxygen. and increase the final yield. [0003] In recent years, a variety of methods have been used to control the pH in the fermentation process. Most of them are directly connected to a certain amount of strong acid and strong alkali for local adjustm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/02
Inventor 孙东平顾焱黄洋陈春涛自强孙汴京梁光芸
Owner NANJING UNIV OF SCI & TECH
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