271 results about "Acetobacter xylinum" patented technology

The invention relates to a method for preparing a bacterial cellulose gel composite material, which comprises the following steps: preparing culture solution; inoculating a strain of acetobacter xylinum into the culture solution, carrying out static culture for 2 days at a temperature of 30 DEG C to generate a bacterial cellulose thin film, dispersing the bacterial cellulose thin film under the aseptic condition, and centrifuging after carrying out enzymolysis on the bacterial cellulose thin film so as to obtain bacterial inoculated solution; preparing bacteria solution from the bacterial inoculated solution and normal saline according to a volume ratio of 1:1 to 1:3; after preprocessing a composite ligand, sterilizing the composite ligand to obtain aseptic gel; sufficiently mixing the aseptic gel and the bacteria solution according to a volume ratio of 1:1 to 3:1, dropwise adding the mixture into first aseptic calcium chloride solution to form gel complex globule, then transferring the gel complex globule into second aseptic calcium chloride solution to carry out standing for 6 to 12 hours, placing the obtained product into newly prepared culture solution and carrying out rotating fermentation for 2 to 3 days so as to obtain a bacterial cellulose gel composite base material; and carrying out rotating fermentation or static fermentation on the bacterial cellulose gel composite base material for 2 to 5 days so as to obtain the ball-like or film-shaped bacterial cellulose gel composite material. The method is high in composite rate and in material utilization rate.

The invention discloses a vascular stent material of tissue engineering and manufacturing method thereof, which comprises selecting acetobacter xylinum as a model strain; using oxygen permeable materials to prepare hollow tubular dies which are arranged in the fermentation liquid and introduced with oxygen for fermentation until the bacterial celluloses completely cover the outer surface of the dies and the required porosity, thickness, uniformity and structural characteristic are reached to obtain a vascular stent raw material of tissue engineering; removing impurities to reduce the impurity rate to below 0.05%, sterilizing and packaging to prepare the vascular stent material of tissue engineering. The invention is simple in process and safe and has no toxic and side effect, and the material has more uniform spatial structure, more suitable porosity, uniform texture of each material in batches by controlling the fermentation process to effectively improve the quality and favourably achieve the scaled production.

The invention discloses a preparation method of a water-soluble carbon dot and application of the water-soluble carbon dot in microbial fermentation, belonging to the technical field of new energy and new materials. According to the preparation method, river crab shell waste is as the raw material, and the acquired carbon dot fluorescence quantum yield can achieve 35% through a simple thermal cracking process. The prepared water-soluble fluorescence carbon dot has biocompatibility, and has an obvious acceleration effect on the fermentation of pneumonia klebsiella pneumonia, the yield of 1,3-propylene glycol can be improved by 30%. Furthermore, the water-soluble fluorescence carbon dot is applied to the standing fermentation of the acetobacter xylinum, the product bacteria cellulose yield can be improved by 75%. The water-soluble fluorescence carbon dot is applied to the microbial fermentation for the first time, and an obvious promotion effect is achieved, and a new growth factor is provided for the microbial fermentation.