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Method for fermented-producing bacteria cellulose with pineapple peel juice by two-step method

A technology of bacterial cellulose and two-step method, which is applied in the fields of production and comprehensive utilization of pineapple peels, can solve the problems of cellulose produced by fermentation without separate production, restrictions on large-scale production, pollution of the ecological environment, etc., and achieve shortening of static culture time, shorten production time, and control the effect of contamination by miscellaneous bacteria

Inactive Publication Date: 2010-03-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are two main problems in the existing bacterial cellulose production technology: (1) the fermentation cycle is long, up to 7 to 12 days; (2) shallow plate fermentation is used, and the growth and reproduction of bacteria are not separated from the production of cellulose produced by fermentation. The fermentation cycle is long, the production efficiency is low, and large-scale production is limited
A large amount of pineapple peel and slag are produced during pineapple processing, which seriously pollutes the ecological environment

Method used

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  • Method for fermented-producing bacteria cellulose with pineapple peel juice by two-step method
  • Method for fermented-producing bacteria cellulose with pineapple peel juice by two-step method

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Embodiment 1

[0030] A kind of method that utilizes pineapple peel juice to carry out " two-step method " fermentation to produce bacterial cellulose, comprises the steps:

[0031] The first step of activating strains: Acetobacter xylinum ATCC 23767 (Gluconacetobacter xylinum, referred to as Acetobacter xylinum) is preserved at the China Ordinary Microbiology Collection and Management Center at a ratio of 0.01% by weight, address: Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, Zip code: 100101, Tel: 86-10-64807596) strains are inserted in the seed culture medium of 100ml, at 25 DEG C, cultivate 10h in 150rpm, make mother starter; the formula of described seed culture medium is: peptone 20g, 980g of coconut water, stirred and dissolved evenly, adjusted the pH to 4.5 with acetic acid, sterilized with 0.10MPa for 15min, cooled to 25°C for later use.

[0032] The second step is a large number of bacterial proliferation cultures: the mother starter is inserted into the working sta...

Embodiment 2

[0036] A kind of method that utilizes pineapple peel juice to carry out " two-step method " fermentation to produce bacterial cellulose, comprises the steps:

[0037] The first step of activating strains: Acetobacter xylinum ATCC 23767 (Gluconacetobacter xylinum, referred to as Acetobacter xylinum) in a ratio of 0.50% by weight, is preserved by China Ordinary Microorganisms Collection and Management Center, address: Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, Zip code: 100101, Tel: 86-10-64807596) the strains are inserted into 200ml of seed culture medium, cultivated at 22°C and 100rpm for 8h to make mother starter; by weight fraction, the seed culture medium formula For: peptone 5g, coconut water 995g, stir and dissolve evenly, adjust the pH to 5.5 with acetic acid, sterilize with 0.08MPa for 20min, cool to 22°C for later use.

[0038] The second step is a large number of bacterial proliferation cultures: with a volume ratio of 4:100, insert the mother sta...

Embodiment 3

[0042] A kind of method that utilizes pineapple peel juice to carry out " two-step method " fermentation to produce bacterial cellulose, comprises the steps:

[0043] The first step of activating strains: Acetobacter xylinum ATCC 23767 (Gluconacetobacter xylinum, referred to as Acetobacter xylinum) in a ratio of 0.25% by weight, is preserved by the China Ordinary Microorganisms Collection Management Center, address: Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, Zip code: 100101, Tel: 86-10-64807596) strains are inserted in 150ml seed culture medium, at 28 ℃, cultivate 12h in 200rpm, make mother starter; by weight fraction, described seed culture medium formula It is: 40g of peptone, 960g of coconut water, stir and dissolve evenly, adjust the pH to 5.0 with acetic acid, sterilize with 0.09MPa for 18min, cool to 28°C for later use. The second step is a large number of bacterial proliferation culture: the mother starter is inserted into the working starter cultu...

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Abstract

The invention discloses a method for fermented-producing bacteria cellulose with pineapple peel juice by a two-step method. In the method, two stages, including multiplication of acetobacter xylinum and fermented production of bacteria cellulose, are separated from each other, sterile air is ceaselessly fed in at 22 to 28 DEG C in the process of fermentation and acetobacter xylinum is cultivated for 24 to 72 hours under 100 to 200rpm stirring, at this moment, the viable count of acetobacter xylinum is 10<8> to 10<9>cfu / ml, cells are subject to multiplication culture of bacteria to be in a synchronous growth period as far as possible, and the cells are then subject to shallow tray static cultivation in a fermentation medium for fresh juice of the pineapple peel so as to result in bacteria cellulose with the yield more than 17.5g / L. The method greatly shortens the production period of bacteria cellulose, makes full use of the pineapple peel and protects environmental, and in addition, the produced bacteria cellulose is compact and uniform in texture.

Description

technical field [0001] The invention relates to the production of bacterial cellulose and the comprehensive utilization of pineapple peel, in particular to a method for producing bacterial cellulose by "two-step" fermentation using pineapple peel juice. Background technique [0002] Bacterial cellulose has good water permeability, air permeability, strong hydrophilicity, extraordinary water holding capacity (absorbing 600-700 times its dry weight of water) and high wet strength, so it has many potential applications. Bacterial cellulose has been commercially applied in audio, food, daily chemical, medicine, papermaking and other industries, and is considered to be the best cellulose in the world. Since the product was launched, it has been selling well in the international market. The production capacity of existing bacterial cellulose production enterprises is far from meeting the large demand for bacterial cellulose in the market. Therefore, improving the fermentation pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02
Inventor 刘冬梅吴晖于淑娟
Owner SOUTH CHINA UNIV OF TECH
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