Bacteria cellulose producing bacteria and method for preparing bacteria cellulose using above bacterial strain

A technology for bacterial cellulose and bacteria production, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high energy consumption, complicated process, low yield, etc., and achieve the effect of high yield

Active Publication Date: 2009-03-11
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dynamic fermentation method consumes relatively high energy and the process is more complicated, but it is more suitable for large-scale production, and the yield of bacterial cellul

Method used

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  • Bacteria cellulose producing bacteria and method for preparing bacteria cellulose using above bacterial strain
  • Bacteria cellulose producing bacteria and method for preparing bacteria cellulose using above bacterial strain

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Get the slant seed that preserves in 4 ℃ of refrigerators for 5 days [the composition of slant medium (g / L): glucose 80, yeast extract 8, calcium carbonate 15, agar 15, all the other components are water, pH6.5. Cultivate 24 at 28 ℃. hours], use an inoculation needle to inoculate the above slope about 1cm 2 The bacterial classification of area is scraped off, transfers in the liquid activation medium of new configuration [composition (g / L) of liquid activation medium: glucose 80, yeast extract 8, peptone 5, all the other components are water, adjust pH value to 6.0. ], the liquid activation medium was packed in a 250ml Erlenmeyer flask, and the amount of the Erlenmeyer flask was 20ml, and it was shaken and cultivated in a reciprocating shaker at 28°C for 18 hours, the shaker speed was 80r / m, and the stroke was 65mm. Take the above-mentioned cultured liquid activation medium and inoculate it into a new configuration, sterilized at 121°C for 15 minutes and cooled to room...

Embodiment 2

[0029] Get the slant seed that preserves in 4 ℃ of refrigerators for 5 days [the composition of slant medium (g / L): glucose 80, yeast extract 8, calcium carbonate 15, agar 15, all the other components are water, pH6.5. Cultivate 24 at 28 ℃. hours], use an inoculation needle to inoculate the above slope about 1cm 2 The bacterial classification of area is scraped off, transfers in the liquid activation medium of new configuration [composition (g / L) of liquid activation medium: glucose 80, yeast extract 8, peptone 5, all the other components are water, adjust pH value to 6.0. ], the liquid activation medium was packed in a 250ml Erlenmeyer flask, and the amount of the Erlenmeyer flask was 20ml, and it was shaken and cultivated in a reciprocating shaker at 28°C for 18 hours, the shaker speed was 100r / m, and the stroke was 75mm. Take the above-mentioned cultured liquid activation medium and inoculate it into a new configuration, sterilized at 121°C for 15 minutes and cooled to roo...

Embodiment 3

[0031] Get the slant species preserved in the refrigerator for 3 days at 4°C, [the composition of the slant medium (g / L): glucose 100, yeast extract 10, calcium carbonate 25, agar 18, and the remaining components are water, pH7.2. Cultivate at 32°C 36 hours], use an inoculation needle to inoculate about 1cm on the above slope 2 The bacterial classification of area is scraped off, transfers in the liquid activation medium of new configuration [composition (g / L) of liquid activation medium: glucose 100, yeast extract 10, peptone 7.5, all the other components are water, adjust pH value to 7.0. ], the liquid activation medium was packed in a 250ml Erlenmeyer flask, and the amount of the Erlenmeyer flask was 20ml. It was shaken and cultivated in a reciprocating shaker at 32°C for 28 hours. The shaker speed was 90r / m, and the stroke was 75mm. Get the above-mentioned cultivated liquid activation medium and inoculate it into the fermentation medium newly configured, sterilized at 121...

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Abstract

The invention provides bacterial cellulose (BC) producing bacteria and a method for preparing the bacterial cellulose by using the bacterial strain. The bacterial strain is a selected gluconic acid acetobacter xylinum GD-BC-1 with an accession number of CCTCC M 208149. The method is to use the bacterial strain as a fermentation bacterial strain and use glucose as a main fermentation material for fermentation so as to obtain the BC. The preparation method comprises the following steps of: activating a slant bacterial strain, liquid-activating the slant bacterial strain, inoculating the liquid-activated slant bacterial strain in a fermentation culture medium to perform dynamic or static fermentation and extracting products of BC from the fermentation solution. The preparation method uses the bacterial strain of gluconic acid acetobacter xylinum GD-BC-1 with the accession number of CCTCC M 208149 for fermentation so as to produce the BC. The BC can be produced through dynamic or static fermentation in a conical flask with glucose as the main fermentation material, and the yield is high and different methods can be used in different production environment to produce BC.

Description

technical field [0001] The invention relates to a microbial strain for producing bacterial cellulose and a method for fermenting and producing bacterial cellulose by using the bacterial strain. Background technique [0002] Cellulose is the most abundant biopolymer on the earth, and it is the main component of plant biomass. The cellulose produced by plants on the earth reaches hundreds of millions of tons every year. In addition to plants, some lower animals can produce animal cellulose (Tunlcin), and some bacteria can produce extracellular bacterial cellulose (Bacterial cellulose, hereinafter referred to as BC) in a heterotrophic manner more efficiently and with higher purity than plants. In 1886, A.J.Brown firstly reported that Acetobacter xylinum could synthesize an extracellular gel-like substance (A.J.Brown.On an aceticferment forms cellulose[J].J.Chem.Soc, 1886, 49 : 432-439.), but due to the lack of suitable experimental methods and the low yield of cellulose cords,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C12R1/01
Inventor 施庆珊欧阳友生陈仪本冯静疏秀林陈爱美谢小保冯劲
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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