Preparation method of biology cellulose composite membrane face-mask material
A bio-cellulose, composite membrane technology, applied in pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc., can solve the problems of mask performance impact, poor water-holding performance, inconvenient use, etc., to achieve strong moisturizing, high yield The effect of improving and high utilization rate of raw materials
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Embodiment 1
[0037] (1) Selection of strains: select Gluconacetobacter xylinum CGMCC No.1.1812;
[0038] (2) Preparation of carboxymethyl cellulose seeds and liquid culture medium: In terms of weight percentage, the formula is composed as follows:
[0039] A: Glucose 2%
[0041] C: Tryptone 0.5%
[0042] D: Na 2 HPO 4 0.5%
[0043] E: Citric acid 0.2%
[0044] F: calcium carbonate 2%
[0045] The balance is water;
[0046] Add 0.2g / L carboxymethyl cellulose to the seed medium or liquid medium, then adjust the pH of the medium to 5, sterilize at 121°C for 20 minutes, and cool to 30°C for later use;
[0047] (3) Cultivation of seed cells: Take 1-2 loops of activated slant seeds and insert them into a liquid culture medium containing carboxymethyl cellulose under aseptic operating conditions, at 28°C on a shaker with a rotation speed of 140r / min Shake culture for 24 hours to obtain seed culture solution;
[0048] (4) Production of bio-cellulose composit...
Embodiment 2
[0051] (1) Selection of strains: select Gluconacetobacter xylinum CGMCC No. 1.2378;
[0052] (2) Preparation of carboxymethyl cellulose seeds and liquid culture medium: In terms of weight percentage, the formula is composed as follows:
[0053] A: Fructose 4%
[0055] C: Tryptone 1%
[0056] D: Na 2 HPO4 1%
[0057] E: Citric acid 0.3%
[0058] F: calcium carbonate 3%
[0059] The balance is water;
[0060] Add 0.8g / L of carboxymethylcellulose to the seed medium or liquid medium, then adjust the pH of the medium to 6, sterilize at 121°C for 25 minutes, and cool to 30°C for later use;
[0061] (3) Cultivation of seed cells: Take 1-2 loops of activated slant seeds and put them in a liquid culture medium containing carboxymethyl cellulose under aseptic operation conditions at 32°C on a shaker with a rotation speed of 180r / min Shake culture for 36 hours to obtain seed culture solution;
[0062] (4) Static liquid fermentation to produce bio-cellu...
Embodiment 3
[0065] (1) Selection of strain: select Gluconacetobacter xylinum CGMCC No. 1.1812;
[0066] (2) Preparation of carboxymethyl cellulose seeds and liquid culture medium: In terms of weight percentage, the formula is composed as follows:
[0067] A: Sucrose 3%
[0068] B: Yeast powder 0.8%
[0069] C: Tryptone 0.8%
[0070] D: Na 2 HPO 4 0.8%
[0071] E: Citric acid 0.25%
[0072] F: calcium carbonate 2.5%
[0073] The balance is water;
[0074] Add 0.5g / L of carboxymethylcellulose to the seed medium or liquid medium, then adjust the pH of the medium to 6, sterilize at 121°C for 20 minutes, and cool to 30°C for later use;
[0075] (3) Cultivation of seed cells: Take 1-2 loops of activated slant seeds, insert them into a liquid culture medium containing carboxymethyl cellulose under aseptic operation conditions, and place them on a shaker with a rotation speed of 160r / min at 30°C. Shake culture for 30 hours to obtain seed culture solution;
[0076] (4) Production of bio-ce...
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