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New method for producing bacterial cellulose

A technology for bacterial cellulose and seeds, applied in the field of fermentation engineering, can solve the problems of inability to produce bacterial cellulose on a large scale, increase the shear force of the bacteria, and reduce the shear force, so as to achieve a small footprint, improve yield and production. Efficiency, the effect of short production cycle

Active Publication Date: 2012-02-01
贵州国芯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a new method for producing bacterial cellulose, to solve the problem that the growth of bacteria in shallow plate static fermentation method is inhibited due to insufficient oxygen supply, and to solve the problem that traditional dynamic fermentation increases the efficiency of oxygen transfer while increasing For the shear force of the bacteria, some shear force-sensitive strains cannot be used for the large-scale production of bacterial cellulose due to the physiological requirements of low shear force

Method used

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  • New method for producing bacterial cellulose
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  • New method for producing bacterial cellulose

Examples

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Embodiment 1

[0027] Example 1: After mixing the saccharified liquid with a total sugar content of 171.28g / L and the alcoholic fermented mash with an ethanol content of 8.24% v / v at a ratio of 3:5 (v:v), accurately measure 8L of the above mixed liquid and add it to boiling water Sterilize the bottom of the pouring fermentation tower for 30 minutes, transfer 7.5 kg of corncobs and 5 kg of rice husks sterilized at 121°C to the packing layer above the false bottom of the pouring fermentation tower while hot (the corncobs are stacked naturally, and the rice hulls are placed on the corn husks). The bottom and top of the core are laid flat in two layers), and after the top cover is covered, the jacket is forced to cool by cooling water. When the temperature in the tower drops to 35°C, it is further cooled to 30°C naturally, and then inoculated with 10% (v / v) wood Acetobacter and 3% acetic acid bacteria seed liquid, after 12 hours of static culture, poured once every 4 hours, each time for 1 minute...

Embodiment 2

[0029] Example 2: The total sugar content of 171.28g / L saccharification liquid and alcoholic fermentation mash with ethanol content of 8.24% (v / v) were accurately measured at 3:5 (v:v), and 8L of the above mixed solution was added and sterilized by boiling water for 30 minutes At the bottom of the pouring fermentation tower, 7.5kg of corncobs and 5kg of rice husks sterilized at 121°C were transferred to the packing layer above the bottom of the pouring fermentation tower while hot (wherein the corncobs were stacked naturally, and the rice hulls were placed on the bottom of the corncobs and The top part is tiled in two layers), and after the top cover is covered, the jacket is forced to cool with cooling water. When the temperature in the tower drops to 35°C, it is further cooled to 30°C naturally, and then inoculated with 8.2% (v / v) Acetobacter xylinum, 1.9% (v / v) acetic acid bacteria, 2.1% (v / v) yeast seed liquid, with 12h as a control cycle, no oxygen limitation in the first ...

Embodiment 3

[0031] Example 3: The total sugar content of 165g / L saccharification liquid and alcoholic fermentation mash with ethanol content of 7.2% (v / v) were 3:5 (v:v), and 8L of the above mixed solution was accurately measured and added to the sterilized by boiling water for 30 minutes. In the pouring fermentation tower, 7.5 kg of corncobs and 5 kg of rice husks sterilized at 121°C were transferred to the false bottom of the pouring fermentation tower while hot (the corncobs were stacked naturally, and the rice hulls were placed on the bottom and top of the corncobs). After the top cover is covered, the jacket is forced to cool with cooling water. When the temperature in the tower drops to 35°C, it is further naturally cooled to 30°C, and then inoculated with 11% v / v Acetobacter xylinum, taking 12 hours as a control cycle, the first cycle does not limit oxygen, and from the second cycle, the frequency of pouring is 2 times / 12h (of which oxygen is limited for 4h), and each time is poured...

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Abstract

The invention discloses a new method for producing bacterial cellulose. The method comprises the following steps of: accommodating culture medium fermentation liquor in which three strains, namely acetobacter xylinum, acetic acid bacteria and angel yeast are inoculated at the bottom of a pouring fermentation tower which is used for producing vinegar and serves as production equipment by a pouringfermentation method, wherein a culture medium is a mixed solution of stocked rice saccharification liquor and alcoholic fermentation mash; filling corncob and husks in a packing layer on the middle part of the tower, and horizontally laying the husks at the bottom and on the upper part of the corncob, wherein the corncob and the husks are used as thallus attached vectors; lifting the three kinds of strain culture medium fermentation liquor at the bottom of the tower to the top of the tower by using a pump, spraying to the packing layer, returning to the bottom of the tower through the packinglayer, and performing cyclic pouring in the mode at the fermentation temperature of between 28 and 32 DEG C at the frequency of 1 time per 4 hours, wherein the pouring time each time is 1 minute, 12 hours are used as one control cycle, and the total fermentation time is 144 hours; and stopping the pouring fermentation, tearing a bacterial cellulose membrane on the corncob off, washing, and dryingto obtain the bacterial cellulose.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a new method for producing bacterial cellulose. Background technique [0002] Bacterial cellulose (BC), also known as microbial cellulose, is a long and thin bundle of fibers produced by certain microorganisms in the sugar-containing matrix during the growth process and secreted into the matrix. [0003] Bacterial cellulose has good water-holding and gel properties, and can be used in the food industry as a thickener, solid food forming agent, dispersant and binder; it also has good biocompatibility, air permeability and Good physical and chemical properties can be used as a new type of medical biomaterial with unique advantages; the purity, crystallinity, polymerization degree and molecular orientation of bacterial cellulose film are very high, and it has excellent mechanical properties, which makes it suitable for papermaking, Acoustic equipment and building materials c...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/04C12R1/02C12R1/865
Inventor 卢红梅彭湘屏戴锐黄永涛李大鹏王俊张丽平
Owner 贵州国芯生物科技有限公司
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