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Bacterial cellulose culture method based on potato waste residue

A technology of bacterial cellulose and a culture method, which is applied in the field of bacterial cellulose culture, can solve the problems of incompetent price, high cost, low yield and the like, and achieve the effects of shortening the culture time, low cost and high yield

Active Publication Date: 2012-02-01
BEIJING GUANLAN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recycling, potato waste often faces the following problems: 1) Potato waste is highly viscous and difficult to separate solid and liquid; 2) Sterilization of potato waste before recycling is time-consuming and expensive
Although the yield of Acetobacter xylinum is relatively high, it still cannot solve the problem of high production cost of bacterial cellulose
As we all know, in the field of microbial engineering, from small-scale experimental screening to industrial production above the pilot scale, the conditions of each link must be optimized, including the optimization of the culture medium. Experimental conditions are mechanically expanded to large-scale production, and will face controllability issues such as pH value, temperature, and gas required for cultivation, making large-scale production impossible
At present, the main technical obstacles to the industrialization of bacterial cellulose lie in the low level of fermentation, low yield, high cost, and the price is not equal to that of ordinary plant cellulose; further research and utilization of bacterial cellulose film-forming and molding technology have not yet been developed. solve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Preparation of Potato Waste Residue

[0015] The potato waste residue in the present invention is the residue after starch is extracted from fresh potatoes. The specific preparation method is as follows: take potatoes, clean the soil on the surface of the potatoes, and remove foreign objects such as stones and iron filings. Crush the washed potatoes into a paste with a grinder until there is no graininess when twisted by hand. A proper amount of fresh water is injected to accelerate the flow of potato meal, so that starch and potato meal can be separated better. The potato meal is sieved through a flat sieve with a vibration frequency of 130 times / min, and the sieve holes are 30 mesh, 50 mesh, 60 mesh, and 80 mesh from front to back. The humidity is 75%. Other methods can also be used to separate the starch in the potatoes, and then obtain the potato waste residue.

[0016] Remove potato waste residue and heat at 100°C (80°C is also acceptable) for 1 hou...

Embodiment 2

[0018] Example 2 Preparation of culture medium

[0019] Formula 1: Take potato waste residue and add water to heat at 80° C. for 1 hour so that the mass ratio of potato waste residue to distilled water is 2:50 to obtain potato culture solution, the pH of which is 4.0.

[0020] Comparative formula 1: take potato waste residue and add water to heat at 80° C. for 1 hour so that the mass ratio of potato waste residue to distilled water is 2:50 to obtain potato culture solution, the pH of which is 3.5.

[0021] Formula 2: take potato waste residue and add water to heat at 100° C. for 1 hour so that the mass ratio of potato waste residue to distilled water is 11:50 to obtain potato culture solution, the pH of which is 7.5.

[0022] Comparative formula 2: take potato waste residue and add water to heat at 100° C. for 1 hour so that the mass ratio of potato waste residue to distilled water is 11:50 to obtain potato culture solution, the pH of which is 8.0.

[0023] Recipe 3: Specif...

Embodiment 3

[0024] Example 3 One-step preparation of bacterial cellulose

[0025] The Acetobacter xylinum bacterial classification was inoculated in the substratum, and was cultivated in a shaking table under the condition of 180rpm for 3 days, and the gained Acetobacter xylinum bacterial classification was inoculated in the substratum by 7% inoculum, and was cultivated under static conditions for 3 days, and the obtained fermented liquid Transfer to a shallow dish for static cultivation for 5 days to obtain bacterial cellulose. The cultivation temperature is 31° C., and the ventilation volume during cultivation is 0.3vvm. Put the obtained bacterial cellulose into 90°C deionized water to sterilize for 2 hours, take it out and wash it twice with deionized water; boil it with boiling 0.5 mol / L NaOH solution for 15 min; put in 1% NaOH Let it stand in the solution for 2 days; place it in deionized water and soak it until it becomes neutral, and then weigh it. See the column corresponding to...

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Abstract

The invention relates to a bacterial cellulose culture method which concretely comprises the steps of: inoculating acetobacter xylinum strain in a culture medium for recovery; after recovery, inoculating 2-20% of acetobacter xylinum strain in the culture medium; culturing the inoculated acetobacter xylinum strain by a shaking bed for 2-3 days under the condition of 100-180 rpm; and transferring the obtained fermentation liquor into a shallow tray and culturing for 2-5 days in a static state to obtain bacterial cellulose, wherein the culture temperature is 26-31 DEG C, the culture ventilation amount is 0.3-0.6vvm, and the culture medium is potato culture solution. The culture method has the characteristics that: potato waste residue obtained after starch production is firstly taken as the raw material of the culture medium, is treated by a simplest method and then is used as the culture medium for culturing the bacterial cellulose; the culture medium is low in cost and high in yield; meanwhile, the problem of recycling the potato waste residue can be solved, and the culture time is short; and in addition, the invention provides an important condition for stably culturing the bacterial cellulose in a large scale.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for cultivating bacterial cellulose. Background technique [0002] Potatoes are widely distributed, highly adaptable, and easy-to-cultivate crops with high yields. In addition to being used as food, potatoes are mainly used for starch processing. Every ton of starch produced from potatoes will produce 7.5 tons of potato waste. Potato waste residue mainly contains protein, amino acid and a small amount of sugar, which is a recyclable resource. At present, potato waste residue can be recycled for the production of alcohol, feed, degradable plastics, extraction of pectin and preparation of dietary fiber. However, in recycling, potato waste often faces the following problems: 1) Potato waste is highly viscous and difficult to separate solid and liquid; 2) Sterilization of potato waste before recycling is time-consuming and expensive. [0003] Bacterial cellulose is another k...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/02
Inventor 吴力克张娟
Owner BEIJING GUANLAN TECH
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