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Preparation method of aloe polysaccharide/bacterial cellulose composite membrane for medical dressing

A technology of bacterial cellulose film and bacterial cellulose, applied in the field of preparation of aloe polysaccharide/bacterial cellulose composite film, which can solve the problems of poor strength of aloe polysaccharide film and difficulty in covering wounds

Inactive Publication Date: 2017-05-24
南京荣之盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The film strength of aloe polysaccharide is poor, and it is not easy to cover the wound surface. If aloe polysaccharide is directly used as a dressing for wound treatment, it will have certain difficulties

Method used

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  • Preparation method of aloe polysaccharide/bacterial cellulose composite membrane for medical dressing
  • Preparation method of aloe polysaccharide/bacterial cellulose composite membrane for medical dressing
  • Preparation method of aloe polysaccharide/bacterial cellulose composite membrane for medical dressing

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preparation example Construction

[0028] The present invention is used for the preparation method of the aloe polysaccharide / bacterial cellulose composite film of medical dressing, and concrete steps are as follows:

[0029] Step 1, the strain Acetobacter xylinum NUST4.2 is activated;

[0030] Activation of strains: Weigh the required ingredients according to the solid medium formula, add water to make up the volume, adjust to the required pH, and sterilize at 121°C for 20 minutes. After the sterilization, pour the liquid into the sterilized petri dish while it is hot and cool it down to room temperature to obtain a solid medium. The strain Acetobacter xylinum NUST4.2 was placed in a solid medium, and placed in a constant temperature incubator at 30° C. for static culture for 3-5 days to obtain activated bacteria. Among them, solid medium (w / v): glucose 2.0%, sucrose 1.0%, peptone 1.0%, yeast extract powder 0.1%, agar 1.8%, sodium dihydrogen phosphate 0.25%, citric acid 0.11%, magnesium sulfate 0.04%; pH = 6...

Embodiment 1

[0051] Step 1, the activation of strains: according to the solid medium (w / v): glucose 2.0%, sucrose 1.0%, peptone 1.0%, yeast extract powder 0.1%, agar 1.8%, sodium dihydrogen phosphate 0.25%, citric acid 0.11 %, Magnesium Sulfate 0.04%; pH=6.0, weigh the required substances and add water to make up 200ml solution, sterilize, subpackage and cool down, inoculate in a clean bench, place it at 30°C for 3-5 days, and finally get activated bacteria.

[0052] Step 2, preparation of seed liquid: according to the formula of seed medium: glucose 2.0%, ammonium sulfate 0.6%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.04%; peptone 0.3%, yeast extract powder 0.225%, carboxymethyl cellulose Sodium 0.04% Weigh the required components, add water to make up to 50ml, place in a 25% Erlenmeyer flask, sterilize at 121°C for 20min, and cool to 30°C after sterilization to obtain a seed culture medium solution. Then, inoculate the activated strains into the obtained seed culture sol...

Embodiment 2

[0056] Steps 1 and 2 are as above and do not need to be repeated.

[0057] Step 3, the preparation of aloe polysaccharide / bacterial cellulose composite film: according to the fermentation medium formula: glucose 2.25%, sucrose 2.75%, ammonium sulfate 0.1%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.07%, calcium lactate 0.02%, 1.0% peptone, 0.75% yeast extract powder, 0.15% glacial acetic acid, 0.06% citric acid, 0.04% sodium carboxymethyl cellulose, 0.2% or 0.3% aloe polysaccharide, weigh the required ingredients, add water to make up 50ml, and adjust to the desired Need pH, put it in a Erlenmeyer flask with 50% liquid content, sterilize at 121°C for 20 minutes, cool to 30°C after sterilization to obtain fermentation medium solution. The seeds are inserted into the fermentation medium solution according to the inoculation amount of 10% (volume ratio), and then placed in a constant temperature incubator at 30° C. for 7 days.

[0058] Step 4, purification of aloe ...

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Abstract

The invention discloses a preparation method of an aloe polysaccharide / bacterial cellulose composite membrane for a medical dressing. The preparation method comprises the following steps: activating a strain Acetobacter xylinum NUST4.2; preparing a seed solution of the strain Acetobacter xylinum NUST4.2; adding aloe polysaccharide with different concentrations into a fermentation culture medium of the strain Acetobacter xylinum NUST4.2 to carry out dynamic-static combined fermentation production, so as to obtain an aloe polysaccharide / bacterial cellulose composite membrane; purifying the aloe polysaccharide / bacterial cellulose composite membrane; respectively carrying out infrared characterization and scanning electron microscope observation on the structure and morphology of the aloe polysaccharide / bacterial cellulose composite membrane; and carrying out physical performance test on the aloe polysaccharide / bacterial cellulose composite membrane as a medical dressing, so as to show that the requirements of the medical dressing are met. By virtue of the preparation method, more choices are provided for the medical dressing for healing skin wounds, and meanwhile, a positive role for the development of the medical dressing is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, in particular to a method for preparing an aloe polysaccharide / bacterial cellulose composite film used for medical dressings. Background technique [0002] As the largest organ of the human body, the skin is the first line of defense against foreign pathogens and plays an important protective role in the body. When the skin is damaged, it will undoubtedly bring various obstacles to the physiological functions of the human body, and even cause a series of complex pathophysiological changes. However, with the acceleration of industrialization, the number of wounds caused by various accidents is increasing day by day. As an important branch of wounds, skin wounds have also received extensive attention for their effective healing. The healing process of wounded skin is a complex physiological process, and the application of dressing therapy is an effective means to quickly repair wounded skin. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/42A61L15/46
CPCA61L15/28A61L15/425A61L15/46A61L2300/232A61L2300/412C08L1/02
Inventor 梁光芸陈春涛刘长生张衡自强顾焱孙汴京黄洋
Owner 南京荣之盛生物科技有限公司
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