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Method for acquiring asparagus homozygous diploid by culturing anther

A diploid and asparagus technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of serious somatic cell interference, low frequency of haploid pollen, low callus induction rate, etc., to achieve Promote the effect of steady development and stable traits

Inactive Publication Date: 2018-04-06
SHANDONG WEIFANG ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The efficiency of asparagus anther culture is not high, and the callus induction rate is low;
[0006] (2) Severe somatic cell interference and low frequency of pollen haploid

Method used

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  • Method for acquiring asparagus homozygous diploid by culturing anther
  • Method for acquiring asparagus homozygous diploid by culturing anther
  • Method for acquiring asparagus homozygous diploid by culturing anther

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 A method for obtaining asparagus homozygous diploid by anther culture

[0033] The main programs are as follows:

[0034] 1. Asparagus anther culture steps

[0035] 1.1 Acquisition of asparagus anthers

[0036] From 8:00 to 10:00 in the morning from April to May every year, 20 male inflorescences of different single plants were obtained from the field and marked respectively. After the male inflorescences of asparagus were retrieved, buds with a length of about 1-2mm were selected. Microscopic examination showed that, At this time, the pollen is in the mononuclear border stage.

[0037] The asparagus plant variety is 'Champion'.

[0038] 1.2 Pretreatment of asparagus anthers

[0039] Put the asparagus buds in a 4°C refrigerator for low-temperature pretreatment for 2.5-3.5 days, soak the buds in 75% alcohol solution on the ultra-clean workbench for 10s, rinse with sterile water for 3 times, and soak them in 0.2% mercuric solution for 15 seconds. min, rinse...

Embodiment 2

[0054] Example 2 Test results of other asparagus varieties

[0055] The method of obtaining asparagus homozygous diploid by using anther culture described in Example 1 was adopted, and the varieties of asparagus were changed, and Lu Asparagus No. 1, Grande, and Jinglu No. 1 were respectively used.

[0056] surface Anther callus induction rate (%)

[0057]

[0058] The above-mentioned callus is a hard and compact callus.

[0059] surface Germination rate and multiplication coefficient of different varieties

[0060]

[0061] surface Effective rooting rate of different varieties (%)

[0062]

[0063] surface Haploid, diploid, tetraploid and aneuploid status of differentiated green shoots in different varieties (%)

[0064]

[0065] surface Success rate of haploid chromosome induction in different varieties (%)

[0066]

[0067] Table 6 Results of haploid chromosome doubling in different varieties

[0068]

Embodiment 3

[0069] Example 3 Effects of different anther collection times on callus induction rate

[0070] Using the method described in Example 1, only the anthers in the step "1.1 Acquisition of Asparagus Anthers" were changed

[0071] The time of collection and the variety of asparagus were tested.

[0072] In Weifang area, the flowering period of asparagus lasted until September to October. Therefore, the anthers of different months were collected from April to September in 2010 to 2015, and the callus induction rate of anthers at different collection times was studied. The results showed that the anthers from April to May The induction rate was the highest, followed by June, and lower inoculated after July. The four varieties used in the experiment showed this regularity (Table 7).

[0073] Table 7 Induction rate of anther callus in different periods (%)

[0074]

[0075] The above-mentioned callus refers to the hard and compact callus

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Abstract

The invention provides a method for acquiring asparagus homozygous diploid by culturing anther. The method comprises the following steps: acquiring asparagus anther, pre-treating the asparagus anther,performing callus induction for the asparagus anther, performing sub-generation differentiation for the asparagus anther, rooting a test-tube seedling, and multiplying haploid chromosome. By adoptingthe method for acquiring the asparagus homozygous diploid by culturing anther, the induction rate of callus with hard and compact texture of the asparagus anther is 7.2 to 17 percent; and the callusinduction rate of the variety ''champion'' with hard and compact texture is 15.8 to 17 percent or higher. According to the method for acquiring asparagus homozygous diploid by culturing anther, the haploid differentiation rate is 2.2 to 7.2 percent, the success rate for obtaining the homozygous diploid and multiploid by inducing the haploid is 45.86 to 49.73 percent, and the ratio of the homozygous diploid is 1.6 to 2.9 percent.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for cultivating homozygous diploid super-male plants and female plants through an asparagus flower culture approach. Background technique [0002] Asparagus scientific name Shi Diaobai ( Asparagus officinalis L.), tender stems have fine texture, soft and delicious fibers, delicious flavor and unique aromatic flavor. It is rich in polysaccharides, flavonoids, saponins and other active ingredients. Asparagus has the functions of anti-tumor, anti-fatigue, lowering blood pressure, lowering blood lipids, and improving immune function. One of the "Top Ten Famous Dishes", it enjoys the reputation of "King of Vegetables" in the international market. [0003] At present, the method of breeding conventional asparagus varieties is as follows: firstly select male and female plants with excellent traits for hybridization; select strong heterosis combinations, and then condu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/08
CPCA01H4/008A01H1/08
Inventor 李霞李保华包艳存杨林牟萌牛晓雪
Owner SHANDONG WEIFANG ACADEMY OF AGRICULTURAL SCIENCES
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