A kind of breeding method of high temperature resistant sea cucumber strain

A technology of high temperature resistance and sea cucumber, applied in application, climate change adaptation, fish farming and other directions, can solve the problems of sea cucumber impact, long duration, and high water temperature in sea cucumber ponds, achieve stable high temperature resistance properties, and improve economic benefits. , the effect of improving the selection efficiency

Active Publication Date: 2019-03-01
山东省海洋资源与环境研究院
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Problems solved by technology

However, with the change of global climate, while the global average temperature is rising, extreme climates such as high temperature in summer are also appearing more frequently in many regions of the world. The duration is long, and the surface water temperature of ponds in some areas can reach above 32°C, which has a serious impact on the cultivation of sea cucumbers in ponds. A large number of sea cucumbers rot and die, and only a few individuals survive
According to reports, in the summer of 2013, sea cucumber farming along the coast of Shandong suffered from continuous high-temperature disasters. Dongying, Muping, Laizhou, Rushan and other places have caused large-scale death disasters, direct economic losses of billions of yuan, and brought heavy losses to sea cucumber farmers. The blow has seriously affected the healthy development of sea cucumber aquaculture in summer

Method used

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Embodiment Construction

[0019] The present invention is specifically introduced below in conjunction with specific embodiment:

[0020] 1) Apostichopus japonicus parents are surviving individuals collected from ponds in Yantai, Qingdao, and Dongying after high temperature elimination in summer. The pond water temperature is above 32°C and the high temperature lasts for 5 days, and the survival rate of elimination is <0.05%. 100 heads of each of the three groups were used as parents, and were bred to sexual maturity. The method of artificial cold water drainage was used to breed ginseng. Based on the principle of 1 female ginseng and 1-3 male ginseng, more than 60 full-sib families and half-sib families were established. 72 families were established, and the breeding tanks of each family were isolated and cultivated;

[0021] 2) Each family of sea cucumbers develops to the stage of ear-shaped larvae. Take 1 L of sea cucumber larvae (density 0.5 inds / mL) from each family in a glass beaker, place them i...

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Abstract

The invention discloses a method for selecting and breeding high-temperature-resistant sea cucumber strains, belonging to the field of aquatic genetics and breeding. The present invention selects surviving sea cucumbers eliminated by natural high temperature in summer in different regions as parents, breeds and constructs families after sexual maturity, conducts heat shock at 28°C at the stage of auricular larvae in each family, and retains 20% of the families according to the survival rate and deformity rate, and then Keeping the mixed culture of the family until the average body weight of the individual is >50g, carry out high temperature stress at 32°C, remove the dead and damaged individuals, keep ≤25% of the individuals and continue to breed until sexual maturity, continue heat shock selection and breeding for three generations in the same principle, and obtain A new strain of sea cucumber with high temperature resistance. The original parent of japonicus japonicus of the present invention is more in line with the tolerance characteristics of the actual breeding environment; the screening of family high temperature domestication in the larvae stage and the screening of high temperature stress in the breeding stage, the combination of family selection and group selection improves the selection efficiency; continuous three-generation selection ensures stable high temperature resistance traits , has good economic benefits and broad breeding prospects.

Description

technical field [0001] The invention relates to a method for breeding high-temperature-resistant sea cucumber strains, belonging to the field of aquatic genetics and breeding. Background technique [0002] Apostichopus aquaculture is an important marine aquaculture industry in northern my country. In recent years, with people's understanding of the nutritional value of sea cucumbers, the market demand for sea cucumber products has continued to heat up. The scale of sea cucumber farming has expanded rapidly with the increase in market demand. At present, the scale of sea cucumber farming has reached several million mu, with an annual output value of nearly 20 billion It is the species with the highest output value of a single species in mariculture in my country. However, with the change of global climate, while the global average temperature is rising, extreme climates such as high temperature in summer are also appearing more frequently in many regions of the world. The du...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/30
CPCY02A40/81
Inventor 孙国华杨建敏任利华王卫军杨爽
Owner 山东省海洋资源与环境研究院
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