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Breeding method of high-temperature-resistant stichopus japonicus strain

A technology of high temperature resistance and japonicus, applied in fish farming, application, climate change adaptation, etc., can solve the problems of japonicus impact, long duration, high water temperature in japonicus ponds, etc., to improve selection efficiency and stabilize high temperature resistant properties , the effect of improving economic efficiency

Active Publication Date: 2017-01-04
山东省海洋资源与环境研究院
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AI Technical Summary

Problems solved by technology

However, with the change of global climate, while the global average temperature is rising, extreme climates such as high temperature in summer are also appearing more frequently in many regions of the world. The duration is long, and the surface water temperature of ponds in some areas can reach above 32°C, which has a serious impact on the cultivation of sea cucumbers in ponds. A large number of sea cucumbers rot and die, and only a few individuals survive
According to reports, in the summer of 2013, sea cucumber farming along the coast of Shandong suffered from continuous high-temperature disasters. Dongying, Muping, Laizhou, Rushan and other places have caused large-scale death disasters, direct economic losses of billions of yuan, and brought heavy losses to sea cucumber farmers. The blow has seriously affected the healthy development of sea cucumber aquaculture in summer

Method used

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Embodiment Construction

[0019] The present invention is specifically introduced below in conjunction with specific embodiment:

[0020] 1) Apostichopus japonicus parents are surviving individuals collected from ponds in Yantai, Qingdao, and Dongying after high temperature elimination in summer. The pond water temperature is above 32°C and the high temperature lasts for 5 days, and the survival rate of elimination is <0.05%. 100 heads of each of the three groups were used as parents, and were bred to sexual maturity. The method of artificial cold water drainage was used to breed ginseng. Based on the principle of 1 female ginseng and 1-3 male ginseng, more than 60 full-sib families and half-sib families were established. 72 families were established, and the breeding tanks of each family were isolated and cultivated;

[0021] 2) Each family of sea cucumbers develops to the stage of ear-shaped larvae. Take 1 L of sea cucumber larvae (density 0.5 inds / mL) from each family in a glass beaker, place them i...

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Abstract

The invention discloses a breeding method of a high-temperature-resistant stichopus japonicus strain and belongs to the field of genetic breeding of aquatic products. Stichopus japonicus surviving after natural high temperature elimination in summer in different regions is selected as a parent, families are constructed through breeding after sexual maturity, heat shock at the temperature of 28 DEG C is performed at the auricularia stage of each family, 20% of families are reserved according to the survival rate and the malformation rate, the reserved families are mixed and bred until the average weight of individuals is larger than 50 g, high-temperature stress is performed at 32 DEG C to remove dead and damaged individuals, less than 25% of individuals are reserved and continuously bred until sexual maturity, heat shock selection and breeding of three generations are performed continuously in the same principle, and the new stichopus japonicus strain with the high-temperature-resistant character is obtained. The original parent of stichopus japonicus better conforms to the tolerance characteristic of the actual breeding environment; high-temperature domestication screening of the families in the larva period and high-temperature stress screening in the breeding period are performed, family breeding and group breeding are combined, and the selection efficiency is improved; breeding of three generations is performed continuously, the high-temperature-resistant character is stabilized, and the breeding method has good economic benefit and broad breeding prospect.

Description

technical field [0001] The invention relates to a method for breeding high-temperature-resistant sea cucumber strains, belonging to the field of aquatic genetics and breeding. Background technique [0002] Apostichopus aquaculture is an important marine aquaculture industry in northern my country. In recent years, with people's understanding of the nutritional value of sea cucumbers, the market demand for sea cucumber products has continued to heat up. The scale of sea cucumber farming has expanded rapidly with the increase in market demand. At present, the scale of sea cucumber farming has reached several million mu, with an annual output value of nearly 20 billion It is the species with the highest output value of a single species in mariculture in my country. However, with the change of global climate, while the global average temperature is rising, extreme climates such as high temperature in summer are also appearing more frequently in many regions of the world. The du...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 孙国华杨建敏任利华王卫军杨爽
Owner 山东省海洋资源与环境研究院
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