Breeding method of high-temperature-resistant stichopus japonicus strain
A technology of high temperature resistance and japonicus, applied in fish farming, application, climate change adaptation, etc., can solve the problems of japonicus impact, long duration, high water temperature in japonicus ponds, etc., to improve selection efficiency and stabilize high temperature resistant properties , the effect of improving economic efficiency
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[0019] The present invention is specifically introduced below in conjunction with specific embodiment:
[0020] 1) Apostichopus japonicus parents are surviving individuals collected from ponds in Yantai, Qingdao, and Dongying after high temperature elimination in summer. The pond water temperature is above 32°C and the high temperature lasts for 5 days, and the survival rate of elimination is <0.05%. 100 heads of each of the three groups were used as parents, and were bred to sexual maturity. The method of artificial cold water drainage was used to breed ginseng. Based on the principle of 1 female ginseng and 1-3 male ginseng, more than 60 full-sib families and half-sib families were established. 72 families were established, and the breeding tanks of each family were isolated and cultivated;
[0021] 2) Each family of sea cucumbers develops to the stage of ear-shaped larvae. Take 1 L of sea cucumber larvae (density 0.5 inds / mL) from each family in a glass beaker, place them i...
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