Strain YAB-3 for degrading PFOA (perfluorooctanoic acid) and application thereof

A YAB-3, strain technology, applied in bacteria, biochemical equipment and methods, microorganisms, etc., can solve problems such as weak degradation ability, inability to meet environmental pollution control, scarcity of bacterial resources, etc. Effect

Inactive Publication Date: 2015-10-14
JISHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

As far as the current research is concerned, although some microorganisms that degrade PFOA and other perfluorinated compounds have been isolated and screened from nature, the degradation ability is very weak, and the strain resources are very scarce, which cannot meet the needs of environmental pollution control.

Method used

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  • Strain YAB-3 for degrading PFOA (perfluorooctanoic acid) and application thereof

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Embodiment Construction

[0023] The PFOA-degrading strain YAB-3 and its application are further specifically described in conjunction with specific examples.

[0024] The inventor collected a sample containing PFOA on April 13, 2013 in the soil around the Wuhan fluoride factory and the activated sludge of the sewage treatment center, and isolated a PFOA-degrading strain YAB-3 from the sample ( Sphingomonas yanoikuyae YAB-3), deposit number: CCTCC NO: M2015281, deposit date: May 7, 2015, depository unit: China Center for Type Culture Collection, deposit address: Wuhan University, Wuhan, China.

[0025] Using strains ( Sphingomonas yanoikuyae YAB-3) The method of degrading PFOA-containing materials according to the following method has a better effect: first inoculate a single colony of YAB-3 into LB medium for 15 hours, then make this medium into a certain OD bacterial suspension, and finally use this Bacterial suspension to treat materials containing PFOA.

[0026] The results of strain identifica...

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Abstract

The invention discloses a strain YAB-3 for degrading PFOA (perfluorooctanoic acid) and an application thereof. The strain for degrading PFOA is sphingomonas yanoikuyae, with the collection number of CCTCC (China Center for Type Culture Collection) NO:M2015281, the collection date of May 7, 2015 and the collection unit of CCTCC. A method for degrading PFOA-containing materials by utilizing the strain YAB-3 is characterized by comprising the following steps: firstly inoculating the single colony YAB-3 into an LB culture medium for 12-18 hours, then preparing bacterial suspension with certain OD600 with the culture medium, and finally treating the PFOA-containing materials with the bacterial suspension. The invention provides the strain YAB-3 for degrading PFOA and the method for treating the PFOA-containing materials by utilizing the strain. The strain has strong capability of degrading PFOA. The method for treating the PFOA-containing materials is simple and effective and can achieve the effect of obviously reducing the concentration of PFOA in the materials.

Description

technical field [0001] The invention belongs to the technical field of biology and environmental protection, and specifically refers to a PFOA degrading bacterial strain YAB-3 and a method for degrading PFOA using YAB-3. Background technique [0002] Perfluorinated compounds (Perfluorinated compounds, PFCs) refer to straight-chain or branched-chain hydrocarbons in which all hydrogen atoms are replaced by fluorine. Due to the high bond energy of the C-F bond, it has strong chemical, biological and thermal stability. It is difficult to be naturally degraded in the environment. [0003] Perfluorooctane acid (PFOA) has typical structural characteristics and chemical properties of PFCs. It is not only widely used in traditional industries such as textiles, leather, chemicals, machinery and medicine, but also in high-tech fields such as aerospace and semiconductors in recent years. , microelectronics and military industry, etc. At present, there is no environmental medium with z...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/28
CPCA62D3/02A62D2101/28C12N1/205C12R2001/01
Inventor 彭清忠易浪波彭清静
Owner JISHOU UNIVERSITY
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